Detailed view for Tb927.5.3580

Basic information

TDR Targets ID: 14942
Trypanosoma brucei, phosphoglycerate mutase protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.1501 | Length (AA): 185 | MW (Da): 20718 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00300   Histidine phosphatase superfamily (branch 1)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 185 3gp3 (A) 1 204 31.00 0.0000002 1 1.1174 0.55
1 185 4ij5 (A) 1 182 25.00 0 1 1.3914 -0.83
2 185 1h2e (A) 3 184 26.00 0 1 1.40319 -0.77
3 106 5um0 (A) 2 109 38.00 0.000033 0.67 0.754562 0.47
4 185 2a6p (A) 8 179 20.00 0 0.97 1.24018 -0.43

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129378)

Species Accession Gene Product
Arabidopsis thaliana AT5G04120   phosphoglycerate mutase-like protein
Arabidopsis thaliana AT3G50520   phosphoglycerate mutase
Candida albicans CaO19.13477   similar to phosphoglycerate mutases
Candida albicans CaO19.6056   similar to phosphoglycerate mutases
Drosophila melanogaster Dmel_CG7059   CG7059 gene product from transcript CG7059-RA
Drosophila melanogaster Dmel_CG15874   Phosphoglycerate mutase 5-2
Escherichia coli b4395   phosphatase
Leishmania braziliensis LbrM.08.0060   phosphoglycerate mutase protein, putative
Leishmania donovani LdBPK_080060.1   phosphoglycerate mutase protein, putative
Leishmania infantum LinJ.08.0060   phosphoglycerate mutase protein, putative
Leishmania major LmjF.08.0060   phosphoglycerate mutase protein, putative
Leishmania mexicana LmxM.08.0060   phosphoglycerate mutase protein, putative
Mycobacterium leprae ML1452c   PROBABLE PHOSPHOGLYCERATE MUTASE (PHOSPHOGLYCEROMUTASE)
Mycobacterium tuberculosis Rv2228c   Multifunctional protein. Has RNASE H, alpha-ribazole phosphatase, and acid phosphatase activities.
Mycobacterium ulcerans MUL_3691   phosphoglycerate mutase
Neospora caninum NCLIV_034520   Phosphoglycerate/bisphosphoglycerate mutase (EC 5.4.2.1), related
Oryza sativa 4349723   Os11g0138600
Saccharomyces cerevisiae YOR283W   phosphoglycerate mutase
Trypanosoma brucei gambiense Tbg972.5.5000   phosphoglycerate mutase protein, putative
Trypanosoma brucei Tb927.5.3580   phosphoglycerate mutase protein, putative
Trypanosoma congolense TcIL3000_0_40660   phosphoglycerate mutase protein, putative
Trypanosoma congolense TcIL3000_5_4010   phosphoglycerate mutase protein, putative
Trypanosoma cruzi TcCLB.511649.150   phosphoglycerate mutase protein, putative
Trypanosoma cruzi TcCLB.507811.44   phosphoglycerate mutase protein, putative
Toxoplasma gondii TGME49_273030   phosphoglycerate mutase family protein
Trichomonas vaginalis TVAG_165570   phosphoglycerate mutase, putative

Essentiality

Tb927.5.3580 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.3580 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.3580 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.3580 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.5.3580 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b4395 Escherichia coli non-essential goodall
TGME49_273030 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 15955817
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Phosphoglycerate Mutase (5.4.2.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 1 )

Reagent availability

  • Tbru015950AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tbru015950; Recombinant protein: full-length; Source: T brucei; phosphoglycerate mutase protein, putative ;
  • Reagent:
  • Target Type Source Notes
    Tb927.5.3580 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Trypanosoma brucei ( 1 )

Bibliographic References

4 literature references were collected for this gene.

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Gene identifier Tb927.5.3580 (Trypanosoma brucei), phosphoglycerate mutase protein, putative
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