Detailed view for PF3D7_1401500

Basic information

TDR Targets ID: 2350
Plasmodium falciparum, esterase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.8968 | Length (AA): 373 | MW (Da): 43737 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12146   Serine aminopeptidase, S33

Gene Ontology

Mouse over links to read term descriptions.
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0016787   hydrolase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
20 360 1u2e (A) 5 283 13.00 0 0.51 0.72 -0.4
25 360 1zoi (A) 3 276 16.00 0 0.93 0.78 -0.08
170 223 1r3d (A) 82 131 32.00 0.71 0.18 0.286472 1.07
298 361 1tqh (A) 182 245 14.00 0.00097 0.05 0.259882 0.4

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 16 hs, early trophozoite, late ring. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 24 hs, merozoite, sporozoite, early ring, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 48 hs, gametocyte, late trophozoite, Sporozoite, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 40 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Oocyst, Female Gametocyte. Zanghi G Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_144587)

Species Accession Gene Product
Entamoeba histolytica EHI_086370   hydrolase, alpha/beta fold family domain containing protein
Neospora caninum NCLIV_001135   hypothetical protein
Plasmodium falciparum PF3D7_1401500   esterase, putative
Plasmodium falciparum PF3D7_0936700   lysophospholipase, putative
Plasmodium falciparum PF3D7_1252600   esterase, putative
Plasmodium vivax PVX_097560   lysophospholipase, putative
Plasmodium vivax PVX_203290   unspecified product
Toxoplasma gondii TGME49_305260   hypothetical protein
Theileria parva TP03_0739   hypothetical protein
Theileria parva TP03_0200   hypothetical protein
Theileria parva TP04_0172   hypothetical protein
Theileria parva TP03_0738   hypothetical protein
Trichomonas vaginalis TVAG_364450   conserved hypothetical protein

Essentiality

PF3D7_1401500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_305260 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) multi-cellular organism (CARO:0000012) bloodstream stage (PLO:0040) in vivo inhibition (TDR:00016) Plasmodium falciparum 2432   14451   35556   301577   414933   415749   539326   539352   539353   539354   539355   542835   542836   558535   568684   568685   573809   583295   585401  
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 8022653

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Plasmodium falciparum lysophospholipase, putative 371 aa 42.0% 362 aa Compounds References
Rattus norvegicus Monoglyceride lipase 303 aa 21.5% 265 aa Compounds References
Plasmodium falciparum lysophospholipase, putative 353 aa 46.2% 340 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0144 0.3377 0.92
0.0644 0.3094 0.8044
0.076 0.3329 0.332
0.0293 1 1
0.0477 0.4902 0.7373
0.0355 0.4784 1
0.0144 0.2804 1
0.0293 0.3562 0.8009
0.0509 0.7285 1
0.0644 0.309 0.8048
0.0308 0.3458 0.6786
0.0743 0.7271 0.8055
0.0292 0.3473 0.6768
0.0533 0.7313 1
0.0305 0.346 0.6687
0.0308 0.3448 0.6786
0.0483 0.4806 1
0.0145 1 1
0.0297 0.3466 0.3556
0.0146 0.2648 0.2978
0.0509 0.7285 1
0.0293 1 1
0.0145 0.2804 1
0.0143 0.4229 1
0.0326 0.3443 0.6114
0.0483 0.4816 1
0.0178 1 1
0.031 0.3555 0.681
0.051 0.7305 1
0.0308 0.3459 0.6786
0.0147 0.2842 0.3031
0.051 0.7305 1
0.0146 0.2867 1
0.0301 0.331 0.6717
0.0142 0.9044 0.8578
0.0297 0.3466 0.3556
0.0177 0.9044 0.8578
0.0478 0.5187 0.7469
0.0308 0.3469 0.6767
0.0173 1 1
0.0299 0.2979 0.6699
0.0144 0.2648 0.2978
0.03 0.3474 0.6792
0.0334 0.3462 0.6815
0.0308 0.3453 0.6712
0.0644 0.309 0.8048
0.0144 0.2782 1
0.0296 1 1
0.0145 1 1
0.0188149 1 1
0.0327 0.336 0.6878
0.0323 0.3345 0.5898
0.0861 0.727 0.8055
0.0146 0.2906 0.2921
0.031 0.3555 0.681
0.0509 0.7305 1
0.0355 0.533 1
0.0644 0.309 0.8048
0.0297 0.3466 0.3556
0.0146 0.3287 0.92
0.0145 0.2779 1
0.0069 0.4643 0.9034
0.0533 0.7305 1
0.0457 0.4802 1
0.0145 0.2804 1
0.0449 0.4729 0.747
0.0793 0.6586 0.7901
0.0297 0.3466 0.3556
0.0326 0.3443 0.6114
0.0326 0.3443 0.6114
0.0589 0.4367 0.4674
0.0308 0.3459 0.6786
0.0312 0.3458 0.6786
0.0299 0.2979 0.6699
0.0326 0.3443 0.6114
0.0449 0.4729 0.747
0.0145 0.2779 1
0.0178 0.9186 0.9186
0.0178 0.9044 0.8578
0.0301 0.3455 0.6687
0.0644 0.309 0.8048
0.0678 0.6077 0.6697
0.0178 0.3139 1
0.0334 0.3387 0.5973
0.0291 0.3557 0.6811

Assayability

Assay information

  • Assay for Phospholipase B (3.1.1.5 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

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Gene identifier PF3D7_1401500 (Plasmodium falciparum), esterase, putative
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