Detailed view for LmjF.14.0240
Basic information
Leishmania major, hypothetical protein, conserved
Source Database / ID: TriTrypDB GeneDB
pI: 6.5962 |
Length (AA): 142 |
MW (Da): 15317 |
Paralog Number:
0
Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Network
Pfam domains
Gene Ontology
Metabolic Pathways
Structural information
Modbase 3D models:
There are 4 models calculated for this protein. More info on
these models, including the
models themselves is available at:
Modbase
| Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
|---|---|---|---|---|---|---|---|---|---|
| 3 | 139 | 1y23 (A) | 5 | 143 | 39.00 | 0 | 1 | 1.45 | -0.35 |
| 4 | 108 | 1xqu (A) | 6 | 111 | 38.00 | 0 | 1 | 1.28 | -0.92 |
| 1 | 141 | 3ksv (A) | 1 | 141 | 99.99 | 0 | 1 | 2.12726 | -0.4 |
| 5 | 105 | 2eo4 (A) | 1 | 101 | 34.00 | 0.0000000021 | 1 | 1.23257 | -0.87 |
Help me make sense of these data.
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
- 3KSV:
-
Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
Expression
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Upper 60-80% percentile | amastigotes, metacyclic. | Fernandes MC |
-
Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.
Orthologs
Ortholog group members (OG5_126794)
| Species | Accession | Gene Product |
|---|---|---|
| Arabidopsis thaliana | AT5G48545 | protein histidine triad nucleotide-binding 3 |
| Arabidopsis thaliana | AT1G31160 | histidine triad nucleotide-binding 2 protein |
| Arabidopsis thaliana | AT3G56490 | HIS triad family protein 3 |
| Babesia bovis | BBOV_III011870 | HIT domain containing protein |
| Brugia malayi | Bm1_56730 | Hypothetical HIT-like protein F21C3.3 |
| Candida albicans | CaO19.2341 | potential histidine triad nucleotide-binding protein similar to S. cerevisiae HNT1 (YDL125C) Kin28p-interacting adenosine 5'-mon |
| Candida albicans | CaO19.9877 | similar to the histidine triad superfamily of nucleotide-binding proteins |
| Caenorhabditis elegans | CELE_F21C3.3 | Protein HINT-1 |
| Cryptosporidium hominis | Chro.10184 | hypothetical protein |
| Cryptosporidium parvum | cgd1_1600 | histidine triad (HIT) family zinc binding protein |
| Chlamydia trachomatis | CT_385 | Hit family hydrolase |
| Dictyostelium discoideum | DDB_G0289439 | histidine triad family protein |
| Dictyostelium discoideum | DDB_G0289391 | histidine triad family protein |
| Drosophila melanogaster | Dmel_CG2862 | CG2862 gene product from transcript CG2862-RA |
| Escherichia coli | b1103 | purine nucleoside phosphoramidase, dadA activator protein |
| Echinococcus granulosus | EgrG_000597600 | serine:threonine protein kinase KDX1 |
| Entamoeba histolytica | EHI_093910 | hypothetical protein, conserved |
| Echinococcus multilocularis | EmuJ_000597600 | serine:threonine protein kinase KDX1 |
| Giardia lamblia | GL50803_4156 | HIT family protein |
| Homo sapiens | 3094 | histidine triad nucleotide binding protein 1 |
| Homo sapiens | ENSG00000137133 | histidine triad nucleotide binding protein 2 |
| Leishmania braziliensis | LbrM.14.0240 | hypothetical protein, conserved |
| Leishmania donovani | LdBPK_140240.1 | HIT domain containing protein, putative |
| Leishmania infantum | LinJ.14.0240 | hypothetical protein, conserved |
| Leishmania major | LmjF.14.0240 | hypothetical protein, conserved |
| Leishmania mexicana | LmxM.14.0240 | hypothetical protein, conserved |
| Loa Loa (eye worm) | LOAG_07417 | hypothetical protein |
| Mycobacterium leprae | ML2237 | conserved hypothetical protein (HIT family) |
| Mus musculus | ENSMUSG00000028470 | histidine triad nucleotide binding protein 2 |
| Mus musculus | ENSMUSG00000020267 | histidine triad nucleotide binding protein 1 |
| Mycobacterium tuberculosis | Rv1262c | Hypothetical hit-like protein |
| Mycobacterium tuberculosis | Rv0759c | Conserved hypothetical protein |
