Detailed view for Tb927.1.870

Basic information

TDR Targets ID: 10156
Trypanosoma brucei, deoxyhypusine synthase

Source Database / ID: TriTrypDB / Tb927.1.870  GeneDB / Tb927.1.870

pI: 6.3371 | Length (AA): 342 | MW (Da): 37378 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: ND

Pfam domains

PF01916   Deoxyhypusine synthase

Gene Ontology

Mouse over links to read term descriptions.
GO:0050983   spermidine catabolic process to deoxyhypusine, using deoxyhypusine synthase  
GO:0008612   peptidyl-lysine modification to hypusine  

Network

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models: 2

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 341 1rlz (A) 22 367 40.00 0 1 1.46335 -0.47
26 332 4p63 (A) 28 336 37.00 0 1 1.37586 -0.76

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127351)
Species Accession Gene Product
Arabidopsis thaliana AT5G05920   deoxyhypusine synthase
Babesia bovis BBOV_III010890   deoxyhypusine synthase, putative
Brugia malayi Bm1_16300   deoxyhypusine synthase
Candida albicans CaO19.1626   deoxyhypusine synthase
Candida albicans CaO19.9194   deoxyhypusine synthase
Caenorhabditis elegans CELE_Y17G7B.4   Protein DHPS-1
Cryptosporidium hominis Chro.20420   deoxyhypusine synthase
Cryptosporidium parvum cgd2_3930   deoxyhypusine synthase
Dictyostelium discoideum DDB_G0285725   deoxyhypusine synthase
Drosophila melanogaster Dmel_CG8005   CG8005 gene product from transcript CG8005-RA
Echinococcus granulosus EgrG_000780100   deoxyhypusine synthase
Entamoeba histolytica EHI_098350   deoxyhypusine synthase, putative
Entamoeba histolytica EHI_006030   deoxyhypusine synthase, putative
Echinococcus multilocularis EmuJ_000780100   deoxyhypusine synthase
Giardia lamblia GL50803_15535   Deoxyhypusine synthase, putative
Homo sapiens ENSG00000095059   deoxyhypusine synthase
Leishmania braziliensis LbrM.20.4450   deoxyhypusine synthase, putative
Leishmania donovani LdBPK_200270.1   deoxyhypusine synthase-like
Leishmania infantum LinJ.20.0270   deoxyhypusine synthase-like
Leishmania major LmjF.20.0250   deoxyhypusine synthase, putative
Leishmania mexicana LmxM.20.0250   deoxyhypusine synthase, putative
Loa Loa (eye worm) LOAG_12575   deoxyhypusine synthase
Mus musculus ENSMUSG00000060038   deoxyhypusine synthase
Neospora caninum NCLIV_050000   Probable deoxyhypusine synthase (EC 2.5.1.46), related
Oryza sativa 4334056   Os03g0740600
Plasmodium berghei PBANKA_1030000   deoxyhypusine synthase
Plasmodium falciparum PF3D7_1412600   deoxyhypusine synthase
Plasmodium knowlesi PKNH_1345700   deoxyhypusine synthase, putative
Plasmodium vivax PVX_085825   deoxyhypusine synthase, putative
Plasmodium yoelii PY01546   Deoxyhypusine synthase
Saccharomyces cerevisiae YHR068W   Dys1p
Schistosoma japonicum Sjp_0017580   ko:K00809 deoxyhypusine synthase [EC2.5.1.46], putative
Schistosoma mansoni Smp_065120   deoxyhypusine synthase
Schmidtea mediterranea mk4.001517.00   Putative deoxyhypusine synthase
Schmidtea mediterranea mk4.001513.01   Putative deoxyhypusine synthase
Schmidtea mediterranea mk4.001517.01   Putative deoxyhypusine synthase
Trypanosoma brucei gambiense Tbg972.1.280   deoxyhypusine synthase, putative
Trypanosoma brucei Tb927.1.870 this record   deoxyhypusine synthase
Trypanosoma congolense TcIL3000_1_360   deoxyhypusine synthase, putative
Trypanosoma cruzi TcCLB.511421.60   deoxyhypusine synthase, putative
Toxoplasma gondii TGME49_235620   deoxyhypusine synthase
Theileria parva TP02_0058   deoxyhypusine synthase, putative
Trichomonas vaginalis TVAG_359990   deoxyhypusine synthase, putative

Essentiality

Tb927.1.870 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.870 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.1.870 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.870 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.1.870 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y17G7B.4 Caenorhabditis elegans embryonic lethal wormbase
YHR068W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1030000 Plasmodium berghei Essential plasmo
TGME49_235620 Toxoplasma gondii Probably essential sidik

Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
 
In any case, if you have information about papers containing relevant validation data for this target, please log in using your TDR Targets username and password and send them to us using the corresponding form in this page (only visible to registered users) or contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.8

Curated from literature

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus Deoxyhypusine synthase Compounds References
Plasmodium falciparum Deoxyhypusine synthase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.1.870 (Trypanosoma brucei), deoxyhypusine synthase
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