Detailed view for LmjF.36.6300

Basic information

TDR Targets ID: 28815
Leishmania major, glucose transporter, lmgt1

Source Database / ID: TriTrypDB / LmjF.36.6300  GeneDB / LmjF.36.6300

pI: 7.2872 | Length (AA): 653 | MW (Da): 70734 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: ND

Pfam domains

PF00083   Sugar (and other) transporter

Gene Ontology

Mouse over links to read term descriptions.
GO:0022857   transmembrane transporter activity  
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0005215   transporter activity  
GO:0022891   substrate-specific transmembrane transporter activity  
GO:0055085   transmembrane transport  
GO:0006810   transport  

Network

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models: 1

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
197 632 1pw4 (A) 21 449 11.00 0 0.21 0.73 -0.9

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_129633)
Species Accession Gene Product
Dictyostelium discoideum DDB_G0286151   hypothetical protein
Dictyostelium discoideum DDB_G0281155   sugar transporter family protein
Dictyostelium discoideum DDB_G0277773   solute carrier family 2 member protein
Dictyostelium discoideum DDB_G0292814   calmodulin-binding protein
Leishmania braziliensis LbrM.35.6480   lmgt2, putative
Leishmania braziliensis LbrM.35.6490   glucose transporter, lmgt2
Leishmania braziliensis LbrM.33.0290   glucose transporter/membrane transporter D2, putative
Leishmania donovani LdBPK_366560.1   glucose transporter 1
Leishmania donovani LdBPK_366540.1   glucose transporter 3, putative
Leishmania donovani LdBPK_330310.1   glucose transporter/membrane transporter D2, putative
Leishmania infantum LinJ.33.0310   glucose transporter/membrane transporter D2, putative
Leishmania infantum LinJ.36.6540   glucose transporter, lmgt3
Leishmania infantum LinJ.36.6560   glucose transporter, lmgt1
Leishmania infantum LinJ.36.6550   glucose transporter, lmgt2
Leishmania major LmjF.33.0290   glucose transporter/membrane transporter D2, putative
Leishmania major LmjF.36.6300 this record   glucose transporter, lmgt1
Leishmania major LmjF.36.6280   glucose transporter, lmgt3
Leishmania major LmjF.36.6290   glucose transporter, lmgt2
Leishmania mexicana LmxM.32.0290   glucose transporter/membrane transporter D2, putative
Leishmania mexicana LmxM.36.6300   glucose transporter, lmgt1
Leishmania mexicana LmxM.36.6280   glucose transporter, lmgt3
Leishmania mexicana LmxM.36.6290   glucose transporter, lmgt2
Trypanosoma brucei gambiense Tbg972.10.10290   hexose transporter,glucose transporter 1B, putative
Trypanosoma brucei gambiense Tbg972.4.2260   glucose transporter, putative
Trypanosoma brucei gambiense Tbg972.10.10310   glucose transporter
Trypanosoma brucei gambiense Tbg972.10.10300   glucose transporter,glucose transporter 2A, putative
Trypanosoma brucei gambiense Tbg972.10.10280   hexose transporter,glucose transporter 1B
Trypanosoma brucei Tb11.v5.0307   THT1 - hexose transporter, putative
Trypanosoma brucei Tb927.10.8500   glucose transporter, putative
Trypanosoma brucei Tb927.10.8490   glucose transporter, putative
Trypanosoma brucei Tb927.10.8510   glucose transporter, putative
Trypanosoma brucei Tb927.10.8460   glucose transporter, putative
Trypanosoma brucei Tb927.10.8480   glucose transporter, putative
Trypanosoma brucei Tb927.10.8520   glucose transporter, putative
Trypanosoma brucei Tb927.10.8530   glucose transporter 2A
Trypanosoma brucei Tb11.v5.0310   THT1 - hexose transporter, putative
Trypanosoma brucei Tb11.v5.0330   THT1 - hexose transporter, putative
Trypanosoma brucei Tb927.4.2290   glucose transporter, putative
Trypanosoma brucei Tb927.10.8450   glucose transporter 1E
Trypanosoma brucei Tb927.10.8470   glucose transporter, putative
Trypanosoma congolense TcIL3000_4_2100   glucose transporter, putative
Trypanosoma congolense TcIL3000_10_7320   glucose transporter 2A, putative
Trypanosoma congolense TcIL3000_10_7310   glucose transporter, putative
Trypanosoma cruzi TcCLB.511041.40   hexose transporter, putative
Trypanosoma cruzi TcCLB.506355.10   hexose transporter
Trypanosoma cruzi TcCLB.508551.39   hexose transporter, putative
Trypanosoma cruzi TcCLB.506355.5   hexose transporter, putative
Trypanosoma cruzi TcCLB.508551.30   hexose transporter, putative
Trichomonas vaginalis TVAG_247420   glucose transporter, putative
Trichomonas vaginalis TVAG_039990   sugar transporter, putative
Trichomonas vaginalis TVAG_040000   glucose transporter, putative
Trichomonas vaginalis TVAG_039960   sugar transporter, putative
Trichomonas vaginalis TVAG_039970   conserved hypothetical protein
Trichomonas vaginalis TVAG_037050   sugar transporter, putative
Trichomonas vaginalis TVAG_495680   conserved hypothetical protein

Essentiality

LmjF.36.6300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.2290 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.2290 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.2290 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.2290 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.8530 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.8530 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.8530 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.8530 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.8440 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.8440 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.8440 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.8440 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford

Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
transporter activity (GO:0005215) decreased (PATO:0000468) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: targeted disruption of the LmGT locus that encompasses the LmGT1, LmGT2, and LmGT3 genes led to cells that expressed no glucose transport activity; . References: 12651954
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: targeted disruption of the LmGT locus that encompasses the LmGT1, LmGT2, and LmGT3 genes led to cells with growth reduced to the same level as cells grown without glucose; . References: 12651954
viability (PATO:0000169) decreased (PATO:0000468) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: targeted disruption of the LmGT locus that encompasses the LmGT1, LmGT2, and LmGT3 genes led to cells with reduced infectivity and ability to survive in macrophages; . References: 12651954 16707495
size (PATO:0000117) decreased (PATO:0000468) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: targeted disruption of the LmGT locus that encompasses the LmGT1, LmGT2, and LmGT3 genes led to cells with size; . References: 17306380
response to oxidative stress (GO:0006979) abnormal (PATO:0000460) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: targeted disruption of the LmGT locus that encompasses the LmGT1, LmGT2, and LmGT3 genes led to cells more sensitive to oxidative stress; . References: 17306380

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
 
In any case, if you have information about papers containing relevant validation data for this target, please log in using your TDR Targets username and password and send them to us using the corresponding form in this page (only visible to registered users) or contact us.

Annotated validation

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Curated from literature

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Leishmania mexicana glucose transporter, lmgt2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

3 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.36.6300 (Leishmania major), glucose transporter, lmgt1
Title for this comment
Comment

© 2013 – 2018 Trypanosomatics Lab | IIBIO - UNSAM
© 2005 – 2012 The TDR Drug Targets Network (WHO/TDR)

TDR Targets Development Release v6, Revision: 1335 (10.Aug.2018)
Contact Us: <info at tdrtargets org>