Detailed view for LmjF.36.1260

Basic information

TDR Targets ID: 21366
Leishmania major, fructose-1,6-bisphosphate aldolase
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.7066 | Length (AA): 371 | MW (Da): 40828 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00274   Fructose-bisphosphate aldolase class-I

Gene Ontology

Mouse over links to read term descriptions.
GO:0004332   fructose-bisphosphate aldolase activity  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  
GO:0006096   glycolysis  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 357 1epx (A) 1 357 96.00 0 1 2.21 -1.96
9 371 1zai (A) 2 363 48.00 0 1 1.7 -1.62
1 358 2qap (A) 1 358 96.00 0 1 2.17876 -1.63

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 1EPX:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2QAP:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2QDG:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2QDH:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127143)

Species Accession Gene Product
Arabidopsis thaliana AT2G01140   fructose-bisphosphate aldolase 3
Arabidopsis thaliana AT4G26520   fructose-bisphosphate aldolase 7
Arabidopsis thaliana AT4G38970   fructose-bisphosphate aldolase 2
Arabidopsis thaliana AT5G03690   fructose-bisphosphate aldolase 4
Arabidopsis thaliana AT2G36460   fructose-bisphosphate aldolase 6
Arabidopsis thaliana AT3G52930   fructose-bisphosphate aldolase
Arabidopsis thaliana AT4G26530   fructose-bisphosphate aldolase 5
Babesia bovis BBOV_IV000790   fructose-1,6-bisphosphate aldolase
Brugia malayi Bm1_15350   fructose-bisphosphate aldolase 1
Brugia malayi Bm1_09045   fructose-bisphosphate aldolase 2
Caenorhabditis elegans CELE_F01F1.12   Protein ALDO-2, isoform B
Caenorhabditis elegans CELE_T05D4.1   Protein ALDO-1
Cryptosporidium hominis Chro.10335   fructose-1,6-bisphosphate aldolase
Cryptosporidium parvum cgd1_3020   fructose-1,6-bisphosphate aldolase
Dictyostelium discoideum DDB_G0274375   fructose 1,6-bisphosphate aldolase
Drosophila melanogaster Dmel_CG6058   Aldolase
Echinococcus granulosus EgrG_000905600   fructose 16 bisphosphate aldolase
Echinococcus granulosus EgrG_000980100   fructose 16 bisphosphate aldolase
Echinococcus granulosus EgrG_000993700   fructose bisphosphate aldolase class I
Echinococcus granulosus EgrG_000993800   fructose 16 bisphosphate aldolase
Echinococcus multilocularis EmuJ_000993800   fructose 1,6 bisphosphate aldolase
Echinococcus multilocularis EmuJ_000993700   fructose bisphosphate aldolase class I
Echinococcus multilocularis EmuJ_000980100   fructose 1,6 bisphosphate aldolase
Echinococcus multilocularis EmuJ_000905600   fructose 1,6 bisphosphate aldolase
Homo sapiens ENSG00000109107   aldolase C, fructose-bisphosphate
Homo sapiens ENSG00000136872   aldolase B, fructose-bisphosphate
Homo sapiens ENSG00000149925   aldolase A, fructose-bisphosphate