| Mycobacterium ulcerans | MUL_0465 | hypothetical protein |
| Mycobacterium ulcerans | MUL_4479 | Hit-like protein |
| Neospora caninum | NCLIV_018300 | HIT family protein, related |
| Oryza sativa | 4351835 | Os12g0233300 |
| Oryza sativa | 9270866 | Os11g0295000 |
| Oryza sativa | 4332685 | Os03g0322500 |
| Plasmodium berghei | PBANKA_0713300 | protein kinase c inhibitor-like protein, putative |
| Plasmodium falciparum | PF3D7_0817500 | protein kinase c inhibitor-like protein, putative |
| Plasmodium knowlesi | PKNH_0502900 | protein kinase c inhibitor-like protein, putative |
| Plasmodium vivax | PVX_089540 | protein kinase c inhibitor-like protein, putative |
| Plasmodium yoelii | PY05168 | putative protein kinase C interacting protein 1 |
| Saccharomyces cerevisiae | YDL125C | Hnt1p |
| Schistosoma japonicum | Sjp_0218600 | ko:K06069 atypical protein kinase C, putative |
| Schistosoma mansoni | Smp_048650 | histidine triad (hit) protein |
| Schmidtea mediterranea | mk4.003135.01 | Histidine triad nucleotide-binding protein 2, mitochondrial |
| Schmidtea mediterranea | mk4.003480.02 | Histidine triad nucleotide-binding protein 2, mitochondrial |
| Schmidtea mediterranea | mk4.014420.00 | Histidine triad nucleotide-binding protein 2, mitochondrial |
| Trypanosoma brucei gambiense | Tbg972.7.5070 | adenosine 5-monophosphoramidase, putative |
| Trypanosoma brucei | Tb927.7.4480 | adenosine 5'-monophosphoramidase, putative |
| Trypanosoma cruzi | TcCLB.506605.59 | adenosine 5'-monophosphoramidase, putative |
| Trypanosoma cruzi | TcCLB.511239.49 | adenosine 5'-monophosphoramidase, putative |
| Toxoplasma gondii | TGME49_243580 | Hit family protein involved in cell-cycle regulation, putative |
| Theileria parva | TP01_0012 | protein kinase C interacting protein 1, putative |
| Wolbachia endosymbiont of Brugia malayi | Wbm0240 | HIT family hydrolase |
Essentiality
| Gene/Ortholog | Organism | Phenotype | Source Study |
|---|---|---|---|
| mtu1283 | Mycobacterium tuberculosis | non-essential | nmpdr |
| Tb927.7.4480 | Trypanosoma brucei | significant loss of fitness in bloodstream forms (3 days) | alsford |
| Tb927.7.4480 | Trypanosoma brucei | no significant loss or gain of fitness in bloodstream forms (6 days) | alsford |
| Tb927.7.4480 | Trypanosoma brucei | no significant loss or gain of fitness in procyclic forms | alsford |
| Tb927.7.4480 | Trypanosoma brucei | no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms | alsford |
| b1103 | Escherichia coli | non-essential | goodall |
| PBANKA_0713300 | Plasmodium berghei | Dispensable | plasmo |
| TGME49_243580 | Toxoplasma gondii | Probably non-essential | sidik |
-
blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930 -
shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan -
neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs -
wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170 -
nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource -
keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection -
gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84 -
yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database -
alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
Phenotypes and Validation (curated)
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
Annotated validation
Associated compounds / Druggability
Known modulators for this target
Predicted associations
By orthology with druggable targets
By sequence similarity to non orthologous druggable targets
Obtained from network model
Assayability
Assay information
Reagent availability
- Lmaj00884AAA;
-
Type Source Notes soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Lmaj00884; Recombinant protein: full-length; Source: L major; hypothetical protein, conserved ;
Bibliographic References