Detailed view for TcCLB.504147.20

Basic information

TDR Targets ID: 30568
Trypanosoma cruzi, 60S ribosomal protein L18, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 12.1841 | Length (AA): 193 | MW (Da): 21677 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF17135   Ribosomal protein 60S L18 and 50S L18e

Gene Ontology

Mouse over links to read term descriptions.
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
21 147 1vq8 (O) 2 115 29.00 0 1 1.08 -1.46
21 147 1vq8 (O) 2 115 30.00 0 0.85 0.913131 0.14
22 147 1vq8 (O) 3 115 28.00 0 1 0.95225 -0.79
131 191 1dt9 (A) 144 203 18.00 0 0.04 0.452462 -0.09

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 5T5H:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127236)

Species Accession Gene Product
Arabidopsis thaliana AT5G27850   60S ribosomal protein L18-3
Arabidopsis thaliana AT2G47570   putative 60S ribosomal protein L18-1
Arabidopsis thaliana AT3G05590   60S ribosomal protein L18-2
Babesia bovis BBOV_III004640   60S ribosomal protein L18, putative
Brugia malayi Bm1_29215   60S ribosomal protein L18
Candida albicans CaO19.5982   similar to C terminus of S. cerevisiae RPL18A (YOL120C) and RPL18B (YOL120C)
Candida albicans CaO19.13403   similar to C terminus of S. cerevisiae RPL18A (YOL120C) and RPL18B (YOL120C)
Caenorhabditis elegans CELE_Y45F10D.12   Protein RPL-18
Cryptosporidium hominis Chro.40062   eukaryotic ribosomal protein L18
Cryptosporidium parvum cgd4_470   60S ribosomal protein L18
Dictyostelium discoideum DDB_G0279997   S60 ribosomal protein L18
Drosophila melanogaster Dmel_CG8615   Ribosomal protein L18
Echinococcus granulosus EgrG_001047000   60S ribosomal protein L18
Entamoeba histolytica EHI_187740   ribosomal protein L18, putative
Entamoeba histolytica EHI_098470   ribosomal protein L18, putative
Entamoeba histolytica EHI_079190   ribosomal protein L18, putative
Echinococcus multilocularis EmuJ_001047000   60S ribosomal protein L18
Giardia lamblia GL50803_11950   Ribosomal protein L18
Homo sapiens ENSG00000063177   ribosomal protein L18
Leishmania braziliensis LbrM.35.4750   60S ribosomal protein L18, putative
Leishmania braziliensis LbrM.13.0380   60S ribosomal protein L18, putative
Leishmania donovani LdBPK_364730.1   60S ribosomal protein L18, putative
Leishmania infantum LinJ.36.4730   60S ribosomal protein L18, putative
Leishmania infantum LinJ.13.0450   60S ribosomal protein L18, putative
Leishmania major LmjF.36.4510   60S ribosomal protein L18, putative
Leishmania major LmjF.13.0560   60S ribosomal protein L18, putative
Leishmania mexicana LmxM.13.0560   60S ribosomal protein L18, putative
Leishmania mexicana LmxM.36.4510   60S ribosomal protein L18, putative
Loa Loa (eye worm) LOAG_06664   hypothetical protein
Mus musculus 101055744   predicted gene, 29720
Mus musculus ENSMUSG00000059070   ribosomal protein L18
Neospora caninum NCLIV_064700   Ribosomal protein L18, related
Oryza sativa 4337849   Os05g0155100
Oryza sativa 4344265   Os07g0674700
Oryza sativa 4332793   Os03g0341100
Plasmodium berghei PBANKA_1354500   60S ribosomal protein L18-2, putative
Plasmodium falciparum PF3D7_1341300   60S ribosomal protein L18-2, putative
Plasmodium knowlesi PKNH_1260000   60S ribosomal subunit porotein L18, putative
Plasmodium vivax PVX_082970   60S ribosomal protein L18, putative
Plasmodium yoelii PY01390   Eukaryotic ribosomal protein L18, putative
Saccharomyces cerevisiae YOL120C   ribosomal 60S subunit protein L18A
Saccharomyces cerevisiae YNL301C   ribosomal 60S subunit protein L18B
Schistosoma japonicum Sjp_0202460   ko:K02883 large subunit ribosomal protein L18e, putative
Schistosoma mansoni Smp_031570   60S ribosomal protein L18
Schmidtea mediterranea mk4.007797.04   60S ribosomal protein L18
Trypanosoma brucei gambiense Tbg972.11.4920   60S ribosomal protein L18, putative
Trypanosoma brucei gambiense Tbg972.10.12050   60S ribosomal protein L18, putative
Trypanosoma brucei Tb927.11.4300   60S ribosomal protein L18, putative
Trypanosoma brucei Tb927.10.9880   60S ribosomal protein L18, putative
Trypanosoma congolense TcIL3000_0_41290   60S ribosomal protein L18, putative
Trypanosoma congolense TcIL3000_10_8490   60S ribosomal protein L18, putative
Trypanosoma cruzi TcCLB.506181.50   60S ribosomal protein L18, putative
Trypanosoma cruzi TcCLB.503395.40   60S ribosomal protein L18, putative
Trypanosoma cruzi TcCLB.504147.20   60S ribosomal protein L18, putative
Toxoplasma gondii TGME49_300000   ribosomal protein RPL18
Theileria parva TP02_0801   60S ribosomal protein L18, putative
Trichomonas vaginalis TVAG_395470   60S ribosomal protein L18, putative
Trichomonas vaginalis TVAG_401590   60S ribosomal protein L18, putative
Trichomonas vaginalis TVAG_482430   60S ribosomal protein L18, putative
Trichomonas vaginalis TVAG_121100   60S ribosomal protein L18, putative
Trichomonas vaginalis TVAG_426320   60S ribosomal protein L18, putative
Trichomonas vaginalis TVAG_243990   60S ribosomal protein L18, putative

Essentiality

TcCLB.504147.20 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.9880 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.9880 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.9880 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.9880 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.02.1840 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.1840 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.1840 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.1840 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y45F10D.12 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y45F10D.12 Caenorhabditis elegans larval arrest wormbase
CELE_Y45F10D.12 Caenorhabditis elegans slow growth wormbase
CELE_Y45F10D.12 Caenorhabditis elegans sterile wormbase
YOL120C Saccharomyces cerevisiae inviable yeastgenome
TGME49_300000 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.504147.20 (Trypanosoma cruzi), 60S ribosomal protein L18, putative
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