Homo sapiens 102723572   uncharacterized protein LOC102723572
Leishmania braziliensis LbrM.35.1390   fructose-1,6-bisphosphate aldolase
Leishmania donovani LdBPK_361320.1   fructose-1,6-bisphosphate aldolase
Leishmania infantum LinJ.36.1320   fructose-1,6-bisphosphate aldolase
Leishmania major LmjF.36.1260   fructose-1,6-bisphosphate aldolase
Leishmania mexicana LmxM.36.1260   fructose-1,6-bisphosphate aldolase
Loa Loa (eye worm) LOAG_03230   fructose-bisphosphate aldolase 1
Loa Loa (eye worm) LOAG_04016   fructose-bisphosphate aldolase 2
Mus musculus ENSMUSG00000063129   aldolase 1 A, retrogene 2
Mus musculus ENSMUSG00000028307   aldolase B, fructose-bisphosphate
Mus musculus ENSMUSG00000030695   aldolase A, fructose-bisphosphate
Mus musculus ENSMUSG00000017390   aldolase C, fructose-bisphosphate
Mus musculus ENSMUSG00000059343   aldolase 1 A, retrogene 1
Neospora caninum NCLIV_050380   Fructose-bisphosphate aldolase (EC 4.1.2.13), related
Oryza sativa 4341496   Os06g0608700
Oryza sativa 4349897   Os11g0171300
Oryza sativa 4325027   Os01g0905800
Oryza sativa 4326074   Os01g0118000
Oryza sativa 4344545   Os08g0120600
Plasmodium berghei PBANKA_1308600   fructose-bisphosphate aldolase 2
Plasmodium falciparum PF3D7_1444800   fructose-bisphosphate aldolase
Plasmodium knowlesi PKNH_1237500   fructose-bisphosphate aldolase, putative
Plasmodium vivax PVX_118255   fructose 1,6-bisphosphate aldolase, putative
Plasmodium yoelii PY03709   Fructose-bisphosphate aldolase class-I
Schistosoma japonicum Sjp_0031630   ko:K01623 fructose-bisphosphate aldolase, class I [EC4.1.2.13A], putative
Schistosoma mansoni Smp_042160.1   fructose 16-bisphosphate aldolase
Schistosoma mansoni Smp_042160.2   fructose 16-bisphosphate aldolase
Schmidtea mediterranea mk4.015952.00   Fructose-bisphosphate aldolase
Schmidtea mediterranea mk4.030385.00  
Schmidtea mediterranea mk4.002266.03  
Schmidtea mediterranea mk4.014110.00  
Schmidtea mediterranea mk4.059262.00  
Trypanosoma brucei gambiense Tbg972.10.6830   fructose-bisphosphate aldolase, glycosomal putative
Trypanosoma brucei Tb11.v5.0531   fructose-bisphosphate aldolase, glycosomal, putative
Trypanosoma congolense TcIL3000_10_4760   fructose-bisphosphate aldolase, glycosomal, putative
Trypanosoma cruzi TcCLB.510301.10   fructose-bisphosphate aldolase, glycosomal, putative
Trypanosoma cruzi TcCLB.504163.40   fructose-bisphosphate aldolase, glycosomal, putative
Trypanosoma cruzi TcCLB.510301.20   fructose-bisphosphate aldolase, glycosomal, putative
Trypanosoma cruzi TcCLB.504163.50   fructose-bisphosphate aldolase, glycosomal, putative
Toxoplasma gondii TGME49_236040   fructose-1,6-bisphosphate aldolase
Toxoplasma gondii TGME49_321900   fructose-1,6-bisphosphate aldolase
Toxoplasma gondii TGME49_236050   fructose-bisphosphate aldolase, putative
Theileria parva TP01_0101   fructose-bisphosphate aldolase, putative

