Detailed view for TGME49_267360

Basic information

TDR Targets ID: 261849
Toxoplasma gondii, histone deacetylase SIR2-like
Source Database / ID:  ToxoDB 

pI: 6.8291 | Length (AA): 1715 | MW (Da): 187186 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02146   Sir2 family

Gene Ontology

Mouse over links to read term descriptions.
GO:0070403   NAD binding  

GO:0008270   zinc ion binding  
GO:0045449   regulation of transcription  
GO:0006476   protein amino acid deacetylation  
GO:0006342   chromatin silencing  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
189 522 5mf6 (A) 47 274 44.00 0 1 0.102652 1.1
270 523 5mf6 (A) 10 275 29.00 0 0.84 0.373305 0.5
362 396 4utv (A) 133 165 55.00 0.043 0.03 0.420308 0.81
1247 1387 4mh6 (A) 9 148 11.00 0.51 0.01 0.243916 -0.88
1270 1567 5a7y (A) 10 290 14.00 0 0.75 0.148961 0.25
1495 1581 5i6g (A) 2176 2310 8.00 0 0 0.352929 -2.77
1518 1573 5hmo (A) 816 874 45.00 0.00014 0.46 0.930553 -5.04
1537 1572 5j73 (A) 7 42 44.00 0.5 0.31 0.993891 -5.14

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_130121)

Species Accession Gene Product
Arabidopsis thaliana AT5G55760   sirtuin 1
Babesia bovis BBOV_I003070   transcriptional regulator, Sir2 family domain containing protein
Brugia malayi Bm1_16975   transcriptional regulator, Sir2 family protein
Caenorhabditis elegans CELE_C06A5.11   Protein SIR-2.4
Drosophila melanogaster Dmel_CG6284   CG6284 gene product from transcript CG6284-RB
Echinococcus granulosus EgrG_000908100   NAD dependent deacetylase sirtuin 6
Echinococcus granulosus EgrG_000908200   alpha 12 mannosyltransferase Smp3
Echinococcus multilocularis EmuJ_000908100   NAD dependent deacetylase sirtuin 6
Echinococcus multilocularis EmuJ_000908200   alpha 1,2 mannosyltransferase Smp3
Homo sapiens ENSG00000077463   sirtuin 6
Loa Loa (eye worm) LOAG_07562   transcriptional regulator
Mus musculus ENSMUSG00000034748   sirtuin 6
Neospora caninum NCLIV_038820   Sir2 domain-containing protein, putative
Oryza sativa 4335342   Os04g0271000
Plasmodium berghei PBANKA_1315100   transcriptional regulatory protein sir2b
Plasmodium falciparum PF3D7_1451400   transcriptional regulatory protein sir2b
Plasmodium knowlesi PKNH_1230700   transcriptional regulatory protein sir2b, putative
Plasmodium vivax PVX_117910   hypothetical protein, conserved
Plasmodium yoelii PY01626   sir2-like protein
Schistosoma japonicum Sjp_0080320   expressed protein
Schistosoma japonicum Sjp_0307950   expressed protein
Schistosoma japonicum Sjp_0129710   expressed protein
Schistosoma japonicum Sjp_0307780   expressed protein
Schistosoma japonicum Sjp_0219540   expressed protein
Schistosoma japonicum Sjp_0028930   ko:K00775 NAD(P)-arginine ADP-ribosyltransferase [EC2.4.2.31], putative
Schistosoma mansoni Smp_134630   chromatin regulatory protein sir2
Schmidtea mediterranea mk4.000591.01   NAD-dependent protein deacetylase sirtuin-6
Toxoplasma gondii TGME49_267360   histone deacetylase SIR2-like
Theileria parva TP01_0527   hypothetical protein, conserved

Essentiality

TGME49_267360 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1315100 Plasmodium berghei Dispensable plasmo
TGME49_267360 this record Toxoplasma gondii Essentiality uncertain sidik
TGME49_267360 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0309 0.2609 0.5985

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

5 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_267360 (Toxoplasma gondii), histone deacetylase SIR2-like
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