Detailed view for Tb927.9.6290

Basic information

TDR Targets ID: 12376
Trypanosoma brucei, arginine kinase
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.4014 | Length (AA): 356 | MW (Da): 40197 | Paralog Number: 2

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00217   ATP:guanido phosphotransferase, C-terminal catalytic domain
PF02807   ATP:guanido phosphotransferase, N-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016772   transferase activity, transferring phosphorus-containing groups  
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0016301   kinase activity  
GO:0003824   catalytic activity  

Metabolic Pathways

Arginine and proline metabolism (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128330)

Species Accession Gene Product
Brugia malayi Bm1_37040   ATP:guanido phosphotransferase, C-terminal catalytic domain containing protein
Brugia malayi Bm1_23610   arginine kinase
Caenorhabditis elegans CELE_W10C8.5   Protein W10C8.5
Caenorhabditis elegans CELE_F32B5.1   Protein F32B5.1
Caenorhabditis elegans CELE_F46H5.3   Protein F46H5.3, isoform B
Caenorhabditis elegans CELE_F44G3.2   Protein F44G3.2
Caenorhabditis elegans CELE_ZC434.8   Protein ZC434.8
Chlamydia trachomatis CT_675   arginine kinase
Drosophila melanogaster Dmel_CG32031   Arginine kinase
Homo sapiens ENSG00000104879   creatine kinase, muscle
Homo sapiens ENSG00000166165   creatine kinase, brain
Loa Loa (eye worm) LOAG_10519   arginine kinase
Loa Loa (eye worm) LOAG_13628   hypothetical protein
Loa Loa (eye worm) LOAG_07634   ATP:guanido phosphotransferase
Mus musculus ENSMUSG00000001270   creatine kinase, brain
Mus musculus ENSMUSG00000030399   creatine kinase, muscle
Onchocerca volvulus OVOC4467  
Onchocerca volvulus OVOC2321  
Schistosoma japonicum Sjp_0200590   ko:K00933 creatine kinase [EC2.7.3.2], putative
Schistosoma japonicum Sjp_0124500   ATP:guanidino kinase SMC74, putative
Schistosoma mansoni Smp_194770   ATP:guanidino kinase (Smc74)
Schmidtea mediterranea mk4.004791.03  
Schmidtea mediterranea mk4.018687.00  
Schmidtea mediterranea mk4.002159.01  
Schmidtea mediterranea mk4.008532.00  
Schmidtea mediterranea mk4.001156.04  
Schmidtea mediterranea mk4.000513.00  
Schmidtea mediterranea mk4.007131.02  
Schmidtea mediterranea mk4.007502.01  
Trypanosoma brucei gambiense Tbg972.9.3300   arginine kinase
Trypanosoma brucei gambiense Tbg972.9.3260   arginine kinase
Trypanosoma brucei gambiense Tbg972.9.3280   arginine kinase
Trypanosoma brucei gambiense Tbg972.9.3250   arginine kinase
Trypanosoma brucei Tb927.9.6210   arginine kinase
Trypanosoma brucei Tb927.9.6250   arginine kinase
Trypanosoma brucei Tb927.9.6290   arginine kinase
Trypanosoma congolense TcIL3000_0_36630   arginine kinase
Trypanosoma congolense TcIL3000_9_2150   arginine kinase
Trypanosoma cruzi TcCLB.507241.30   arginine kinase
Toxoplasma gondii TGME49_231610   ATP:guanido phosphotransferase, C-terminal catalytic domain-containing protein

Essentiality

Tb927.9.6290 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.4560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.4560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.4560 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.4560 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_231610 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma cruzi arginine kinase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Arginine Kinase (2.7.3.3 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.9.6290 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Trypanosoma brucei ( 1 )

Bibliographic References

11 literature references were collected for this gene.

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Gene identifier Tb927.9.6290 (Trypanosoma brucei), arginine kinase
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