Survey entry #5

CRK3:CYC6

Author of entry: Jeremy Mottram   |   Organism: Trypanosoma brucei   |   Date submitted: 2007-06-26

Gene links: Tb927.10.4990.

Description:
CRK3:CYC6 is a cyclin-dependent protein kinase that is thought to be the functional homologue of mammalian CDK1:CYCB, known to be essential for entry into mitosis. Both CRK3 and CYC6 have been shown to be essential for bloodstream form T. brucei using RNAi (Hammarton et al., 2003; Tu et al., 2004). The Leishmania CRK3 and CYC6 genes have been expressed in E. coli and CRK3:CYC6 purified as an active kinase complex. An HTS assay has been developed using IMAP technology and a screen of 25,000 compounds undertaken. Efforts to make active recombinant T. brucei CRK3:CYC6 are in progress Hammarton,T.C., Clark,J., Douglas,F., Boshart,M., and Mottram,J.C. (2003). Stage-specific differences in cell cycle control in Trypanosoma brucei revealed by RNA interference of a mitotic cyclin. J. Biol. Chem. 278, 22877-22886. Tu,X. and Wang,C.C. (2004). The involvement of two cdc2-related kinases (CRKs) in Trypanosoma brucei cell-cycle regulation and the distinctive stage-specific phenotypes caused by CRK3 depletion. J. Biol. Chem. 279, 20519-20528. Target ID: geneDB number = Tb10.70.2210 EC number (if enzyme) = target name = CRK3 Assay: No convenient assay at present Availability: No useful expression system is available Activity: Small (< 1 full time person)

Target ID: geneDB number = Tb10.70.2210 EC number (if enzyme) = target name = CRK3

Validation: Genetic OR chemical evidence

Assay status: No convenient assay at present

Availability: No useful expression system is available

Activity: Small (< 1 full time person)

References:

Stage-specific differences in cell cycle control in Trypanosoma brucei revealed by RNA interference of a mitotic cyclin. (2003). J Biol Chem 278: 22877-86. PubMed, Publisher.

The involvement of two cdc2-related kinases (CRKs) in Trypanosoma brucei cell cycle regulation and the distinctive stage-specific phenotypes caused by CRK3 depletion. (2004). J Biol Chem 279: 20519-28. PubMed, Publisher.