Detailed view for TGME49_268560

Basic information

TDR Targets ID: 259446
Toxoplasma gondii, XPG N-terminal domain-containing protein
Source Database / ID:  ToxoDB 

pI: 4.969 | Length (AA): 2004 | MW (Da): 217599 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00752   XPG N-terminal domain
PF00867   XPG I-region

Gene Ontology

Mouse over links to read term descriptions.
GO:0016788   hydrolase activity, acting on ester bonds  
GO:0005634   nucleus  
GO:0004519   endonuclease activity  
GO:0004518   nuclease activity  
GO:0003824   catalytic activity  
GO:0003697   single-stranded DNA binding  
GO:0003677   DNA binding  
GO:0006289   nucleotide-excision repair  
GO:0006281   DNA repair  

Metabolic Pathways

Nucleotide excision repair (KEGG)

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 247 3qe9 (Y) 2 240 20.00 0.000000000006 0.77 0.296355 0.48
7 111 2izo (A) 1 159 31.00 0.000025 0.97 0.308995 0.85
1090 1426 4hm9 (A) 75 425 10.00 0 0.02 0.200164 -0.07
1109 1337 5cwm (A) 8 220 15.00 0 0.1 0.239271 -0.75
1339 1647 3ory (A) 53 333 26.00 0 1 0.162192 1.17
1409 1642 4q0w (B) 88 984 36.00 0 1 0.467766 0.29
1428 1550 5t9j (A) 116 241 39.00 0 0.97 0.348477 1.64
1753 2001 4x0j (A) 42 283 13.00 0 0.08 0.253251 -0.55

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128675)

Species Accession Gene Product
Arabidopsis thaliana AT3G28030   DNA repair protein UVH3
Babesia bovis BBOV_II000500   Rad2 endonuclease, putative
Brugia malayi Bm1_05790   XPG N-terminal domain containing protein
Candida albicans CaO19.1324   similar to RAD13, excision repair protein
Candida albicans CaO19.8904   similar to RAD13, excision repair protein
Caenorhabditis elegans CELE_F57B10.6   Protein XPG-1
Cryptosporidium hominis Chro.40058   RAD2
Cryptosporidium parvum cgd4_440   XPG, DNA excision repair protein, flap endonuclease
Dictyostelium discoideum DDB_G0294290   xeroderma pigmentosum group G family protein
Drosophila melanogaster Dmel_CG10890   mutagen-sensitive 201
Echinococcus granulosus EgrG_000321400   DNA repair protein complementing XP G cells
Entamoeba histolytica EHI_100400   DNA-repair protein, putative
Echinococcus multilocularis EmuJ_000321400   DNA repair protein complementing XP G cells
Homo sapiens ENSG00000270181   BIVM-ERCC5 readthrough
Homo sapiens ENSG00000134899   excision repair cross-complementation group 5
Loa Loa (eye worm) LOAG_00986   hypothetical protein
Mus musculus ENSMUSG00000026048   excision repair cross-complementing rodent repair deficiency, complementation group 5
Neospora caninum NCLIV_037790   RAD2 endonuclease, putative
Oryza sativa 4331991   Os03g0205400
Plasmodium berghei PBANKA_0303700   DNA repair protein RAD2, putative
Plasmodium falciparum PF3D7_0206000   DNA repair protein RAD2, putative
Plasmodium knowlesi PKNH_0414900   DNA repair protein RAD2, putative
Plasmodium vivax PVX_003735   DNA repair protein RAD2, putative
Plasmodium yoelii PY01122   XPG I-region, putative
Saccharomyces cerevisiae YGR258C   Rad2p
Schistosoma japonicum Sjp_0023590   DNA-repair protein rad13, putative
Schistosoma mansoni Smp_123940   xp-G/rad2 DNA repair endonuclease family
Schmidtea mediterranea mk4.006295.00  
Schmidtea mediterranea mk4.005914.00  
Toxoplasma gondii TGME49_268560   XPG N-terminal domain-containing protein
Theileria parva TP04_0530   DNA repair protein rad2, putative
Theileria parva TP04_0531   hypothetical protein, conserved
Trichomonas vaginalis TVAG_046610   flap endonuclease-1, putative

Essentiality

TGME49_268560 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_F57B10.6 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_0303700 Plasmodium berghei Dispensable plasmo
TGME49_268560 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens excision repair cross-complementation group 5 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0044 1 1
0.004 1 1
0.0044 0.8175 1
0.0044 1 1
0.0044 1 1
0.0073 0.2579 0.239
0.0074 1 1
0.015 1 1
0.0044 1 1
0.0036 0.3124 1
0.0044 1 1
0.0044 1 1
0.0173 1 1
0.0044 0.5851 1
0.0072 0.2769 0.3636
0.0044 1 1
0.0163 0.2876 0.8361
0.0036 1 1
0.0044 0.4298 1
0.0044 1 1
0.0044 1 1
0.0077 0.405 0.8087
0.0074 0.2554 0.2554
0.0078 0.6848 0.8055
0.0044 1 1
0.0077 0.8148 0.8148
0.0044 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_268560 (Toxoplasma gondii), XPG N-terminal domain-containing protein
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