Detailed view for LmjF.25.0960

Basic information

TDR Targets ID: 23078
Leishmania major, gamma-tubulin

Source Database / ID: TriTrypDB / LmjF.25.0960  GeneDB / LmjF.25.0960

pI: 6.3855 | Length (AA): 447 | MW (Da): 50031 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: ND

Pfam domains

PF00091   Tubulin/FtsZ family, GTPase domain
PF03953   Tubulin C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0000930   gamma-tubulin complex  
GO:0031122   cytoplasmic microtubule organization and biogenesis  
GO:0007020   microtubule nucleation  
GO:0005874   microtubule  
GO:0043234   protein complex  
GO:0005525   GTP binding  
GO:0003924   GTPase activity  
GO:0051258   protein polymerization  
GO:0007017   microtubule-based process  

Network

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models: 4

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 440 1z5v (A) 2 440 67.00 0 1 1.82 -1.56
3 441 1sa0 (B) 2 438 34.00 0 1 1.47 -1.61
3 438 1sa0 (A) 2 435 33.00 0 1 1.5 -1.64
2 444 3cb2 (A) 2 444 67.00 0 1 1.88565 -1.64

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127652)
Species Accession Gene Product
Arabidopsis thaliana AT5G05620   tubulin gamma-2 chain
Arabidopsis thaliana AT3G61650   tubulin gamma-1 chain
Babesia bovis BBOV_IV007250   tubulin gamma chain
Brugia malayi Bm1_55245   Tubulin gamma chain
Candida albicans CaO19.8823   gamma tubulin-like protein
Candida albicans CaO19.1238   gamma tubulin-like protein
Caenorhabditis elegans CELE_F58A4.8   Protein TBG-1
Cryptosporidium hominis Chro.70228   gamma-tubulin
Cryptosporidium parvum cgd7_1980   gamma tubulin
Dictyostelium discoideum DDB_G0271738   gamma tubulin
Drosophila melanogaster Dmel_CG17566   gamma-Tubulin at 37C
Drosophila melanogaster Dmel_CG3157   gamma-Tubulin at 23C
Echinococcus granulosus EgrG_001099700   tubulin gamma 1 chain
Entamoeba histolytica EHI_008240   tubulin gamma chain
Echinococcus multilocularis EmuJ_001099700   tubulin gamma 1 chain
Giardia lamblia GL50803_114218   Gamma tubulin
Homo sapiens ENSG00000131462   tubulin, gamma 1
Homo sapiens ENSG00000037042   tubulin, gamma 2
Leishmania braziliensis LbrM.25.0840   gamma-tubulin
Leishmania donovani LdBPK_250990.1   tubulin gamma chain
Leishmania infantum LinJ.25.0990   gamma-tubulin
Leishmania major LmjF.25.0960 this record   gamma-tubulin
Leishmania mexicana LmxM.25.0960   gamma-tubulin
Loa Loa (eye worm) LOAG_02400   tubulin gamma chain
Mus musculus ENSMUSG00000045007   tubulin, gamma 2
Mus musculus ENSMUSG00000035198   tubulin, gamma 1
Neospora caninum NCLIV_046130   Beta-tubulin, related
Oryza sativa 4337861   Os05g0156600
Plasmodium berghei PBANKA_1226600   tubulin gamma chain, putative
Plasmodium falciparum PF3D7_0803700   tubulin gamma chain
Plasmodium knowlesi PKNH_0115200   tubulin gamma chain, putative
Plasmodium vivax PVX_093560   tubulin gamma chain, putative
Plasmodium yoelii PY00808   tubulin gamma chain
Saccharomyces cerevisiae YLR212C   Tub4p
Schistosoma japonicum Sjp_0036720   ko:K10389 tubulin gamma, putative
Schistosoma mansoni Smp_034670   tubulin subunit gamma
Schmidtea mediterranea mk4.005127.01   Tubulin gamma chain
Schmidtea mediterranea mk4.005127.02   Tubulin gamma chain
Schmidtea mediterranea mk4.005127.00   Tubulin gamma chain
Schmidtea mediterranea mk4.006572.00   Tubulin gamma chain
Trypanosoma brucei gambiense Tbg972.3.550   gamma tubulin
Trypanosoma brucei Tb927.3.910   tubulin gamma chain
Trypanosoma congolense TcIL3000_0_03350   tubulin gamma chain
Trypanosoma cruzi TcCLB.511867.190   tubulin gamma chain
Theileria parva TP03_0233   tubulin gamma chain, putative
Trichomonas vaginalis TVAG_109820   tubulin gamma chain, putative
Trichomonas vaginalis TVAG_369500   tubulin, putative

