Detailed view for Tb927.9.12570

Basic information

TDR Targets ID: 17364
Trypanosoma brucei, glycerol kinase, glycosomal
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.3009 | Length (AA): 512 | MW (Da): 56366 | Paralog Number: 4

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00370   FGGY family of carbohydrate kinases, N-terminal domain
PF02782   FGGY family of carbohydrate kinases, C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016773   phosphotransferase activity, alcohol group as acceptor  
GO:0004370   glycerol kinase activity  
GO:0006072   glycerol-3-phosphate metabolic process  
GO:0005975   carbohydrate metabolic process  

Metabolic Pathways

Glycerolipid metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 511 3wxl (A) 1 511 99.99 0 1 2.24365 -1.52

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3WXI:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3WXJ:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3WXK:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3WXL:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 5AZI:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 5AZJ:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 5GN5:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 5GN6:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126746)

Species Accession Gene Product
Arabidopsis thaliana AT1G80460   glycerol kinase
Babesia bovis BBOV_I004170   glycerol kinase, putative
Brugia malayi Bm1_33005   FGGY family of carbohydrate kinases, N-terminal domain containing protein
Candida albicans CaO19.558   likely carbohydrate kinase similar to S. cerevisiae GUT1 (YHL032C) glycerol kinase
Candida albicans CaO19.8193   likely carbohydrate kinase similar to S. cerevisiae GUT1 (YHL032C) glycerol kinase
Caenorhabditis elegans CELE_R11F4.1   Protein R11F4.1
Caenorhabditis elegans CELE_C55B6.1   Protein C55B6.1, isoform B
Dictyostelium discoideum DDB_G0280371   hypothetical protein
Dictyostelium discoideum DDB_G0278551   hypothetical protein
Drosophila melanogaster Dmel_CG1271   CG1271 gene product from transcript CG1271-RC
Drosophila melanogaster Dmel_CG7995   Glycerol kinase
Drosophila melanogaster Dmel_CG18374   Glycerol kinase
Escherichia coli b3926   glycerol kinase
Echinococcus granulosus EgrG_000180000   glycerol kinase
Echinococcus granulosus EgrG_000775900   glycerol kinase 5
Entamoeba histolytica EHI_164850   glycerol kinase, putative
Echinococcus multilocularis EmuJ_000180000   glycerol kinase
Echinococcus multilocularis EmuJ_000775900   glycerol kinase 5
Giardia lamblia GL50803_8173   Glycerol kinase
Homo sapiens ENSG00000196475   glycerol kinase 2
Homo sapiens ENSG00000198814   glycerol kinase
Homo sapiens ENSG00000175066   glycerol kinase 5 (putative)
Leishmania braziliensis LbrM.34.2990   glycerol kinase, glycosomal, putative
Leishmania donovani LdBPK_353130.1   glycerol kinase, glycosomal, putative
Leishmania infantum LinJ.35.3130   glycerol kinase, glycosomal, putative
Leishmania major LmjF.35.3080   glycerol kinase, glycosomal, putative
Leishmania mexicana LmxM.34.3080   glycerol kinase, glycosomal, putative
Loa Loa (eye worm) LOAG_11455   hypothetical protein
Loa Loa (eye worm) LOAG_05489   hypothetical protein
Mycobacterium leprae ML2314c   PROBABLE GLYCEROL KINASE GLPK (ATP:GLYCEROL 3-PHOSPHOTRANSFERASE)(GLYCEROKINASE) (GK)
Mus musculus ENSMUSG00000025059   glycerol kinase
Mus musculus ENSMUSG00000050553   glycerol kinase 2
Mus musculus ENSMUSG00000053624   glycerol kinase-like 1
Mus musculus ENSMUSG00000041440   glycerol kinase 5 (putative)
Mycobacterium tuberculosis Rv3696c   Probable glycerol kinase GlpK (ATP:glycerol 3-phosphotransferase) (glycerokinase) (GK)
Mycobacterium ulcerans MUL_4281   glycerol kinase
Oryza sativa 4337206   Os04g0647800
Onchocerca volvulus OVOC2966   Putative glycerol kinase 5
Onchocerca volvulus OVOC11223   Putative glycerol kinase
Plasmodium berghei PBANKA_1364300   glycerol kinase, putative
Plasmodium falciparum PF3D7_1351600   glycerol kinase
Plasmodium knowlesi PKNH_1249400   glycerol kinase, putative
Plasmodium vivax PVX_083470   glycerol kinase, putative
Plasmodium yoelii PY00935   putative glycerol kinase
Saccharomyces cerevisiae YHL032C   glycerol kinase
Schistosoma japonicum Sjp_0060360   ko:K00864 glycerol kinase [EC2.7.1.30], putative
Schistosoma japonicum Sjp_0054690   ko:K00864 glycerol kinase [EC2.7.1.30], putative
Schistosoma japonicum Sjp_0308260   Putative glycerol kinase 5, putative
Schistosoma japonicum Sjp_0093890   Glycerol kinase, putative
Schistosoma mansoni Smp_124440   glycerol kinase
Schistosoma mansoni Smp_141770   glycerol kinase
Schmidtea mediterranea mk4.001024.05   Putative glycerol kinase 5
Schmidtea mediterranea mk4.009692.00   Probable glycerol kinase
Schmidtea mediterranea mk4.050118.00  
Schmidtea mediterranea mk4.005092.03   Probable glycerol kinase
Schmidtea mediterranea mk4.002372.04   Probable glycerol kinase
Schmidtea mediterranea mk4.025148.04  
Schmidtea mediterranea mk4.002508.00   Probable glycerol kinase
Trypanosoma brucei gambiense Tbg972.9.7700   glycerol kinase, glycosomal
Trypanosoma brucei gambiense Tbg972.9.7710   glycerol kinase, glycosomal
Trypanosoma brucei Tb927.9.12610   glycerol kinase, glycosomal
Trypanosoma brucei Tb927.9.12550   glycerol kinase, glycosomal
Trypanosoma brucei Tb927.9.12590   glycerol kinase, glycosomal
Trypanosoma brucei Tb427tmp.211.3570   glycerol kinase, glycosomal
Trypanosoma brucei Tb927.9.12570   glycerol kinase, glycosomal
Trypanosoma congolense TcIL3000_0_55170   glycerol kinase, glycosomal
Trypanosoma cruzi TcCLB.510661.60   glycerol kinase, glycosomal, putative
Theileria parva TP02_0725   glycerol kinase, putative
Trichomonas vaginalis TVAG_353000   xylulose kinase, putative

Essentiality

Tb927.9.12570 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.3540 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.3540 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.3540 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.3540 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3926 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 3 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.9.12570 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma brucei ( 1 )

Bibliographic References

11 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.9.12570 (Trypanosoma brucei), glycerol kinase, glycosomal
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