Detailed view for Tb927.6.4590

Basic information

TDR Targets ID: 12762
Trypanosoma brucei, glutamyl-tRNA synthetase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.8316 | Length (AA): 607 | MW (Da): 68932 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00749   tRNA synthetases class I (E and Q), catalytic domain
PF03950   tRNA synthetases class I (E and Q), anti-codon binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0004818   glutamate-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0000166   nucleotide binding  
GO:0005524   ATP binding  
GO:0043039   tRNA aminoacylation  
GO:0006424   glutamyl-tRNA aminoacylation  
GO:0006418   tRNA aminoacylation for protein translation  
GO:0006412   translation  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128638)

Species Accession Gene Product
Arabidopsis thaliana AT5G26710   glutamyl/glutaminyl-tRNA synthetase, class Ic
Babesia bovis BBOV_III011840   glutamyl-tRNA synthetase family protein
Candida albicans CaO19_7057   hypothetical protein
Candida albicans CaO19.7057   similar to S. cerevisiae YGL245W
Caenorhabditis elegans CELE_ZC434.5   Protein EARS-1
Cryptosporidium hominis Chro.80098   glutamate--tRNA ligase
Cryptosporidium parvum cgd8_790   glutamate--tRNA ligase
Dictyostelium discoideum DDB_G0287467   glutamate-tRNA ligase
Entamoeba histolytica EHI_155570   glutamyl-tRNA synthetase, putative
Giardia lamblia GL50803_86681   Glutaminyl-tRNA synthetase
Leishmania braziliensis LbrM.30.3280   glutamyl-tRNA synthetase, putative
Leishmania donovani LdBPK_303290.1   glutamyl-tRNA synthetase, putative
Leishmania infantum LinJ.30.3290   glutamyl-tRNA synthetase, putative
Leishmania major LmjF.30.3240   glutamyl-tRNA synthetase, putative
Leishmania mexicana LmxM.29.3240   glutamyl-tRNA synthetase, putative
Neospora caninum NCLIV_024090   glutamyl-tRNA synthetase, putative
Oryza sativa 4348464   Os10g0369000
Oryza sativa 4325167   Os01g0271200
Plasmodium berghei PBANKA_1362000   glutamate--tRNA ligase, putative
Plasmodium falciparum PF3D7_1349200   glutamate--tRNA ligase, putative
Plasmodium knowlesi PKNH_1251700   glutamate--tRNA ligase, putative
Plasmodium vivax PVX_083355   glutamate--tRNA ligase, putative
Plasmodium yoelii PY02891   glutamyl-tRNA synthetase, putative
Saccharomyces cerevisiae YGL245W   glutamate--tRNA ligase GUS1
Trypanosoma brucei gambiense Tbg972.6.4410   glutamyl-tRNA synthetase, putative
Trypanosoma brucei Tb11.v5.0810   glutamyl-tRNA synthetase, putative
Trypanosoma brucei Tb927.6.4590   glutamyl-tRNA synthetase, putative
Trypanosoma congolense TcIL3000_6_4040   glutamyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.511731.60   glutamyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.506831.70   glutamyl-tRNA synthetase, putative
Toxoplasma gondii TGME49_263870   glutamate-tRNA ligase
Theileria parva TP01_1053   glutamyl-tRNA synthetase, putative
Trichomonas vaginalis TVAG_047990   glutaminyl-tRNA synthetase, putative

Essentiality

Tb927.6.4590 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.4590 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.4590 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.4590 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.6.4590 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_ZC434.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_ZC434.5 Caenorhabditis elegans larval lethal wormbase
CELE_ZC434.5 Caenorhabditis elegans slow growth wormbase
YGL245W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1362000 Plasmodium berghei Essential plasmo
TGME49_263870 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Aminoacyl-tRNA Synthetase (6.1.1.17 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.6.4590 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma brucei ( 1 )

Bibliographic References

2 literature references were collected for this gene.

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Gene identifier Tb927.6.4590 (Trypanosoma brucei), glutamyl-tRNA synthetase, putative
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