Essentiality

LmjF.25.0960 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.910 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.910 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.910 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.910 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F58A4.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_F58A4.8 Caenorhabditis elegans sterile wormbase
YLR212C Saccharomyces cerevisiae inviable yeastgenome

Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania amazonensis No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 09/08/82. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; . References: 2392684
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania amazonensis No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 09/08/82. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; . References: 2392684
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania mexicana 79195  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 2044859
morphology (PATO:0000051) abnormal (PATO:0000460) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from visible phenotype (ECO:0000176) Leishmania mexicana 79195  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied. References: 2044859
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania mexicana 79195  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied. References: 2044859
cell differentiation (GO:0030154) disrupted (PATO:0001507) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania mexicana 79195  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with antimicrotubule agents leads to disrupted differentiation from promastigote to amastigote of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied. References: 2044859
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania amazonensis 586034  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 95480-32-3. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied. References: 8468004
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania amazonensis 13316   39854   79195   544264   551075   552236   554547   555991   557331   559236   559237   559604   561797   562909   564379   564831   568817   568820   568821   569525  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 29091-21-2. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied. References: 7818612
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania major 39854   250338   536202  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 121-50-6; Drug: 394-25-2; Drug: 14371-82-5; Drug: 22227-59-4; Drug: 174824-16-9; Drug: 176177-41-6. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 8849257
microtubule polymerization (GO:0046785) decreased (PATO:0000468) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from specific protein inhibition (ECO:0000020) Leishmania amazonensis 2976   8265   27599   67873   86876   250338   327961   371083   376038   543486   543487   559148   560712   572272   575226   588233  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 16037-91-5. chemical inhibition with antimicrotubule agents prevents mictotubule formation in vitro with purified tubulin and in live cells; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 10029309
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania amazonensis 2976   8265   27599   67873   86876   250338   327961   371083   376038   543486   543487   559148   560712   572272   575226   588233  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 16037-91-5. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 10029309
microtubule polymerization (GO:0046785) increased (PATO:0000470) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani 2976   8265   559148  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with microtubule stabilising agent forces mictotubule formation in live cells and interrupts cell cycle; unclear as to which topoisomerase II gene/enzyme was studied. References: 10427726
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani 2976   8265   559148  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with microtubule stabilising agent forces mictotubule formation in live cells and interrupts cell cycle; unclear as to which topoisomerase II gene/enzyme was studied. References: 10427726
growth (GO:0040007) drug insensitive (PATO:0000528) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani 2976   8265   559148  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with microtubule stabilising agent generated cell line resistant to drug with a MDR phenotype; reversible with Ca2+-channel blocker verapamil; unclear as to which topoisomerase II gene/enzyme was studied. References: 10427727
growth (GO:0040007) drug insensitive (PATO:0000528) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania infantum 37094   39854   79195   250338   551075   562909  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 10546848
microtubule polymerization (GO:0046785) decreased (PATO:0000468) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from specific protein inhibition (ECO:0000020) Leishmania donovani 2976   8265   80107   559148   576689   576690  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 313705-89-4; Drug: 157207-90-4. chemical inhibition with antimicrotubule agents leads to abnormal cell morphology, cell division, mitosis, organelle localization & organization, and slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied. References: 11071278
