Detailed view for Tb927.9.3400

Basic information

TDR Targets ID: 20447
Trypanosoma brucei, endo-beta-N-acetylglucosaminidase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.6686 | Length (AA): 1051 | MW (Da): 115905 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03644   Glycosyl hydrolase family 85
PF05903   PPPDE putative peptidase domain
PF09409   PUB domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016798   hydrolase activity, acting on glycosyl bonds  
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0005737   cytoplasm  
GO:0033925   mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity  

Metabolic Pathways

Other glycan degradation (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130163)

Species Accession Gene Product
Arabidopsis thaliana AT5G05460   beta-endo-N-acetyglucosaminidase
Arabidopsis thaliana AT3G11040   glycosyl hydrolase family 85 protein
Brugia malayi Bm1_17595   Glycosyl hydrolase family 85 protein
Brugia malayi Bm1_00495   hypothetical protein
Caenorhabditis elegans CELE_F01F1.10   Protein ENG-1, isoform C
Dictyostelium discoideum DDB_G0268520   hypothetical protein
Drosophila melanogaster Dmel_CG5613   CG5613 gene product from transcript CG5613-RA
Echinococcus granulosus EgrG_000434910   endo beta n-acetylglucosaminidase
Echinococcus granulosus EgrG_000434920   mitochondrial ribosomal protein L17
Echinococcus multilocularis EmuJ_000434920   mitochondrial ribosomal protein L17
Echinococcus multilocularis EmuJ_000434910   endo beta n-acetylglucosaminidase
Homo sapiens 64772   endo-beta-N-acetylglucosaminidase
Leishmania braziliensis LbrM.33.2550   glycosyl hydrolase-like protein
Leishmania donovani LdBPK_332390.1   glycosyl hydrolase-like protein
Leishmania infantum LinJ.33.2390   glycosyl hydrolase-like protein
Leishmania major LmjF.33.2260   glycosyl hydrolase-like protein
Leishmania mexicana LmxM.32.2260   glycosyl hydrolase-like protein
Loa Loa (eye worm) LOAG_09591   glycosyl hydrolase family 85 protein
Mus musculus ENSMUSG00000033857   endo-beta-N-acetylglucosaminidase
Oryza sativa 4338487   Os05g0346500
Schistosoma japonicum Sjp_0316890   expressed protein
Schistosoma japonicum Sjp_0311470   expressed protein
Schistosoma japonicum Sjp_0009440   ko:K01227 mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase [EC3.2.1.96], putative
Schistosoma mansoni Smp_174990   endo beta n-acetylglucosaminidase
Trypanosoma brucei gambiense Tbg972.9.1720   endo-beta-N-acetylglucosaminidase, putative
Trypanosoma brucei Tb927.9.3400   endo-beta-N-acetylglucosaminidase, putative
Trypanosoma congolense TcIL3000_9_990   endo-beta-N-acetylglucosaminidase, putative
Trypanosoma cruzi TcCLB.511577.50   endo-beta-N-acetylglucosaminidase, putative
Trypanosoma cruzi TcCLB.504797.80   endo-beta-N-acetylglucosaminidase, putative

Essentiality

Tb927.9.3400 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.2050 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.2050 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.160.2050 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.2050 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens endo-beta-N-acetylglucosaminidase Compounds References
Arthrobacter protophormiae Endo-beta-N-acetylglucosaminidase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0343 1 1
0.0232 0.5 0.5
0.0343 1 1
0.016 0.2871 1
0.016 0.2871 1
0.0153 0.2871 1
0.0343 1 1
0.0153 0.2871 1
0.0172 0.6205 0.5
0.0232 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

12 literature references were collected for this gene.

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Gene identifier Tb927.9.3400 (Trypanosoma brucei), endo-beta-N-acetylglucosaminidase, putative
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