Detailed view for Tb927.10.1630

Basic information

TDR Targets ID: 10758
Trypanosoma brucei, atp-binding cassette sub-family e member 1
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.3159 | Length (AA): 631 | MW (Da): 71079 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00005   ABC transporter
PF00037   4Fe-4S binding domain
PF04068   Possible Fer4-like domain in RNase L inhibitor, RLI

Gene Ontology

Mouse over links to read term descriptions.
GO:0009055   electron carrier activity  
GO:0005524   ATP binding  
GO:0000166   nucleotide binding  
GO:0051536   iron-sulfur cluster binding  
GO:0017111   nucleoside-triphosphatase activity  
GO:0016887   ATPase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
13 605 3bk7 (A) 3 593 47.00 0 1 1.56008 -0.59
89 606 3ozx (A) 76 596 42.00 0 1 1.40922 -0.97

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127149)

Species Accession Gene Product
Arabidopsis thaliana AT4G19210   RNAse l inhibitor protein 2
Arabidopsis thaliana AT3G13640   ABC transporter E family member 1
Babesia bovis BBOV_III009450   ABC transporter, ATP-binding domain containing protein
Brugia malayi Bm1_15645   ATP-binding cassette sub-family E member 1 (Ribonuclease 4 inhibitor)
Candida albicans CaO19.10552   RNAse L inhibitor-type ATP binding cassette protein similar to S. cerevisiae RLI1 (YDR091C) and to mammalian BCRP transporter
Candida albicans CaO19.3034   RNAse L inhibitor-type ATP binding cassette protein similar to S. cerevisiae RLI1 (YDR091C) and to mammalian BCRP transporter
Caenorhabditis elegans CELE_Y39E4B.1   Protein ABCE-1
Cryptosporidium hominis Chro.10119   RNase L inhibitor-like protein
Cryptosporidium parvum cgd1_980   RNase L inhibitor-like protein
Dictyostelium discoideum DDB_G0290483   4Fe-4S ferredoxin, iron-sulfur binding domain-containing protein
Drosophila melanogaster Dmel_CG5651   pixie
Echinococcus granulosus EgrG_001052900   ATP binding cassette sub family E
Entamoeba histolytica EHI_156200   RNAseL inhibitor-like protein, putative
Echinococcus multilocularis EmuJ_001052900   ATP binding cassette sub family E
Giardia lamblia GL50803_7662   RNase L inhibitor
Homo sapiens ENSG00000164163   ATP-binding cassette, sub-family E (OABP), member 1
Leishmania braziliensis LbrM.21.0770   ribonuclease L inhibitor, putative,ATP-binding cassette sub-family E, putative
Leishmania donovani LdBPK_210770.1   ATP-binding cassette protein subfamily E, member 1, putative
Leishmania infantum LinJ.21.0770   ATP-binding cassette protein subfamily E, member 1, putative
Leishmania major LmjF.21.0710   ATP-binding cassette protein subfamily E, member 1, putative
Leishmania mexicana LmxM.21.0710   ribonuclease L inhibitor, putative,ATP-binding cassette sub-family E, putative
Loa Loa (eye worm) LOAG_04200   ATP-binding cassette subfamily E
Mus musculus ENSMUSG00000058355   ATP-binding cassette, sub-family E (OABP), member 1
Neospora caninum NCLIV_059510   RNase L inhibitor, related
Oryza sativa 4350692   Os11g0546000
Oryza sativa 4329031   Os02g0282900
Plasmodium berghei PBANKA_1144100   ABC transporter E family member 1, putative
Plasmodium falciparum PF3D7_1368200   ABC transporter E family member 1, putative
Plasmodium knowlesi PKNH_1103000   ABC transporter E family member 1, putative
Plasmodium vivax PVX_115370   ABC transporter E family member 1, putative
Plasmodium yoelii PY04219   68 kDa protein
Saccharomyces cerevisiae YDR091C   Rli1p
Schistosoma japonicum Sjp_0011870   ko:K06174 ATP-binding cassette, sub-family E, member 1, putative
Schistosoma mansoni Smp_124460   ATP-binding cassette sub-family E (oabp) member
Trypanosoma brucei gambiense Tbg972.10.1920   ribonuclease L inhibitor, putative,ATP-binding cassette sub-family E, putative
Trypanosoma brucei Tb927.10.1630   atp-binding cassette sub-family e member 1
Trypanosoma congolense TcIL3000_10_1400   ribonuclease L inhibitor, putative
Trypanosoma cruzi TcCLB.508637.150   ribonuclease L inhibitor, putative
Toxoplasma gondii TGME49_216790   ATP-binding cassette sub-family E member 1, putative
Theileria parva TP04_0855   RNAse L inhibitor, putative
Trichomonas vaginalis TVAG_249850   RNAse L inhibitor, putative

Essentiality

Tb927.10.1630 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.1630 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.1630 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.1630 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.1630 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y39E4B.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y39E4B.1 Caenorhabditis elegans larval arrest wormbase
CELE_Y39E4B.1 Caenorhabditis elegans slow growth wormbase
CELE_Y39E4B.1 Caenorhabditis elegans sterile wormbase
YDR091C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1144100 Plasmodium berghei Essential plasmo
TGME49_216790 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 14668021
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 14668021
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.1630 (Trypanosoma brucei), atp-binding cassette sub-family e member 1
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