Detailed view for LmjF.10.0390

Basic information

TDR Targets ID: 21475
Leishmania major, folate/biopterin transporter, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.5094 | Length (AA): 732 | MW (Da): 79638 | Paralog Number: 9

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 12

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03092   BT1 family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
467 630 2cfq (A) 8 163 13.00 0.00000082 0.01 0.32 -0.82
543 617 3uxy (A) 117 190 31.00 0.74 0.04 0.113359 2.32

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_129265)

Species Accession Gene Product
Leishmania braziliensis LbrM.10.0380   folate/biopterin transporter, putative
Leishmania braziliensis LbrM.10.0370   folate/biopterin transporter, putative
Leishmania braziliensis LbrM.10.1460   pteridine transporter, putative
Leishmania braziliensis LbrM.10.0020   pteridin transporter, putative
Leishmania braziliensis LbrM.10.0410   folate/biopterin transporter, putative
Leishmania braziliensis LbrM.10.0400   folate/biopterin transporter, putative
Leishmania braziliensis LbrM.10.0390   folate/biopterin transporter, putative
Leishmania donovani LdBPK_100360.1   folate/biopterin transporter, putative
Leishmania donovani LdBPK_190870.1   folate/biopterin transporter, putative
Leishmania donovani LdBPK_100370.1   folate/biopterin transporter, putative
Leishmania donovani LdBPK_100420.1   folate/biopterin transporter, putative
Leishmania donovani LdBPK_060310.1   folate/biopterin transporter, putative
Leishmania infantum LinJ.19.0870   folate/biopterin transporter, putative
Leishmania infantum LinJ.06.0310   folate/biopterin transporter, putative
Leishmania infantum LinJ.10.0390   folate/biopterin transporter, putative
Leishmania infantum LinJ.10.0360   folate/biopterin transporter, putative
Leishmania infantum LinJ.10.0380   folate/biopterin transporter, putative
Leishmania infantum LinJ.10.0370   S-adenosylmethionine transporter
Leishmania infantum LinJ.10.1450   pteridine transporter, putative
Leishmania infantum LinJ.10.0420   folate/biopterin transporter, putative
Leishmania infantum LinJ.10.0400   folate/biopterin transporter, putative
Leishmania infantum LinJ.10.0410   folate/biopterin transporter, putative
Leishmania major LmjF.10.1310   pteridine transporter, putative
Leishmania major LmjF.10.0370   folate/biopterin transporter, putative
Leishmania major LmjF.06.0310   folate/biopterin transporter, putative
Leishmania major LmjF.10.0380   folate/biopterin transporter, putative
Leishmania major LmjF.10.0360   folate/biopterin transporter, putative
Leishmania major LmjF.10.0020   pteridin transporter, putative
Leishmania major LmjF.10.0385   folate/biopterin transporter, putative
Leishmania major LmjF.19.0920   folate/biopterin transporter, putative
Leishmania major LmjF.10.0400   folate/biopterin transporter, putative
Leishmania major LmjF.10.0390   folate/biopterin transporter, putative
Leishmania mexicana LmxM.10.1310   pteridine transporter, putative
Leishmania mexicana LmxM.10.0380  
Leishmania mexicana LmxM.06.0310   pteridine transporter, putative
Leishmania mexicana LmxM.04.0020   pteridine transporter (truncated), putative
Leishmania mexicana LmxM.10.0370   folate/biopterin transporter, putative
Leishmania mexicana LmxM.10.0400   pteridine transporter ft5, putative
Leishmania mexicana LmxM.10.0350   pteridine transporter ft5, putative
Leishmania mexicana LmxM.19.0920   pteridine transporter, putative
Leishmania mexicana LmxM.10.0360   pteridine transporter ft6, putative
Neospora caninum NCLIV_039560   BT1 transmembrane domain-containing protein, putative
Neospora caninum NCLIV_026780   folate/methotrexate transporter, putative
Trypanosoma brucei gambiense Tbg972.8.3370   folate transporter, putative,expression site-associated gene 10 (ESAG10) protein, putative
Trypanosoma brucei gambiense Tbg972.10.11110   pteridine transporter, putative
Trypanosoma brucei gambiense Tbg972.8.3340   folate transporter, putative,expression site-associated gene 10 (ESAG10) protein, putative
Trypanosoma brucei gambiense Tbg972.1.1710   pteridine transporter, putative
Trypanosoma brucei gambiense Tbg972.1.1690   pteridine transporter, putative
Trypanosoma brucei gambiense Tbg972.8.3350   folate transporter, putative,expression site- associated gene 10 (ESAG10) protein, putative
Trypanosoma brucei gambiense Tbg972.1.1680   pteridine transporter, putative
Trypanosoma brucei Tb927.8.3650   folate transporter, putative
Trypanosoma brucei Tb11.v5.0766   folate transporter, putative
Trypanosoma brucei Tb927.10.9080   pteridine transporter, putative
Trypanosoma brucei Tb927.1.2820   pteridine transporter, putative
Trypanosoma brucei Tb927.1.2880   pteridine transporter, putative
Trypanosoma brucei Tb927.1.2850   pteridine transporter, putative
Trypanosoma brucei Tb927.8.3620   folate transporter, putative
Trypanosoma brucei Tb927.8.3630   folate transporter, putative
Trypanosoma congolense TcIL3000_10_7850   pteridine transporter, putative
Trypanosoma congolense TcIL3000_0_13340   folate transporter, putative
Trypanosoma congolense TcIL3000_0_06950   folate transporter, putative
Trypanosoma cruzi TcCLB.511575.130   folate/pteridine transporter, putative
Trypanosoma cruzi TcCLB.508027.40   folate/pteridine transporter, putative
Trypanosoma cruzi TcCLB.506633.90   folate/pteridine transporter, putative
Toxoplasma gondii TGME49_260220   folate/biopterin transporter subfamily protein

Essentiality

LmjF.10.0390 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.2820 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.1.2820 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.2820 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.2820 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.3620 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.3620 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.3620 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.3620 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.9080 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.9080 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.9080 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.9080 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_260220 Toxoplasma gondii Probably non-essential sidik
TGME49_260220 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania donovani No drug identifiers listed for this gene.
Annotator: crowther@u.washington.edu. Comment: deletion of a folate transport gene led to lack of growth in media with pterins only (no folate). References: 2090944 2610143

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Leishmania major biopterin transporter, putative;with=GeneDB:LinJ35_V3.5120 631 aa 36.2% 625 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.10.0390 (Leishmania major), folate/biopterin transporter, putative
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