Detailed view for LmjF.34.4330

Basic information

TDR Targets ID: 29515
Leishmania major, quinonoid dihydropteridine reductase
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2273 | Length (AA): 229 | MW (Da): 23581 | Paralog Number: 6

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00106   short chain dehydrogenase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0016491   oxidoreductase activity  
GO:0008152   metabolic process  

Metabolic Pathways

Folate biosynthesis (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 229 1geg (A) 3 255 18.00 0 1 1.1 -1.02
2 228 1dhr () 7 236 41.00 0 1 1.5 -1.47
4 222 1ooe (A) 6 228 44.00 0 1 1.6 -1.31
2 225 1ooe (A) 4 231 42.00 0 1 1.52637 -0.76
2 228 3orf (A) 3 229 36.00 0 1 1.49017 -0.96
2 228 1dhr (A) 7 236 41.00 0 1 1.55047 -0.92

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_131023)

Species Accession Gene Product
Brugia malayi Bm1_25735   dihydropteridine reductase-like
Caenorhabditis elegans CELE_T03F6.1   Protein QDPR-1
Dictyostelium discoideum DDB_G0272684   dihydropteridine reductase
Drosophila melanogaster Dmel_CG4665   Dihydropteridine reductase
Echinococcus granulosus EgrG_000819200   NADP binding domain
Echinococcus multilocularis EmuJ_000819200   NAD(P) binding domain
Homo sapiens ENSG00000151552   quinoid dihydropteridine reductase
Leishmania braziliensis LbrM.20.3970   quinonoid dihydropteridine reductase
Leishmania donovani LdBPK_044270.1   hypothetical protein, conserved
Leishmania infantum LinJ.34.4270   quinonoid dihydropteridine reductase
Leishmania major LmjF.34.4390   quinonoid dihydropteridine reductase
Leishmania major LmjF.34.4330   quinonoid dihydropteridine reductase
Leishmania major LmjF.34.4450   quinonoid dihydropteridine reductase
Leishmania major LmjF.34.4480   quinonoid dihydropteridine reductase
Leishmania major LmjF.34.4420   quinonoid dihydropteridine reductase
Leishmania major LmjF.34.4510   quinonoid dihydropteridine reductase
Leishmania major LmjF.34.4360   quinonoid dihydropteridine reductase
Leishmania mexicana LmxM.33.4330   quinonoid dihydropteridine reductase
Loa Loa (eye worm) LOAG_02547   dihydropteridine reductase-PC
Mus musculus ENSMUSG00000015806   quinoid dihydropteridine reductase
Neospora caninum NCLIV_014630   Novel protein similar to quinoid dihydropteridine reductase (QDPR, zgc:112405), related
Schistosoma japonicum Sjp_0002580   ko:K00357 dihydropteridine reductase [EC1.5.1.34], putative
Schistosoma mansoni Smp_086210   dihydropteridine reductase
Schmidtea mediterranea mk4.020580.00  
Schmidtea mediterranea mk4.019996.00  
Schmidtea mediterranea mk4.001430.06   Dihydropteridine reductase
Trypanosoma cruzi TcCLB.510689.20   quinonoid dihydropteridine reductase, putative
Toxoplasma gondii TGME49_285750   6,7-dihydropteridine reductase

Essentiality

LmjF.34.4330 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_285750 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.8

