Detailed view for Tb927.9.7470

Basic information

TDR Targets ID: 18991
Trypanosoma brucei, purine nucleoside transporter
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.9218 | Length (AA): 462 | MW (Da): 50676 | Paralog Number: 7

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 10

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01733   Nucleoside transporter

Gene Ontology

Mouse over links to read term descriptions.
GO:1901642   GO:nucleoside transmembrane transport  

GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0005337   nucleoside transmembrane transporter activity  
GO:0015858   nucleoside transport  
GO:0006810   transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
305 460 4n7w (A) 11 140 28.00 0 0.03 0.154062 2.5

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_133631)

Species Accession Gene Product
Cryptosporidium hominis Chro.20039   CG31911-PA
Cryptosporidium parvum cgd2_310   nucleoside transporter, 10 transmembrane domain domain, nucleoside binding domain
Entamoeba histolytica EHI_110730   nucleoside transporter, putative
Leishmania braziliensis LbrM.35.2150   nucleoside transporter 1, putative
Leishmania donovani LdBPK_362040.1   inosine-guanosine transporter
Leishmania infantum LinJ.36.2040   inosine-guanosine transporter
Leishmania major LmjF.36.1940   inosine-guanosine transporter;with=GeneDB:LinJ36_V3.2040
Leishmania mexicana LmxM.36.1940   nucleoside transporter 1, putative
Trypanosoma brucei gambiense Tbg.972.2.4320   adenosine transporter 2, putative
Trypanosoma brucei gambiense Tbg.972.2.4300   adenosine transporter 2
Trypanosoma brucei gambiense Tbg972.9.4060   adenosine transporter, putative
Trypanosoma brucei gambiense Tbg.972.2.4350   adenosine transporter 2, putative
Trypanosoma brucei Tb927.9.7470   purine nucleoside transporter
Trypanosoma brucei Tb927.6.220   purine nucleoside transporter
Trypanosoma brucei Tb927.2.6150   adenosine transporter 2
Trypanosoma brucei Tb927.2.6200   adenosine transporter 2, putative
Trypanosoma brucei Tb927.2.6220   adenosine transporter 2, putative
Trypanosoma brucei Tb927.2.6240   adenosine transporter 2
Trypanosoma brucei Tb927.2.6280   adenosine transporter 2, putative
Trypanosoma brucei Tb927.2.6320   adenosine transporter 2, putative
Trypanosoma congolense TcIL3000_9_2500   purine nucleoside transporter, putative
Trypanosoma cruzi TcCLB.508645.70   nucleoside transporter 1, putative
Trypanosoma cruzi TcCLB.508645.40   nucleoside transporter 1, putative

Essentiality

Tb927.9.7470 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.2.6150 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.6150 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.2.6150 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.2.6150 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.2.6200 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.6200 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.2.6200 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.2.6200 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.6.220 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.220 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.220 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.220 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.2.6240 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.6240 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.2.6240 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.2.6240 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.2.6220 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.6220 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.2.6220 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.2.6220 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb09.160.5480 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.5480 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.5480 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.5480 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma brucei adenosine transporter 2 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Equilibrative nucleoside transporter 1 457 aa 24.0% 430 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.9.7470 (Trypanosoma brucei), purine nucleoside transporter
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