Detailed view for PF3D7_1355500

Basic information

TDR Targets ID: 189
Plasmodium falciparum, serine/threonine protein phosphatase 5
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 4.8117 | Length (AA): 658 | MW (Da): 76889 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00149   Calcineurin-like phosphoesterase
PF08321   PPP5 TPR repeat region
PF13181   Tetratricopeptide repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0008287   protein serine/threonine phosphatase complex  
GO:0046872   metal ion binding  
GO:0016787   hydrolase activity  
GO:0005515   protein binding  
GO:0005488   binding  
GO:0004722   protein serine/threonine phosphatase activity  
GO:0004721   phosphoprotein phosphatase activity  
GO:0006470   protein amino acid dephosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
132 273 1na0 (A) 7 125 23.00 0 0.34 0.27 -0.11
134 292 2fo7 (A) 1 136 27.00 0 1 0.42 0.03
186 656 1wao (1) 24 495 43.00 0 1 1.35 -1.36
364 628 1aui (A) 55 327 39.00 0 1 0.99 -1.83
14 136 1l8q (A) 223 349 32.00 0.14 0.47 0.40583 -0.1
23 101 3gw4 (A) 93 171 9.00 0.43 0.01 0.301461 -1.17
192 656 4ja7 (A) 30 495 44.00 0 1 1.35859 -1.4
226 302 2vyi (A) 126 205 38.00 0.00015 0.95 0.632921 -0.79
346 651 3icf (A) 190 507 45.00 0 1 1.15495 -1.88

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 48 hs, Oocyst, Ring, Male Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 40 hs, Female Gametocyte. Otto TD Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Sporozoite. Zanghi G
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_128254)

Species Accession Gene Product
Arabidopsis thaliana AT2G42810   serine/threonine-protein phosphatase 5
Babesia bovis BBOV_IV000160   serine/threonine protein phosphatase 5
Brugia malayi Bm1_15955   serine/threonine protein phosphatase 5
Candida albicans CaO19.1673   potential calcineurin-like serine/threonine phosphatase similar to S. cerevisiae PPT1 (YGR123C)
Candida albicans CaO19.9242   potential calcineurin-like Serine/Threonine phosphatase similar to S. cerevisiae PPT1 (YGR123C)
Caenorhabditis elegans CELE_Y39B6A.2   Protein PPH-5, isoform B
Cryptosporidium hominis Chro.20310   phosphoprotein phosphatase -related
Cryptosporidium parvum cgd2_2960   phosphoprotein phosphatase related, putative
Dictyostelium discoideum DDB_G0283157   hypothetical protein
Drosophila melanogaster Dmel_CG8402   Protein phosphatase D3
Echinococcus granulosus EgrG_001102500   protein phosphatase 5
Entamoeba histolytica EHI_162200   protein phosphatase, putative
Echinococcus multilocularis EmuJ_001102500   protein phosphatase 5
Homo sapiens ENSG00000011485   protein phosphatase 5, catalytic subunit
Leishmania braziliensis LbrM.18.0170   serine/threonine protein phosphatase type 5, putative
Leishmania donovani LdBPK_180150.1   serine/threonine protein phosphatase type 5, putative
Leishmania infantum LinJ.18.0150   serine/threonine protein phosphatase type 5, putative
Leishmania major LmjF.18.0150   serine/threonine protein phosphatase type 5, putative
Leishmania mexicana LmxM.18.0150   serine/threonine protein phosphatase type 5, putative
Loa Loa (eye worm) LOAG_03443   serine/threonine protein phosphatase 5
Loa Loa (eye worm) LOAG_12431   protein phosphatase 5
Mus musculus ENSMUSG00000003099   protein phosphatase 5, catalytic subunit
Neospora caninum NCLIV_055800   Serine/threonine protein phosphatase 5 (EC 3.1.3.16), related
Oryza sativa 4338070   Os05g0204900
Plasmodium berghei PBANKA_1131900   serine/threonine protein phosphatase 5
Plasmodium falciparum PF3D7_1355500   serine/threonine protein phosphatase 5
Plasmodium knowlesi PKNH_1116000   serine/threonine protein phosphatase 5, putative
Plasmodium vivax PVX_114750   serine/threonine protein phosphatase 5, putative
Plasmodium yoelii PY04697   serine/threonine protein phosphatase PP5
Saccharomyces cerevisiae YGR123C   Ppt1p
Schistosoma japonicum Sjp_0010280   ko:K04460 protein phosphatase 5, putative
Schistosoma mansoni Smp_090860   protein phosphatase-5
Schmidtea mediterranea mk4.013727.00  
Schmidtea mediterranea mk4.055730.00  
Trypanosoma brucei gambiense Tbg972.10.16800   serine/threonine protein phosphatase type 5, putative
Trypanosoma brucei Tb927.10.13670   serine/threonine protein phosphatase 5
Trypanosoma congolense TcIL3000_10_13810   serine/threonine protein phosphatase 5
Trypanosoma cruzi TcCLB.507993.190   serine/threonine protein phosphatase type 5, putative
Trypanosoma cruzi TcCLB.511277.630   serine/threonine protein phosphatase type 5, putative
Toxoplasma gondii TGME49_312200   serine/threonine protein phosphatase
Theileria parva TP02_0944   serine/threonine protein phosphatase, putative
Trichomonas vaginalis TVAG_077920   protein phosphatase-5, putative
Trichomonas vaginalis TVAG_331100   protein phosphatase-1, putative
Trichomonas vaginalis TVAG_203890   protein phosphatase-1, putative

Essentiality

PF3D7_1355500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.13670 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.13670 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.13670 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.13670 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y39B6A.2 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_1131900 Plasmodium berghei Dispensable plasmo
TGME49_312200 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens protein phosphatase 5, catalytic subunit Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Calcineurin (3.1.3.16 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

  • Reagent:
  • Target Type Source Notes
    PF3D7_1355500 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Plasmodium falciparum ( 1 )

Bibliographic References

25 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_1355500 (Plasmodium falciparum), serine/threonine protein phosphatase 5
Title for this comment
Comment