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani 2976   8265   80107   559148   576689   576690  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 313705-89-4; Drug: 157207-90-4. chemical inhibition with antimicrotubule agents leads to abnormal cell morphology, cell division, mitosis, organelle localization & organization, and slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied. References: 11071278
cytokinesis (GO:0000910) discontinuous (PATO:0000690) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from visible phenotype (ECO:0000176) Leishmania donovani 2976   8265   80107   559148   576689   576690  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 313705-89-4; Drug: 157207-90-4. chemical inhibition with antimicrotubule agents leads to abnormal cell morphology, cell division, mitosis, organelle localization & organization, and slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 11071278
mitotic sister chromatid segregation (GO:0000070) discontinuous (PATO:0000690) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from visible phenotype (ECO:0000176) Leishmania donovani 2976   8265   80107   559148   576689   576690  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 313705-89-4; Drug: 157207-90-4. chemical inhibition with antimicrotubule agents leads to abnormal cell morphology, cell division, mitosis, organelle localization & organization, and slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 11071278
organelle localization (GO:0051640) discontinuous (PATO:0000690) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from visible phenotype (ECO:0000176) Leishmania donovani 2976   8265   80107   559148   576689   576690  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 313705-89-4; Drug: 157207-90-4. chemical inhibition with antimicrotubule agents leads to abnormal cell morphology, cell division, mitosis, organelle localization & organization, and slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied. References: 11071278
morphology (PATO:0000051) abnormal (PATO:0000460) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from visible phenotype (ECO:0000176) Leishmania donovani 2976   8265   80107   559148   576689   576690  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 313705-89-4; Drug: 157207-90-4. chemical inhibition with antimicrotubule agents leads to abnormal cell morphology, cell division, mitosis, organelle localization & organization, and slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied. References: 11071278
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani 12596   13316   15907   26907   78691   79195   527824   528258   531885   531886   532164   532165   532166   532195   532196   532197   532198   532199   532642   532643   532644   536969   536970   544264   552236   554547   559604   561797   564379   568817   568820   568821  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 10228-56-5; Drug: 36317-95-0; Drug: 62409-47-6; Drug: 62745-74-8; Drug: 204634-79-7; Drug: 483279-40-9; Drug: 483279-41-0; Drug: 483279-42-1; Drug: 483279-43-2; Drug: 483279-44-3; Drug: 483279-45-4; Drug: 483279-46-5; Drug: 483279-47-6; Drug: 88-91-5; Drug: 107-10-8; Drug: 1930-72-9; Drug: 97-08-5; Drug: 97-09-6; Drug: 62443-96-3; Drug: 483279-33-0; Drug: 483279-34-1; Drug: 483279-35-2; Drug: 483279-36-3; Drug: 483279-37-4; Drug: 483279-38-5; Drug: 483279-39-6. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied. References: 12161141
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania donovani 12596   13316   15907   26907   78691   79195   527824   528258   531885   531886   532164   532165   532166   532195   532196   532197   532198   532199   532642   532643   532644   536969   536970   544264   552236   554547   559604   561797   564379   568817   568820   568821  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 10228-56-5; Drug: 36317-95-0; Drug: 62409-47-6; Drug: 62745-74-8; Drug: 204634-79-7; Drug: 483279-40-9; Drug: 483279-41-0; Drug: 483279-42-1; Drug: 483279-43-2; Drug: 483279-44-3; Drug: 483279-45-4; Drug: 483279-46-5; Drug: 483279-47-6; Drug: 88-91-5; Drug: 107-10-8; Drug: 1930-72-9; Drug: 97-08-5; Drug: 97-09-6; Drug: 62443-96-3; Drug: 483279-33-0; Drug: 483279-34-1; Drug: 483279-35-2; Drug: 483279-36-3; Drug: 483279-37-4; Drug: 483279-38-5; Drug: 483279-39-6. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 12161141
microtubule polymerization (GO:0046785) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Leishmania donovani 12596   13316   15907   26907   78691   79195   527824   528258   531885   531886   532164   532165   532166   532195   532196   532197   532198   532199   532642   532643   532644   536969   536970   544264   552236   554547   559604   561797   564379   568817   568820   568821  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 10228-56-5; Drug: 36317-95-0; Drug: 62409-47-6; Drug: 62745-74-8; Drug: 204634-79-7; Drug: 483279-40-9; Drug: 483279-41-0; Drug: 483279-42-1; Drug: 483279-43-2; Drug: 483279-44-3; Drug: 483279-45-4; Drug: 483279-46-5; Drug: 483279-47-6; Drug: 88-91-5; Drug: 107-10-8; Drug: 1930-72-9; Drug: 97-08-5; Drug: 97-09-6; Drug: 62443-96-3; Drug: 483279-33-0; Drug: 483279-34-1; Drug: 483279-35-2; Drug: 483279-36-3; Drug: 483279-37-4; Drug: 483279-38-5; Drug: 483279-39-6. chemical inhibition with antimicrotubule agents prevents mictotubule formation in vitro with purified tubulin; unclear as to which topoisomerase II gene/enzyme was studied. References: 12161141
cell cycle arrest (GO:0007050) abnormal (PATO:0000460) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani 79195  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 483279-40-9; Drug: 483279-44-3. chemical inhibition with antimicrotubule agents leads to cell cycle arrest in cell assay; unclear as to which topoisomerase II gene/enzyme was studied. References: 14645662
