Detailed view for Tb927.9.3440

Basic information

TDR Targets ID: 16088
Trypanosoma brucei, cyclophilin type peptidyl-prolyl cis-trans isomerase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.2205 | Length (AA): 318 | MW (Da): 35780 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00160   Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0000413   protein peptidyl-prolyl isomerization  
GO:0003755   peptidyl-prolyl cis-trans isomerase activity  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_133785)

Species Accession Gene Product
Echinococcus granulosus EgrG_000477000   peptidyl prolyl cis trans isomerase 6
Echinococcus multilocularis EmuJ_000477000   peptidyl prolyl cis trans isomerase 6
Homo sapiens ENSG00000185250   peptidylprolyl isomerase (cyclophilin)-like 6
Leishmania braziliensis LbrM.01.0250   cyclophilin 12, putative
Leishmania donovani LdBPK_010220.1   cyclophilin type peptidyl-prolyl cis-trans isomerase, putative
Leishmania infantum LinJ.01.0220   cyclophilin 12, putative
Leishmania major LmjF.01.0220   cyclophilin 12, putative
Leishmania mexicana LmxM.01.0220   cyclophilin type peptidyl-prolyl cis-trans isomerase, putative
Mus musculus ENSMUSG00000078451   peptidylprolyl isomerase (cyclophilin)-like 6
Schistosoma japonicum Sjp_0070260   ko:K01802 peptidylprolyl isomerase [EC5.2.1.8], putative
Schistosoma mansoni Smp_054330   cyclophilin
Schmidtea mediterranea mk4.000351.04   Peptidyl-prolyl cis-trans isomerase-like 6
Trypanosoma brucei gambiense Tbg972.9.1740   cyclophilin type peptidyl-prolyl cis-trans isomerase, putative
Trypanosoma brucei Tb927.9.3440   cyclophilin type peptidyl-prolyl cis-trans isomerase, putative
Trypanosoma congolense TcIL3000_9_1010   cyclophilin type peptidyl-prolyl cis-trans isomerase, putative
Trypanosoma cruzi TcCLB.504797.100   rotamase, putative
Trypanosoma cruzi TcCLB.511577.40   35 kDa cyclophilin, putative
Toxoplasma gondii TGME49_204325   hypothetical protein

Essentiality

Tb927.9.3440 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.2070 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.160.2070 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.160.2070 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.160.2070 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Cyclophilin A 164 aa 36.0% 175 aa Compounds References
Plasmodium falciparum peptidyl-prolyl cis-trans isomerase 171 aa 40.8% 169 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for FK-Binding Protein Peptidyl-Prolyl Isomerase Activity (5.2.1.8 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

9 literature references were collected for this gene.

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Gene identifier Tb927.9.3440 (Trypanosoma brucei), cyclophilin type peptidyl-prolyl cis-trans isomerase, putative
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