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens quinoid dihydropteridine reductase Compounds References
Rattus norvegicus Dihydropteridine reductase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0157 0.5 0.5
0.0084 0.4283 0.4047
0.0102 0.7451 0.5
0.0196 0.8679 0.5
0.0163 0.2942 0
0.0202 1 0.5
0.0113 1 1
0.1222 1 1
0.0056 1 1
0.0053 1 1
0.109 1 1
0.025 0.3237 1
0.0202 1 0.5
0.0202 0.4841 1
0.11 0.3004 1
0.0163 0.2957 0
0.0968 1 1
0.0335 0.3117 0
0.0056 0.875 1
0.033 0.3239 0.844
0.0602 1 1
0.1061 0.5937 1
0.016 0.577 1
0.0004 0.5 0.5
0.1061 0.5937 1
0.0082 0.3198 0.5
0.0073 0.3811 1
0.0088 0.6544 1
0.007 1 1
0.0009 0.5 0.5
0.0123 0.5 0.5
0.0202 1 0.5
0.0019 0.4322 1
0.0046 0.3082 0
0.1276 1 1
0.0971 1 1
0.0009 0.5 0.5
0.0114 1 1
0.0021 0.5 0.5
0.0064 0.3508 0.5
0.0964 1 1
0.0336 1 1
0.0165 0.2952 0
0.0267 0.5226 0.7253
0.0089 0.5 0.5
0.1054 1 1
0.0053 1 1
0.004 0.5 0.5
0.0066 0.3586 0.5
0.0165 1 1
0.1061 0.5937 1
0.0004 0.5 0.5
0.0045 1 1
0.0089 0.5 0.5
0.0165 0.2952 0
0.0073 0.2873 0.4086
0.0058 0.3931 1
0.0339 1 0.5
0.0097 0.4304 1
0.11 0.3004 1
0.1061 0.5937 1
0.0919 0.9691 1
0.0016 0.5 0.5
0.0408 0.5144 1
0.0009 0.5 0.5
0.0196 0.8679 0.5
0.008 0.5 0.5
0.0009 0.5 0.5
0.0334 0.3139 0.8444
0.0004 0.5 0.5
0.0968 1 1
0.0088 1 1
0.0076 1 1
0.0068 1 1
0.0602 1 1
0.016 0.577 1
0.051 0.3375 0
0.089 0.9299 1
0.1061 0.5937 1
0.007 0.2916 1
0.0795 0.3067 1
0.007 1 1
0.0246 0.2963 0.3142
0.1096 1 1
0.0004 0.5 0.5
0.0004 0.5 0.5
0.0108 0.5 0.5
0.0171 0.4063 0.5
0.0171 0.4063 0.5
0.0169 0.2804 1
0.0021 0.3321 0.5
0.0039 1 1
0.0005 0.5 0.5
0.0013 0.5 0.5
0.0009 0.5 0.5
0.0013 1 0.5
0.0408 0.5144 1
0.0009 0.5 0.5
0.0536 1 0.5
0.0955 1 1
0.051 0.3391 0.4263
0.0093 0.5 0.5
0.011 0.3568 0.5973
0.0402 1 1
0.0009 1 0.5
0.0106 0.5 0.5
0.0162 0.3549 1
0.0114 1 1
0.0058 1 1
0.0012 0.5 0.5
0.0035 1 1
0.0027 0.5 0.5
0.0248 0.3101 0.6892
0.007 1 1
0.0073 0.3811 1
0.0119 0.2746 1
0.0073 0.3141 1
0.0068 1 1
0.0131 0.5 0.5
0.0118 1 1
0.007 0.2916 1
0.0162 1 1
0.0106 1 1
0.1061 0.5937 1
0.0202 1 0.5
0.0202 1 0.5
0.0021 0.3321 0.5
0.0202 1 0.5
0.0082 0.3198 0.5
0.0068 0.2585 0.5
0.0132 0.3658 1
0.051 0.2514 0.4262
0.051 0.3411 0.4303
0.0333 0.3314 0.2086
0.0336 1 1
0.0032 1 1
0.0004 0.5 0.5
0.0071 0.3141 0
0.0097 1 1
0.1061 0.5937 1
0.0009 0.5 0.5
0.0027 0.5 0.5
0.0082 1 0.5
0.0163 0.2727 0
0.0333 0.3365 0
0.0196 0.8679 0.5
0.0244 0.2867 0.6732
0.0106 1 0.5
0.0073 0.4546 1
0.0009 0.7374 0.5
0.0631 1 0.5
0.0218 0.5 0.5
0.0087 0.5 0.5
0.0085 1 1
0.007 0.2916 1
0.0009 0.5 0.5
0.0005 0.5 0.5
0.0169 0.4844 1
0.025 0.3237 1
0.0106 0.2872 1
0.0026 0.5 0.5
0.0067 0.3468 1
0.0222 0.5 0.5
0.0057 0.284 0.6352
0.0202 1 0.5
0.0004 0.5 0.5
0.025 0.3237 1
0.0408 0.5144 1
0.0012 0.5 0.5

Assayability

Assay information

  • Assay for Dihydropteridine Reductase (1.6.99.7 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.34.4330 (Leishmania major), quinonoid dihydropteridine reductase
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