Detailed view for Tb11.v5.0435

Basic information

TDR Targets ID: 12650
Trypanosoma brucei, adenylosuccinate synthetase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.1903 | Length (AA): 602 | MW (Da): 66675 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00709   Adenylosuccinate synthetase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005525   GTP binding  
GO:0004019   adenylosuccinate synthase activity  
GO:0000287   magnesium ion binding  
GO:0006164   purine nucleotide biosynthetic process  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
58 599 1dj3 (A) 13 441 27.00 0 1 0.851132 1.11
65 598 5i34 (A) 8 428 32.00 0 1 0.828743 1.09

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127011)

Species Accession Gene Product
Arabidopsis thaliana AT3G57610   adenylosuccinate synthetase
Babesia bovis BBOV_II004950   adenylosuccinate synthetase, putative
Brugia malayi Bm1_50695   adss-prov protein
Candida albicans CaO19.12290   Adenylosuccinate synthetase
Candida albicans CaO19.4827   Adenylosuccinate synthetase
Caenorhabditis elegans CELE_C37H5.6   Protein C37H5.6, isoform B
Dictyostelium discoideum DDB_G0285429   adenylosuccinate synthetase
Drosophila melanogaster Dmel_CG17273   Adenylosuccinate Synthetase
Escherichia coli b4177   adenylosuccinate synthetase
Echinococcus granulosus EgrG_001056900   adenylosuccinate synthetase
Echinococcus multilocularis EmuJ_001056900   adenylosuccinate synthetase
Homo sapiens ENSG00000035687   adenylosuccinate synthase
Homo sapiens ENSG00000185100   adenylosuccinate synthase like 1
Leishmania braziliensis LbrM.13.0970   adenylosuccinate synthetase, putative
Leishmania donovani LdBPK_131090.1   adenylosuccinate synthetase, putative
Leishmania infantum LinJ.13.1090   adenylosuccinate synthetase, putative
Leishmania major LmjF.13.1190   adenylosuccinate synthetase, putative
Leishmania mexicana LmxM.13.1190   adenylosuccinate synthetase, putative
Loa Loa (eye worm) LOAG_01402   adss-prov protein
Mycobacterium leprae ML0280c   Probable adenylosuccinate synthase PurA (IMP--aspartate ligase) (AdSS) (AMPSase)
Mus musculus ENSMUSG00000011148   adenylosuccinate synthetase like 1
Mus musculus ENSMUSG00000015961   adenylosuccinate synthetase, non muscle
Mycobacterium tuberculosis Rv0357c   Probable adenylosuccinate synthetase PurA (imp--aspartate ligase) (ADSS) (ampsase)
Mycobacterium ulcerans MUL_2834   adenylosuccinate synthetase
Neospora caninum NCLIV_038330   adenylosuccinate synthetase, putative
Oryza sativa 4331792   Os03g0174500
Oryza sativa 4333815   Os03g0699300
Onchocerca volvulus OVOC7126   Adenylosuccinate synthetase homolog
Plasmodium berghei PBANKA_1131100   adenylosuccinate synthetase, putative
Plasmodium falciparum PF3D7_1354500   adenylosuccinate synthetase
Plasmodium knowlesi PKNH_1117300   adenylosuccinate synthetase, putative
Plasmodium vivax PVX_114710   adenylosuccinate synthetase, putative
Plasmodium yoelii PY03301   adenylosuccinate synthetase
Saccharomyces cerevisiae YNL220W   adenylosuccinate synthase
Schistosoma japonicum Sjp_0015930   ko:K01939 adenylosuccinate synthase [EC6.3.4.4], putative
Schistosoma mansoni Smp_055990   adenylosuccinate synthetase
Schmidtea mediterranea mk4.028749.00   Adenylosuccinate synthetase
Schmidtea mediterranea mk4.033749.02   Adenylosuccinate synthetase
Schmidtea mediterranea mk4.014784.00  
Schmidtea mediterranea mk4.031257.00   Adenylosuccinate synthetase
Trypanosoma brucei gambiense Tbg972.11.4160   adenylosuccinate synthetase, putative
Trypanosoma brucei Tb11.v5.0435   adenylosuccinate synthetase, putative
Trypanosoma congolense TcIL3000.11.3610   adenylosuccinate synthetase, putative
Trypanosoma cruzi TcCLB.509683.80   adenylosuccinate synthetase, putative
Trypanosoma cruzi TcCLB.508731.60   adenylosuccinate synthetase, putative
Toxoplasma gondii TGME49_279450   adenylosuccinate synthetase, putative
Theileria parva TP04_0033   adenylosuccinate synthase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0273   adenylosuccinate synthetase

Essentiality

Tb11.v5.0435 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu360 Mycobacterium tuberculosis essential nmpdr
Tb11.02.1120 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.1120 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.1120 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.1120 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b4177 Escherichia coli non-essential goodall
CELE_C37H5.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_C37H5.6 Caenorhabditis elegans slow growth wormbase
TGME49_279450 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens adenylosuccinate synthase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0057 0.3589 0
0.0057 0.3589 0

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tbru022237AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tbru022237; Recombinant protein: full-length; Source: T brucei; adenylosuccinate synthetase, putative ;

Bibliographic References

4 literature references were collected for this gene.

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Gene identifier Tb11.v5.0435 (Trypanosoma brucei), adenylosuccinate synthetase, putative
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