Essentiality

LmjF.36.1260 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.5620 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.5620 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.5620 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.5620 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F01F1.12 Caenorhabditis elegans embryonic lethal wormbase
CELE_F01F1.12 Caenorhabditis elegans slow growth wormbase
PBANKA_1308600 Plasmodium berghei Essential plasmo
TGME49_236040 Toxoplasma gondii Probably essential sidik
TGME49_236050 Toxoplasma gondii Probably essential sidik
TGME49_321900 Toxoplasma gondii Probably essential sidik
TGME49_236040 Toxoplasma gondii Essentiality uncertain sidik
TGME49_236050 Toxoplasma gondii Essentiality uncertain sidik
TGME49_321900 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Leishmania mexicana 130746   377156   377555  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with ALD inhibitors leads to reduced enzyme activity in vitro; . References: 10551371

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

  • Validation: inhibited in cell-free system
  • annotated by: aaronjr@u.washington.edu.
  • References: 10551371

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma brucei gambiense fructose-bisphosphate aldolase, glycosomal putative Compounds References
Oryctolagus cuniculus Fructose-bisphosphate aldolase A Compounds References
Plasmodium falciparum fructose-bisphosphate aldolase Compounds References
Trypanosoma brucei fructose-bisphosphate aldolase, glycosomal, putative Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0077 1 1
0.0292 0.9258 1
0.0566 0.2737 0.966
0.0269 0.6375 1
0.0179 0.2552 1
0.0099 0.2811 0.2814
0.0208 0.2932 1
0.0095 0.2725 0.2897
0.0543 0.2635 0.8723
0.009 0.2726 1
0.0109 0.2509 0.6503
0.0101 0.3106 1
0.0159 0.4544 1
0.0174 0.6123 1
0.0291 0.9258 1
0.0563 1 1
0.0095 0.2605 0.287
0.0587 0.4401 1
0.01 0.3297 1
0.0101 0.3106 1
0.0098 0.2953 0.3234
0.0323 0.9266 1
0.0088 0.5546 0.5
0.0159 0.4544 1
0.0097 0.4856 0.5
0.0174 0.6572 1
0.0083 0.418 0
0.0272 0.7808 0.9403
0.0089 0.3058 0.3234
0.0095 0.2724 0.2816
0.0093 0.2892 0.3039
0.0263 0.3465 0.372
0.083 1 1
0.0095 0.279 0.278
0.0095 0.2859 0.3138
0.0097 0.5082 1
0.0062 0.2769 1
0.0281 0.9258 1
0.0272 0.7808 0.9403
0.0298 0.9263 1
0.011 0.2504 1
0.009 1 0.5
0.0093 0.2509 1
0.011 1 1
0.011 0.2509 1
0.009 0.295 0.3234
0.0268 0.9266 1
0.0095 0.2812 0.2816
0.0095 0.2859 0.3138
0.0116 0.4714 1
0.0579 0.5455 1
0.011 0.2509 1
0.0120412 0.667736 0.903786
0.0088 0.3135 1
0.0317 0.3736 1
0.0601 0.3502 1
0.011 0.2779 1
0.0095 0.2826 0.3103
0.0317 0.3736 1
0.009 0.2726 1
0.0095 0.4401 1
0.0099 0.2775 0.9554
0.0089 0.253 1
0.0415307 1 1
0.0272 0.8815 0.8796
0.0272 0.8815 0.8796
0.0145 0.4635 1
0.0091 0.2766 0.2741
0.0323 0.9266 1
0.0601 0.3502 1
0.0618 0.3531 1
0.0554 0.3312 1
0.0566 0.3169 1
0.0088 0.8089 1
0.011 0.2828 0.9565
0.0197 0.2977 0.9592
0.0098 0.2779 1
0.0566 0.3169 1
0.0088 0.2565 0.3039
0.0275 0.3191 1
0.0323 0.9266 1
0.0288 0.926 1
0.0099 0.2714 0.954
0.0566 0.3169 1
0.0207 0.8885 1
0.0095 0.2859 0.3138
0.0176 0.2504 1
0.0084 0.2526 1
0.0553 1 1
0.0091 0.5 0.5
0.009 0.5 0.5
0.0284 0.3526 1
0.009 0.2726 1
0.0089 1 1
0.0294 0.5332 1
0.0201929 0.669447 1
0.0304 0.5918 1
0.0104357 0.404212 0.5055
0.011 0.2509 1
0.0317 0.3736 1
0.011 0.286 0.3903
0.0096 0.2573 1
0.0095 0.279 0.278
0.062 0.3196 1
0.011 1 1
0.0095 0.2948 0.3199
0.0089 0.3058 0.3234
0.0085 0.2621 0.4574
0.0273 0.9266 1
0.0096 0.2514 1
0.0273 0.9258 1
0.0275 0.3191 1
0.0106 0.5461 0.5
0.0092 0.9066 1
0.0288 0.926 1
0.0532 1 1
0.0097 0.4904 1
0.0263 0.3465 0.372
0.0422091 0.46257 1
0.01 0.7614 1
0.0099 0.2811 0.2814
0.0842 1 1
0.0293 0.9266 1
0.0095 0.2725 0.2897
0.0272 0.8815 0.8796
0.0579 0.5455 1
0.0618 0.3531 1
0.0268 0.3647 1
0.0091 0.279 0.9183
0.0579 0.5455 1
0.0106 0.535 0.5
0.0297 0.9238 1

Assayability

Assay information

  • Assay for Aldolase (4.1.2.13 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Leishmania mexicana ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    LmjF.36.1260 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Leishmania mexicana ( 1 )

Bibliographic References

8 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.36.1260 (Leishmania major), fructose-1,6-bisphosphate aldolase
Title for this comment
Comment