Detailed view for TcCLB.508301.20

Basic information

TDR Targets ID: 30093
Trypanosoma cruzi, mitochondrial processing peptidase, beta subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6771 | Length (AA): 272 | MW (Da): 30793 | Paralog Number: 3

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05193   Peptidase M16 inactive domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008270   zinc ion binding  
GO:0004222   metalloendopeptidase activity  
GO:0003824   catalytic activity  
GO:0046872   metal ion binding  
GO:0006508   proteolysis  

Metabolic Pathways

Oxidative phosphorylation (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 272 1ezv (A) 205 456 21.00 0 1 1.17 0.08
1 265 1hr6 (B) 208 458 27.00 0 1 1.3 -0.39
1 271 3tgu (A) 195 443 31.00 0 1 1.26512 0.16
85 252 3ami (A) 279 446 11.00 0 0.04 0.732647 -0.35
137 193 5b8d (A) 1153 1207 33.00 0.54 0.02 0.411359 0.95

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127023)

Species Accession Gene Product
Arabidopsis thaliana AT3G02090   probable mitochondrial-processing peptidase subunit beta
Babesia bovis BBOV_IV001260   mitochondrial processing peptidase beta subunit
Brugia malayi Bm1_07510   mitochondria processing peptidase subunit beta, putative
Candida albicans CaO19.3026   beta-mitochondrial processing peptidase
Candida albicans CaO19.10544   beta-mitochondrial processing peptidase
Caenorhabditis elegans CELE_ZC410.2   Protein MPPB-1
Cryptosporidium hominis Chro.50031   mitochondrial processing peptidase beta subunit
Cryptosporidium parvum cgd5_3400   mitochondrial processing peptidase beta subunit
Dictyostelium discoideum DDB_G0288777   mitochondrial processing peptidase beta subunit
Drosophila melanogaster Dmel_CG3731   CG3731 gene product from transcript CG3731-RA
Echinococcus granulosus EgrG_000113700   mitochondrial processing peptidase beta subunit
Entamoeba histolytica EHI_098460   hypothetical protein
Echinococcus multilocularis EmuJ_000113700   mitochondrial processing peptidase beta subunit
Homo sapiens ENSG00000010256   ubiquinol-cytochrome c reductase core protein I
Homo sapiens 9512   peptidase (mitochondrial processing) beta
Leishmania braziliensis LbrM.34.1300   mitochondrial processing peptidase, beta subunit, putative,metallo-peptidase, Clan ME, Family M16
Leishmania donovani LdBPK_010670.1   mitochondrial-processing peptidase subunit beta, putative
Leishmania donovani LdBPK_351390.1   mitochondrial processing peptidase, beta subunit, putative
Leishmania infantum LinJ.01.0670   mitochondrial processing peptide beta subunit, putative,metallo-peptidase, Clan ME, Family M16
Leishmania infantum LinJ.35.1390   mitochondrial processing peptidase, beta subunit, putative,metallo-peptidase, Clan ME, Family M16
Leishmania major LmjF.35.1380   mitochondrial processing peptidase, beta subunit, putative,metallo-peptidase, Clan ME, Family M16
Leishmania mexicana LmxM.01.0650   mitochondrial processing peptide beta subunit, putative,metallo-peptidase, Clan ME, Family M16
Leishmania mexicana LmxM.34.1380   mitochondrial processing peptidase, beta subunit, putative,metallo-peptidase, Clan ME, Family M16
Loa Loa (eye worm) LOAG_08266   processing peptidase subunit beta
Mycobacterium leprae ML0855   PROBABLE ZINC PROTEASE PEPR
Mus musculus ENSMUSG00000025651   ubiquinol-cytochrome c reductase core protein 1
Mus musculus ENSMUSG00000029017   peptidase (mitochondrial processing) beta
Mycobacterium tuberculosis Rv2782c   Probable zinc protease PepR
Neospora caninum NCLIV_050470   hypothetical protein
Oryza sativa 4332040   Os03g0212700
Plasmodium berghei PBANKA_0834400   mitochondrial-processing peptidase subunit beta, putative
Plasmodium falciparum PF3D7_0933600   mitochondrial-processing peptidase subunit beta, putative
Plasmodium knowlesi PKNH_0732300   mitochondrial-processing peptidase subunit beta, putative
Plasmodium vivax PVX_087035   mitochondrial-processing peptidase subunit beta, putative
Plasmodium yoelii PY01832   mitochondrial processing peptidase beta subunit
Saccharomyces cerevisiae YLR163C   Mas1p
Schistosoma japonicum Sjp_0204880   ko:K01412 mitochondrial processing peptidase [EC3.4.24.64], putative
Schistosoma mansoni Smp_009650.2   mitochondrial processing peptidase beta-subunit (M16 family)
Schistosoma mansoni Smp_009650.1   mitochondrial processing peptidase beta-subunit (M16 family)
Schmidtea mediterranea mk4.000731.00   Mitochondrial-processing peptidase subunit beta
Schmidtea mediterranea mk4.001322.14   Mitochondrial-processing peptidase subunit beta
Trypanosoma brucei gambiense Tbg972.9.2390   mitochondrial processing peptide beta subunit, putative,metallo-peptidase, Clan ME, Family M16
Trypanosoma brucei gambiense Tbg972.5.1430   mitochondrial processing peptidase, beta subunit, putative,metallo-peptidase, Clan ME, Family M16, putative
Trypanosoma brucei Tb927.9.4520   Mitochondrial-processing peptidase subunit beta
Trypanosoma brucei Tb927.5.1060   mitochondrial processing peptidase, beta subunit, putative
Trypanosoma congolense TcIL3000_5_820   mitochondrial processing peptidase, beta subunit, putative
Trypanosoma cruzi TcCLB.511585.80   metallo-peptidase, Clan ME, Family M16
Trypanosoma cruzi TcCLB.510155.80   mitochondrial-processing peptidase subunit beta, putative
Trypanosoma cruzi TcCLB.508301.20   mitochondrial processing peptidase, beta subunit, putative
Trypanosoma cruzi TcCLB.511181.50   mitochondrial processing peptidase, beta subunit, putative
Theileria parva TP01_0151   biquinol-cytochrome C reductase complex core protein I, mitochondrial precursor, putative
Trichomonas vaginalis TVAG_233350   Clan ME, family M16, insulinase-like metallopeptidase
Wolbachia endosymbiont of Brugia malayi Wbm0221   Zn-dependent peptidase

Essentiality

TcCLB.508301.20 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu2829 Mycobacterium tuberculosis non-essential nmpdr
Tb09.160.3110 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.3110 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.3110 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.3110 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.5.1060 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.1060 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.1060 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.5.1060 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_ZC410.2 Caenorhabditis elegans embryonic lethal wormbase
YLR163C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0834400 Plasmodium berghei Slow plasmo
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

6 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.508301.20 (Trypanosoma cruzi), mitochondrial processing peptidase, beta subunit, putative
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