Detailed view for PF3D7_1359100

Basic information

TDR Targets ID: 208
Plasmodium falciparum, riboflavin kinase / FAD synthase family protein, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.4736 | Length (AA): 707 | MW (Da): 83784 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01687   Riboflavin kinase

Gene Ontology

Mouse over links to read term descriptions.
GO:0008531   riboflavin kinase activity  
GO:0009231   riboflavin biosynthetic process  

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
218 510 2aqw (A) 22 319 23.00 0.0000014 0.45 0.58 -0.11
565 697 1n08 (A) 23 162 38.00 0 1 0.74 -1.4
566 700 1nb0 (A) 13 155 41.00 0 1 0.65 -1.24
134 303 4u5a (C) 47 238 19.00 0.24 0.19 0.404653 -0.37
393 704 2x0k (A) 3 335 18.00 0 1 0.530001 0.82
563 703 1n05 (A) 21 162 32.00 0.0000000002 1 0.468634 -0.11
570 690 1n08 (A) 28 147 43.00 0 1 0.482246 0.56
134 304 4u5a (C) 47 238 19.00 0.047 0.08 0.41739 -0.37
556 698 1n05 (A) 19 162 32.00 0.0000000013 1 0.520504 -0.05
565 685 1n08 (A) 28 147 43.00 0 1 0.483065 0.56

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 48 hs, Oocyst, Sporozoite, Female Gametocyte, Male Gametocyte. Otto TD Zanghi G Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_127161)

Species Accession Gene Product
Arabidopsis thaliana AT4G21470   bifunctional riboflavin kinase/FMN hydrolase
Arabidopsis thaliana AT5G08340   riboflavin kinaseFAD 2
Arabidopsis thaliana AT5G23330   FAD synthetase RibF1
Babesia bovis BBOV_I003450   riboflavin kinase / FAD synthetase domain containing protein
Brugia malayi Bm1_08880   riboflavin kinase
Candida albicans CaO19.11851   similar to FMN, a Riboflavin kinase
Candida albicans CaO19.4373   similar to FMN, a Riboflavin kinase
Caenorhabditis elegans CELE_R10H10.6   Protein R10H10.6
Chlamydia trachomatis CT_093   riboflavin kinase/FAD synthase
Drosophila melanogaster Dmel_CG2846   CG2846 gene product from transcript CG2846-RA
Escherichia coli b0025   bifunctional riboflavin kinase/FAD synthetase
Echinococcus granulosus EgrG_001152500   riboflavin kinase
Entamoeba histolytica EHI_033240   Riboflavin kinase / FAD synthetase, putative
Echinococcus multilocularis EmuJ_001152500   riboflavin kinase
Giardia lamblia GL50803_9698   Riboflavin kinase, FMN adenylyltransferase
Homo sapiens ENSG00000135002   riboflavin kinase
Leishmania braziliensis LbrM.34.3070   riboflavin kinase/fmn adenylyltransferase-like protein
Leishmania donovani LdBPK_353210.1   riboflavin kinase/fmn adenylyltransferase-like protein
Leishmania infantum LinJ.35.3210   riboflavin kinase/fmn adenylyltransferase-like protein
Leishmania major LmjF.35.3160   riboflavin kinase/fmn adenylyltransferase-like protein
Leishmania mexicana LmxM.34.3160   riboflavin kinase/fmn adenylyltransferase-like protein
Loa Loa (eye worm) LOAG_14151   hypothetical protein
Loa Loa (eye worm) LOAG_08840   riboflavin kinase
Mycobacterium leprae ML0852   PROBABLE BIFUNCTIONAL FAD SYNTHETASE/RIBOFLAVIN BIOSYNTHESIS PROTEIN RIBF: RIBOFLAVIN KINASE (FLAVOKINASE) + FMN ADENYLYLTRANSFE
Mus musculus ENSMUSG00000024712   riboflavin kinase
Mycobacterium tuberculosis Rv2786c   Probable bifunctional FAD synthetase/riboflavin biosynthesis protein RibF: riboflavin kinase (flavokinase) + FMN adenylyltransfe
Mycobacterium ulcerans MUL_2146   bifunctional riboflavin kinase/FMN adenylyltransferase
Neospora caninum NCLIV_059550   Riboflavin kinase / FAD synthetase domain containing protein (EC 2.7.1.26), related
Oryza sativa 4348281   Os10g0209300
Oryza sativa 4348834   Os10g0464400
Oryza sativa 4334460   Os03g0801700
Onchocerca volvulus OVOC3960  
Plasmodium berghei PBANKA_1135400   riboflavin kinase / FAD synthase family protein, putative
Plasmodium falciparum PF3D7_1359100   riboflavin kinase / FAD synthase family protein, putative
Plasmodium knowlesi PKNH_1112200   riboflavin kinase / FAD synthase family protein, putative
Plasmodium vivax PVX_114925   hypothetical protein, conserved
Plasmodium yoelii PY00264   Riboflavin kinase / FAD synthetase, putative
Saccharomyces cerevisiae YDR236C   riboflavin kinase
Schistosoma japonicum Sjp_0086090   ko:K00861 riboflavin kinase [EC2.7.1.26], putative
Schistosoma mansoni Smp_013150   riboflavin kinase/fmn adenylyltransferase
Schmidtea mediterranea mk4.000508.03   Riboflavin kinase
Trypanosoma brucei gambiense Tbg972.9.7600   riboflavin kinase, putative
Trypanosoma brucei Tb927.9.12430   riboflavin kinase, putative
Trypanosoma cruzi TcCLB.510661.174   riboflavin kinase, putative
Trypanosoma cruzi TcCLB.510741.80   riboflavin kinase, putative
Toxoplasma gondii TGME49_216740   riboflavin kinase
Treponema pallidum TP0888   riboflavin kinase/FMN adenylyltransferase (ribF)
Theileria parva TP01_0503   hypothetical protein
Trichomonas vaginalis TVAG_409820   riboflavin kinase/fmn adenylyltransferase, putative
Trichomonas vaginalis TVAG_135720   riboflavin kinase/fmn adenylyltransferase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0416   FAD synthase

Essentiality

PF3D7_1359100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.3420 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.3420 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.3420 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.3420 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0025 Escherichia coli essential goodall
YDR236C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1135400 Plasmodium berghei Essential plasmo
TGME49_216740 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_1359100 (Plasmodium falciparum), riboflavin kinase / FAD synthase family protein, putative
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