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania donovani 79195  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 19044-89-4; Drug: 19044-94-1; Drug: 483279-44-3; Drug: 483279-45-4; Drug: 619267-13-9; Drug: 619267-14-0; Drug: 619267-15-1; Drug: 619267-16-2; Drug: 619267-17-3; Drug: 619267-18-4; Drug: 619267-19-5; Drug: 619267-20-8; Drug: 619267-21-9; Drug: 619267-22-0; Drug: 619267-23-1; Drug: 619267-24-2; Drug: 619267-25-3; Drug: 619267-26-4; Drug: 678187-02-5; Drug: 678187-03-6; Drug: 678187-04-7; Drug: 678187-05-8; Drug: 678187-06-9; Drug: 678187-07-0; Drug: 678187-08-1; Drug: 678187-09-2; Drug: 678187-10-5; Drug: 678187-11-6; Drug: 678187-12-7; Drug: 678187-13-8; Drug: 678187-14-9. chemical inhibition with antimicrotubule agents leads to cell cycle arrest in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 15027874
microtubule polymerization (GO:0046785) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Leishmania donovani 79195  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 19044-89-4; Drug: 19044-94-1; Drug: 483279-44-3; Drug: 483279-45-4; Drug: 619267-13-9; Drug: 619267-14-0; Drug: 619267-15-1; Drug: 619267-16-2; Drug: 619267-17-3; Drug: 619267-18-4; Drug: 619267-19-5; Drug: 619267-20-8; Drug: 619267-21-9; Drug: 619267-22-0; Drug: 619267-23-1; Drug: 619267-24-2; Drug: 619267-25-3; Drug: 619267-26-4; Drug: 678187-02-5; Drug: 678187-03-6; Drug: 678187-04-7; Drug: 678187-05-8; Drug: 678187-06-9; Drug: 678187-07-0; Drug: 678187-08-1; Drug: 678187-09-2; Drug: 678187-10-5; Drug: 678187-11-6; Drug: 678187-12-7; Drug: 678187-13-8; Drug: 678187-14-9. chemical inhibition with antimicrotubule agents prevents mictotubule formation in vitro with purified tubulin; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 15027874
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 902779-03-7; Drug: 908843-57-2; Drug: 908843-58-3; Drug: 908843-61-8; Drug: 908843-63-0; Drug: 908843-68-5; Drug: 908843-70-9; Drug: 908843-73-2; Drug: 908843-74-3; Drug: 908843-75-4; Drug: 908843-76-5; Drug: 908843-77-6; Drug: 908843-78-7; Drug: 908843-79-8; Drug: 908843-80-1; Drug: 908843-81-2; Drug: 908843-82-3; Drug: 908843-84-5. chemical inhibition with antimicrotubule agents leads to cell cycle arrest in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 16675220
microtubule polymerization (GO:0046785) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 902779-03-7; Drug: 908843-57-2; Drug: 908843-58-3; Drug: 908843-61-8; Drug: 908843-63-0; Drug: 908843-68-5; Drug: 908843-70-9; Drug: 908843-73-2; Drug: 908843-74-3; Drug: 908843-75-4; Drug: 908843-76-5; Drug: 908843-77-6; Drug: 908843-78-7; Drug: 908843-79-8; Drug: 908843-80-1; Drug: 908843-81-2; Drug: 908843-82-3; Drug: 908843-84-5. chemical inhibition with antimicrotubule agents prevents mictotubule formation in vitro with purified tubulin; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 16675220
microtubule polymerization (GO:0046785) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Leishmania tarentolae 79195  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 483279-44-3; Drug: 619267-15-1. chemical inhibition with antimicrotubule agents prevents mictotubule formation in vitro with purified tubulin; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 16753146
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania mexicana 449723  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 209345-04-0; Drug: 58915-19-8; Drug: 209345-05-1; Drug: 218935-77-4; Drug: 268749-50-4. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 16918078
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania infantum 449723  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 209345-04-0; Drug: 58915-19-8; Drug: 209345-05-1; Drug: 218935-77-4; Drug: 268749-50-4. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 16918078
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania mexicana 449723  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 209345-04-0; Drug: 58915-19-8; Drug: 209345-05-1; Drug: 218935-77-4; Drug: 268749-50-4. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied. References: 16918078

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
 
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Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.9

Curated from literature

64 chemical compounds are associated with this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma brucei tubulin gamma chain Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Sus scrofa Tubulin 445 aa 34.0% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Bos taurus Tubulin beta chain 445 aa 34.0% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Homo sapiens tubulin, beta 4B class IVb 445 aa 34.5% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Trypanosoma brucei beta tubulin 442 aa 34.8% 443 aa Compounds References
Bos taurus Tubulin 445 aa 34.0% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Homo sapiens tubulin, beta 2B class IIb 445 aa 34.0% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Homo sapiens tubulin, beta 6 class V 446 aa 34.0% 438 aa Compounds References
Plasmodium falciparum tubulin beta chain 445 aa 35.9% 443 aa Compounds References
Bos taurus Tubulin 85 aa 39.5% 81 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References
Leishmania major beta tubulin 443 aa 35.2% 438 aa Compounds References

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.25.0960 (Leishmania major), gamma-tubulin
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