Detailed view for Tb927.9.3280

Basic information

TDR Targets ID: 20468
Trypanosoma brucei, exopolyphosphatase
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.2985 | Length (AA): 383 | MW (Da): 42865 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01368   DHH family
PF02833   DHHA2 domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0006797   polyphosphate metabolic process  
GO:0005575   cellular_component  
GO:0004309   exopolyphosphatase activity  
GO:0005737   cytoplasm  
GO:0030145   manganese ion binding  
GO:0016787   hydrolase activity  
GO:0016462   pyrophosphatase activity  

Metabolic Pathways

Purine metabolism (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 381 2qb7 (A) 6 396 22.00 0 1 1.24006 -0.18
5 380 2qb7 (A) 8 395 25.00 0 1 1.22242 -0.28
13 319 2qb7 (A) 20 339 28.00 0 1 1.00527 -0.01
25 223 4rpa (A) 6 186 25.00 0.01 1 0.727282 -0.45

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129543)

Species Accession Gene Product
Candida albicans CaO19.11588   exopolyphosphatase
Candida albicans CaO19.4107   exopolyphosphatase
Drosophila melanogaster Dmel_CG3461   prune
Echinococcus granulosus EgrG_001064100   protein prune
Homo sapiens ENSG00000143363   prune exopolyphosphatase
Leishmania braziliensis LbrM.01.0340   acidocalcisomal exopolyphosphatase, putative
Leishmania donovani LdBPK_010310.1   acidocalcisomal exopolyphosphatase, putative
Leishmania infantum LinJ.01.0310   acidocalcisomal exopolyphosphatase, putative
Leishmania major LmjF.01.0310   acidocalcisomal exopolyphosphatase, putative
Leishmania mexicana LmxM.01.0310   acidocalcisomal exopolyphosphatase, putative
Mus musculus ensembl-mmu:ENSMUSG00000015711   prune homolog (Drosophila)
Neospora caninum NCLIV_026620   hypothetical protein
Saccharomyces cerevisiae YHR201C   Ppx1p
Schistosoma mansoni Smp_173050.1   hypothetical protein
Schistosoma mansoni Smp_173050.2   hypothetical protein
Trypanosoma brucei gambiense Tbg972.9.1630   acidocalcisomal exopolyphosphatase, putative
Trypanosoma brucei Tb927.9.3280   exopolyphosphatase
Trypanosoma congolense TcIL3000_0_32900   acidocalcisomal exopolyphosphatase
Trypanosoma cruzi TcCLB.511577.110   exopolyphosphatase
Trypanosoma cruzi TcCLB.504797.10   acidocalcisomal exopolyphosphatase, putative
Toxoplasma gondii TGME49_260430   hypothetical protein

Essentiality

Tb927.9.3280 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.1950 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.1950 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.1950 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.1950 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_260430 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens prune exopolyphosphatase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0119 1 1
0.0116 1 0.5
0.0109 0.3278 1
0.0114 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

4 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.9.3280 (Trypanosoma brucei), exopolyphosphatase
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