Detailed view for TGME49_230450

Basic information

TDR Targets ID: 262835
Toxoplasma gondii, bifunctional GMP synthase/glutamine amidotransferase protein
Source Database / ID:  ToxoDB 

pI: 6.6629 | Length (AA): 569 | MW (Da): 62295 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00117   Glutamine amidotransferase class-I
PF00958   GMP synthase C terminal domain
PF02540   NAD synthase

Gene Ontology

Mouse over links to read term descriptions.
GO:0003922   GMP synthase (glutamine-hydrolyzing) activity  
GO:0016462   pyrophosphatase activity  
GO:0005524   ATP binding  
GO:0003824   catalytic activity  
GO:0009435   NAD biosynthetic process  
GO:0006541   glutamine metabolic process  
GO:0006177   GMP biosynthetic process  
GO:0006164   purine nucleotide biosynthetic process  

Metabolic Pathways

Purine metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
18 569 1gpm (A) 5 525 54.00 0 1 1.59 -0.86
21 243 1wl8 (A) 1 188 38.00 0 1 0.76 -0.89
17 569 1gpm (A) 4 525 53.00 0 1 1.48158 0.01
22 238 2a9v (A) 3 184 35.00 0 1 0.703371 -0.8
22 569 5tw7 (A) 5 521 55.00 0 1 1.48209 -0.17
234 489 1ih8 (B) 1002 1271 19.00 0 0.98 0.548712 0.2

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_127152)

Species Accession Gene Product
Arabidopsis thaliana AT1G63660   putative GMP synthase / glutamine amidotransferase
Babesia bovis BBOV_IV009110   GMP synthase, putative
Brugia malayi Bm1_44235   GMP synthase
Candida albicans CaO19.12276   GMP synthase
Candida albicans CaO19.4813   GMP synthase
Caenorhabditis elegans CELE_M106.4   Protein GMPS-1, isoform C
Cryptosporidium hominis Chro.50499   burgundy CG9242-PA
Cryptosporidium parvum cgd5_4520   GMP synthase
Dictyostelium discoideum DDB_G0281551   GMP synthetase
Drosophila melanogaster Dmel_CG9242   burgundy
Escherichia coli b2507   GMP synthetase (glutamine aminotransferase)
Echinococcus granulosus EgrG_001198000   GMP synthase glutamine hydrolyzing
Echinococcus multilocularis EmuJ_001198000   GMP synthase (glutamine hydrolyzing)
Homo sapiens ENSG00000163655   guanine monphosphate synthase
Leishmania braziliensis LbrM.22.0110   GMP synthase, putative,glutamine amidotransferase, putative
Leishmania donovani LdBPK_220013.1   GMP synthase [glutamine-hydrolysing]
Leishmania infantum LinJ.22.0013   GMP synthase, putative,glutamine amidotransferase, putative
Leishmania major LmjF.22.0110   GMP synthase, putative,glutamine amidotransferase, putative
Leishmania mexicana LmxM.22.0110   GMP synthase, putative,glutamine amidotransferase, putative
Loa Loa (eye worm) LOAG_00204   hypothetical protein
Mycobacterium leprae ML0395   Probable GMP synthase [glutamine-hydrolyzing] GuaA (Glutamine amidotransferase) (GMP synthetase)
Mus musculus 229363   guanine monophosphate synthetase
Mycobacterium tuberculosis Rv3396c   Probable GMP synthase [glutamine-hydrolyzing] GuaA (glutamine amidotransferase) (GMP synthetase)
Mycobacterium ulcerans MUL_0913   GMP synthase
Neospora caninum NCLIV_030970   hypothetical protein
Oryza sativa 4345281   Os08g0326600
Plasmodium berghei PBANKA_1211000   GMP synthase [glutamine-hydrolyzing], putative
Plasmodium falciparum PF3D7_1012600   GMP synthase [glutamine-hydrolyzing]
Plasmodium knowlesi PKNH_0812400   GMP synthase [glutamine-hydrolyzing], putative
Plasmodium vivax PVX_094850   GMP synthetase, putative
Plasmodium yoelii PY03479   GMP synthetase
Saccharomyces cerevisiae YMR217W   GMP synthase (glutamine-hydrolyzing)
Schistosoma japonicum Sjp_0208860   ko:K01951 GMP synthase (glutamine-hydrolysing) [EC6.3.5.2], putative
Schistosoma mansoni Smp_063830   GMP synthetase
Schmidtea mediterranea mk4.038307.00  
Schmidtea mediterranea mk4.002352.00   GMP synthetase
Schmidtea mediterranea mk4.000132.06  
Trypanosoma brucei gambiense Tbg972.7.2290   GMP synthase, putative,glutamine amidotransferase, putative
Trypanosoma brucei Tb927.7.2100   GMP synthase [glutamine-hydrolysing]
Trypanosoma congolense TcIL3000_0_55050   GMP synthase [glutamine-hydrolysing]
Trypanosoma cruzi TcCLB.511807.110   GMP synthase [glutamine-hydrolysing]
Toxoplasma gondii TGME49_230450   bifunctional GMP synthase/glutamine amidotransferase protein
Theileria parva TP01_0862   GMP synthase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0443   GMP synthase

Essentiality

TGME49_230450 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.2100 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.2100 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.2100 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.2100 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2507 Escherichia coli essential goodall
PBANKA_1211000 Plasmodium berghei Slow plasmo
TGME49_230450 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.8

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum GMP synthase [glutamine-hydrolyzing] Compounds References
Mus musculus guanine monophosphate synthetase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0037 0.2717 0
0.00692925 0.693272 1
0.00692925 0.693272 1
0.0073 0.3767 1
0.0037 0.2717 0
0.0108 0.2533 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_230450 (Toxoplasma gondii), bifunctional GMP synthase/glutamine amidotransferase protein
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