Detailed view for Tb05.5K5.150

Basic information

TDR Targets ID: 11170
Trypanosoma brucei, small GTP-binding protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.4209 | Length (AA): 194 | MW (Da): 21771 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00025   ADP-ribosylation factor family

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0005525   GTP binding  
GO:0007264   small GTPase mediated signal transduction  
GO:0006886   intracellular protein transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
10 192 1f6b (A) 15 198 54.00 0 1 1.6255 -1.34
21 191 1m2o (B) 24 190 61.00 0 1 1.69024 -1.49

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127331)

Species Accession Gene Product
Arabidopsis thaliana AT3G62560   Ras-related small GTP-binding family protein
Arabidopsis thaliana AT1G56330   GTP-binding protein SAR1B
Arabidopsis thaliana AT4G02080   GTP-binding protein SAR1A
Babesia bovis BBOV_II004300   small GTP-binding protein sar1
Brugia malayi Bm1_36820   GTP-binding protein SAR1
Caenorhabditis elegans CELE_ZK180.4   Protein SAR-1
Cryptosporidium hominis Chro.70265   small GTP-binding protein sar1
Cryptosporidium parvum cgd7_2330   SAR1-like small GTpase
Dictyostelium discoideum DDB_G0272296   ARF/SAR superfamily protein
Drosophila melanogaster Dmel_CG7073   Sar1 ortholog (S. cerevisiae)
Echinococcus granulosus EgrG_000100200   GTP binding protein SAR1b
Entamoeba histolytica EHI_075210   GTP-binding protein
Entamoeba histolytica EHI_031410   GTP-binding protein
Entamoeba histolytica EHI_075040   GTP-binding protein
Echinococcus multilocularis EmuJ_000100200   GTP binding protein SAR1b
Giardia lamblia GL50803_7569   GTP-binding protein Sar1
Homo sapiens ENSG00000152700   secretion associated, Ras related GTPase 1B
Homo sapiens ENSG00000079332   secretion associated, Ras related GTPase 1A
Leishmania braziliensis LbrM.05.0030   ras-like small GTPases, putative
Leishmania donovani LdBPK_050030.1   ras-like small GTPases, putative
Leishmania infantum LinJ.05.0030   ras-like small GTPases, putative
Leishmania major LmjF.05.0030   ras-like small GTPases, putative
Leishmania mexicana LmxM.05.0030   small GTP-binding protein, putative
Loa Loa (eye worm) LOAG_01165   hypothetical protein
Mus musculus ENSMUSG00000020386   SAR1 gene homolog B (S. cerevisiae)
Mus musculus ENSMUSG00000020088   SAR1 gene homolog A (S. cerevisiae)
Neospora caninum NCLIV_052070   hypothetical protein
Oryza sativa 4352510   Os12g0560300
Oryza sativa 4326797   Os01g0338000
Oryza sativa 4327708   Os01g0254000
Oryza sativa 4340541   Os06g0225000
Plasmodium berghei PBANKA_0718800   small GTP-binding protein sar1, putative
Plasmodium falciparum PF3D7_0416800   small GTP-binding protein sar1
Plasmodium knowlesi PKNH_0509200   small GTP-binding protein sar1, putative
Plasmodium yoelii PY04367   small GTP-binding protein
Saccharomyces cerevisiae YPL218W   Arf family GTPase SAR1
Schistosoma japonicum Sjp_0104110   ko:K07954 SAR1B, putative
Schistosoma mansoni Smp_073590   GTP-binding protein-like protein
Schmidtea mediterranea mk4.020490.00   GTP-binding protein-like protein
Schmidtea mediterranea mk4.002137.04   GTP-binding protein-like protein
Trypanosoma brucei gambiense Tbg972.5.6040   ADP-ribosylation factor, putative
Trypanosoma brucei Tb927.5.4500   ras-like small GTPase, putative
Trypanosoma brucei Tb05.5K5.150   small GTP-binding protein, putative
Trypanosoma congolense TcIL3000_0_58560   small GTP-binding protein, putative
Trypanosoma congolense TcIL3000_5_5190   ADP-ribosylation factor, putative
Trypanosoma cruzi TcCLB.509539.50   small GTP-binding protein, putative
Toxoplasma gondii TGME49_215060   small GTP-binding protein sar1, putative
Theileria parva TP04_0542   GTP-binding protein, putative
Trichomonas vaginalis TVAG_222520   ADP-ribosylation factor, arf, putative
Trichomonas vaginalis TVAG_157300   ADP-ribosylation factor, arf, putative

Essentiality

Tb05.5K5.150 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.4500 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.4500 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.4500 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.4500 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_ZK180.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_ZK180.4 Caenorhabditis elegans larval arrest wormbase
CELE_ZK180.4 Caenorhabditis elegans sterile wormbase
YPL218W Saccharomyces cerevisiae inviable yeastgenome
TGME49_215060 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Tb05.5K5.150 (Trypanosoma brucei), small GTP-binding protein, putative
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