Detailed view for Tb927.5.1260

Basic information

TDR Targets ID: 16068
Trypanosoma brucei, Sulfate transporter N-terminal domain with GLY motif/Sulfate transporter family, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.8421 | Length (AA): 573 | MW (Da): 62563 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 11

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00916   Sulfate permease family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0015116   sulfate transmembrane transporter activity  
GO:0008271   secondary active sulfate transmembrane transporter activity  
GO:0005215   transporter activity  
GO:0008272   sulfate transport  
GO:0006810   transport  
GO:0055085   transmembrane transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
31 438 5da0 (A) 16 385 25.00 0 1 0.897542 0.39
42 549 5da0 (A) 18 474 20.00 0 1 0.987462 0.59
44 108 1zfn (B) 128 206 37.00 0.05 0.47 0.259938 0.65
471 550 5ezb (A) 541 696 26.00 0 0.48 0.431516 -0.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129533)

Species Accession Gene Product
Babesia bovis BBOV_II006030   sulfate transporter, putative
Chlamydia trachomatis CT_856   sulfate transporter
Escherichia coli b1206   C4-dicarboxylic acid transporter
Mycobacterium leprae ML2279c   Probable transmembrane transport protein
Mycobacterium tuberculosis Rv1707   Probable conserved transmembrane protein
Mycobacterium tuberculosis Rv3273   Probable transmembrane carbonic anhydrase (carbonate dehydratase) (carbonic dehydratase)
Mycobacterium ulcerans MUL_5080   transmembrane carbonic anhydrase, SulP
Mycobacterium ulcerans MUL_0666   transmembrane carbonic anhydrase, SulP
Mycobacterium ulcerans MUL_0321   integral membrane transporter
Neospora caninum NCLIV_019650   hypothetical protein
Plasmodium berghei PBANKA_1334400   inorganic anion antiporter, putative
Plasmodium falciparum PF3D7_1471200   inorganic anion exchanger, inorganic anion antiporter
Plasmodium knowlesi PKNH_1209800   sulfate transporter, putative
Plasmodium vivax PVX_116910   sulfate transporter, putative
Plasmodium yoelii PY07224   sulfate permease family protein
Trypanosoma brucei gambiense Tbg972.5.1740   hypothetical protein, conserved
Trypanosoma brucei Tb927.5.1260   Sulfate transporter N-terminal domain with GLY motif/Sulfate transporter family, putative
Trypanosoma congolense TcIL3000_5_1250   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_0_42680   Sulfate transporter N-terminal domain with GLY motif/Sulfate transporter family, putative
Trypanosoma cruzi TcCLB.509601.110   Sulfate transporter N-terminal domain with GLY motif/Sulfate transporter family, putative
Trypanosoma cruzi TcCLB.509767.110   Sulfate transporter N-terminal domain with GLY motif/Sulfate transporter family, putative
Toxoplasma gondii TGME49_280500   inorganic anion transporter, sulfate permease (SulP) family protein
Theileria parva TP02_0554   sulfate transporter, putative

Essentiality

Tb927.5.1260 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.1260 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.1260 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.1260 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.5.1260 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b1206 Escherichia coli non-essential goodall
TGME49_280500 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mycobacterium tuberculosis Probable transmembrane carbonic anhydrase (carbonate dehydratase) (carbonic dehydratase) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.5.1260 (Trypanosoma brucei), Sulfate transporter N-terminal domain with GLY motif/Sulfate transporter family, putative
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