Detailed view for TGME49_315620

Basic information

TDR Targets ID: 260865
Toxoplasma gondii, vacuolar ATP synthase subunit C, putative
Source Database / ID:  ToxoDB 

pI: 6.1921 | Length (AA): 404 | MW (Da): 46075 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03223   V-ATPase subunit C

Gene Ontology

Mouse over links to read term descriptions.
GO:0033180   proton-transporting V-type ATPase, V1 domain  
GO:0015078   hydrogen ion transmembrane transporter activity  
GO:0015991   ATP hydrolysis coupled proton transport  
GO:0015986   ATP synthesis coupled proton transport  

Metabolic Pathways

Oxidative phosphorylation (KEGG)
Global map (KEGG)
Phagosome (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
17 396 1u7l (A) 23 392 30.00 0 1 1.26299 -0.32

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_127946)

Species Accession Gene Product
Arabidopsis thaliana AT1G12840   V-type proton ATPase subunit C
Babesia bovis BBOV_III011050   vacuolar ATPase subunit C family protein
Brugia malayi Bm1_39100   V-ATPase subunit C family protein
Candida albicans CaO19.2166   vacuolar ATPase (subunit C)
Candida albicans CaO19.9712   vacuolar ATPase (subunit C)
Caenorhabditis elegans CELE_Y38F2AL.3   Protein VHA-11, isoform A
Cryptosporidium hominis Chro.40070   vacuolar ATP synthase
Cryptosporidium parvum cgd4_540   putative vacuolar ATP synthase subunit C
Dictyostelium discoideum DDB_G0284473   H(+)-transporting ATPase
Drosophila melanogaster Dmel_CG8048   Vacuolar H[+] ATPase 44kD subunit
Entamoeba histolytica EHI_092600   vacuolar ATP synthase subunit C, putative
Giardia lamblia GL50803_87058   Vacuolar ATP synthase subunit C
Homo sapiens ENSG00000155097   ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
Leishmania braziliensis LbrM.18.0620   vacuolar ATP synthase subunit c, putative
Leishmania donovani LdBPK_180560.1   vacuolar ATP synthase subunit c, putative
Leishmania infantum LinJ.18.0560   vacuolar ATP synthase subunit c, putative
Leishmania major LmjF.18.0560   vacuolar ATP synthase subunit c, putative
Leishmania mexicana LmxM.18.0560   vacuolar ATP synthase subunit c, putative
Loa Loa (eye worm) LOAG_03931   V-ATPase subunit C family protein
Mus musculus ENSMUSG00000022295   ATPase, H+ transporting, lysosomal V1 subunit C1
Neospora caninum NCLIV_058310   vacuolar ATP synthase subunit c, putative
Oryza sativa 4339800   Os05g0593100
Plasmodium berghei PBANKA_0207200   V-type proton ATPase subunit C, putative
Plasmodium falciparum PF3D7_0106100   V-type proton ATPase subunit C, putative
Plasmodium knowlesi PKNH_0207400   V-type proton ATPase subunit C, putative
Plasmodium vivax PVX_081465   vacuolar ATP synthase subunit c, putative
Plasmodium yoelii PY05774   vacuolar ATP synthase subunit c
Saccharomyces cerevisiae YKL080W   H(+)-transporting V1 sector ATPase subunit C
Schmidtea mediterranea mk4.000790.02   V-type proton ATPase subunit C
Trypanosoma brucei gambiense Tbg972.10.17240   vacuolar ATP synthase subunit c, putative
Trypanosoma brucei Tb927.10.14040   vacuolar ATP synthase subunit c, putative
Trypanosoma congolense TcIL3000_10_11650   vacuolar ATP synthase subunit c, putative
Trypanosoma cruzi TcCLB.511277.240   vacuolar ATP synthase subunit c, putative
Toxoplasma gondii TGME49_315620   vacuolar ATP synthase subunit C, putative
Theileria parva TP02_0034   vacuolar ATP synthase subunit C, putative
Trichomonas vaginalis TVAG_037610   vacuolar ATP synthase subunit C, putative

Essentiality

TGME49_315620 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.14040 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.14040 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.14040 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.14040 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y38F2AL.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y38F2AL.3 Caenorhabditis elegans larval arrest wormbase
CELE_Y38F2AL.3 Caenorhabditis elegans larval lethal wormbase
CELE_Y38F2AL.3 Caenorhabditis elegans slow growth wormbase
CELE_Y38F2AL.3 Caenorhabditis elegans sterile wormbase
PBANKA_0207200 Plasmodium berghei Essential plasmo
TGME49_315620 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Manduca sexta V-type proton ATPase subunit C Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0036 1 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

4 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_315620 (Toxoplasma gondii), vacuolar ATP synthase subunit C, putative
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