Detailed view for LmjF.13.1210

Basic information

TDR Targets ID: 28267
Leishmania major, nucleobase transporter
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1783 | Length (AA): 501 | MW (Da): 54604 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: Y | Predicted trans-membrane segments: 11

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01733   Nucleoside transporter

Gene Ontology

Mouse over links to read term descriptions.
GO:1901642   GO:nucleoside transmembrane transport  

GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0005337   nucleoside transmembrane transporter activity  
GO:0006810   transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
26 425 1pw4 (A) 32 436 11.00 0.0000000001 0.04 0.86 -0.48
56 125 2ft0 (A) 9 88 36.00 0.53 0.23 0.0143206 3.01

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_132650)

Species Accession Gene Product
Arabidopsis thaliana AT1G61630   equilibrative nucleoside transporter 7
Caenorhabditis elegans CELE_C47A4.2   Protein ENT-6, isoform A
Leishmania braziliensis LbrM.13.0990   nucleobase transporter
Leishmania donovani LdBPK_131110.1   nucleobase transporter
Leishmania infantum LinJ.13.1110   nucleobase transporter
Leishmania major LmjF.13.1210   nucleobase transporter
Leishmania mexicana LmxM.13.1210   nucleobase transporter
Trypanosoma brucei gambiense Tbg972.11.4110   nucleobase/nucleoside transporter 8.1,nucleobase transporter
Trypanosoma brucei gambiense Tbg972.11.4150   nucleobase/nucleoside transporter, putative
Trypanosoma brucei gambiense Tbg972.11.4130   nucleobase transporter, putative
Trypanosoma brucei gambiense Tbg972.11.4120   nucleobase/nucleoside transporter, putative
Trypanosoma brucei Tb11.v5.1049   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma brucei Tb927.11.3630   nucleobase/nucleoside transporter 8.1
Trypanosoma brucei Tb927.11.3620   nucleobase/nucleoside transporter 8.1
Trypanosoma brucei Tb11.v5.1052   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma brucei Tb11.v5.1054   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma brucei Tb11.v5.1051   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma brucei Tb11.v5.1044   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma congolense TcIL3000_0_55300   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma congolense TcIL3000.11.3570   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma congolense TcIL3000.11.3590   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma congolense TcIL3000_0_59770   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma congolense TcIL3000_0_17680   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma congolense TcIL3000_0_48120   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma congolense TcIL3000_0_38110   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma congolense TcIL3000.11.3580   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma congolense TcIL3000_0_58780   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma congolense TcIL3000_0_59040   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma congolense TcIL3000_0_12860   nucleobase/nucleoside transporter 8.1, putative
Trypanosoma cruzi TcCLB.509683.110   nucleobase transporter, putative
Trypanosoma cruzi TcCLB.511051.30   nucleobase transporter, putative

Essentiality

LmjF.13.1210 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.1100 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.1100 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.1100 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.1100 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Equilibrative nucleoside transporter 1 457 aa 23.3% 529 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LmjF.13.1210 (Leishmania major), nucleobase transporter
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