Detailed view for TcCLB.439125.10

Basic information

TDR Targets ID: 30715
Trypanosoma cruzi, Peptide chain release factor 1, mitochondrial, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.9688 | Length (AA): 270 | MW (Da): 29044 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00472   RF-1 domain
PF03462   PCRF domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0003747   translation release factor activity  
GO:0006415   translational termination  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 257 2b9m (Y) 123 362 30.00 0 1 0.92 2.03
3 258 1rq0 (A) 97 333 39.00 0 1 1.23 0.49
3 258 1rq0 (A) 97 333 39.00 0 1 1.25455 0.21
107 191 2jva (A) 1 107 25.00 0 0.86 0.344715 -0.17
108 194 2jy9 (A) 1 112 22.00 0.0097 0.5 0.285622 0.14

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126947)

Species Accession Gene Product
Arabidopsis thaliana AT2G47020   peptide chain release factor 1
Arabidopsis thaliana AT3G62910   protein CHLOROPLAST RIBOSOME RELEASE FACTOR 1
Babesia bovis BBOV_IV006160   peptide chain release factor 1, putative
Brugia malayi Bm1_54480   Peptidyl-tRNA hydrolase domain containing protein
Candida albicans CaO19.1303   Mitochondrial peptide chain release factor
Candida albicans CaO19.8883   Mitochondrial peptide chain release factor
Caenorhabditis elegans CELE_W03F8.3   Protein W03F8.3
Chlamydia trachomatis CT_023   peptide chain release factor
Dictyostelium discoideum DDB_G0283175   class I peptide chain release factor
Drosophila melanogaster Dmel_CG5705   CG5705 gene product from transcript CG5705-RA
Escherichia coli b1211   peptide chain release factor RF-1
Echinococcus granulosus EgrG_000572700   peptide chain release factor 1
Echinococcus multilocularis EmuJ_000572700   peptide chain release factor 1
Echinococcus multilocularis EmuJ_001188000   inner nuclear membrane protein man1
Homo sapiens ENSG00000112031   mitochondrial translational release factor 1-like
Homo sapiens ENSG00000120662   mitochondrial translational release factor 1
Leishmania braziliensis LbrM.25.1310   peptide chain release factor-like protein
Leishmania donovani LdBPK_251510.1   Peptide chain release factor 1, mitochondrial, putative
Leishmania infantum LinJ.25.1510   peptide chain release factor-like protein
Leishmania major LmjF.25.1450   peptide chain release factor-like protein
Leishmania mexicana LmxM.25.1450   peptide chain release factor-like protein
Loa Loa (eye worm) LOAG_08061   peptidyl-tRNA hydrolase domain-containing protein
Mycobacterium leprae ML1134   PROBABLE PEPTIDE CHAIN RELEASE FACTOR 1 PRFA (RF-1)
Mus musculus ENSMUSG00000019774   mitochondrial translational release factor 1-like
Mus musculus ENSMUSG00000022022   mitochondrial translational release factor 1
Mycobacterium tuberculosis Rv1299   Probable peptide chain release factor 1 PrfA (RF-1)
Mycobacterium ulcerans MUL_3965   peptide chain release factor 1
Neospora caninum NCLIV_057350   hypothetical protein
Neospora caninum NCLIV_002600   peptide chain release factor 1, putative
Oryza sativa 4338281   Os05g0277300
Oryza sativa 4351050   Os11g0657100
Onchocerca volvulus OVOC4071   Putative peptide chain release factor 1, mitochondrial
Plasmodium berghei PBANKA_1016100   peptide chain release factor 1, putative
Plasmodium berghei PBANKA_0833200   peptide chain release factor 1, putative
Plasmodium falciparum PF3D7_1428600   peptide chain release factor 1
Plasmodium falciparum PF3D7_0932400   peptide chain release factor 1
Plasmodium knowlesi PKNH_1330100   peptide chain release factor 1, putative
Plasmodium knowlesi PKNH_0731100   peptide chain release factor 1, putative
Plasmodium vivax PVX_085115   peptide chain release factor 1, putative
Plasmodium vivax PVX_087085   peptide release factor, putative
Plasmodium yoelii PY03620   peptide chain release factor 1
Saccharomyces cerevisiae YGL143C   Mrf1p
Schistosoma japonicum Sjp_0213450   Inner nuclear membrane protein Man1, putative
Schistosoma japonicum Sjp_0213460   ko:K02835 peptide chain release factor RF-1, putative
Schistosoma mansoni Smp_047560   peptide chain release factor
Schmidtea mediterranea mk4.000067.08  
Schmidtea mediterranea mk4.002527.03   Probable peptide chain release factor 1, mitochondrial
Schmidtea mediterranea mk4.002527.02  
Schmidtea mediterranea mk4.003200.08  
Trypanosoma brucei gambiense Tbg972.3.780   peptide chain release factor 1, putative
Trypanosoma brucei Tb927.3.1070   Peptide chain release factor 1, mitochondrial, putative
Trypanosoma congolense TcIL3000_0_51810   Peptide chain release factor 1 like, mitochondrial, putative
Trypanosoma congolense TcIL3000_3_290   Peptide chain release factor 1, mitochondrial, putative
Trypanosoma cruzi TcCLB.509455.120   Peptide chain release factor 1, mitochondrial, putative
Trypanosoma cruzi TcCLB.439125.10   Peptide chain release factor 1, mitochondrial, putative
Toxoplasma gondii TGME49_314270   peptidyl-tRNA hydrolase domain-containing protein
Toxoplasma gondii TGME49_207630   peptidyl-tRNA hydrolase domain-containing protein
Treponema pallidum TP0051   peptide chain release factor 1
Theileria parva TP01_0422   peptide chain release factor 1, putative
Theileria parva TP03_0303   hypothetical protein
Theileria parva TP03_0302   peptide chain release factor 1, putative
Wolbachia endosymbiont of Brugia malayi Wbm0358   peptide chain release factor 1

Essentiality

TcCLB.439125.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1321 Mycobacterium tuberculosis essential nmpdr
Tb927.3.1070 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.1070 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.1070 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.1070 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b1211 Escherichia coli essential goodall
PBANKA_1016100 Plasmodium berghei Dispensable plasmo
TGME49_314270 Toxoplasma gondii Probably essential sidik
TGME49_207630 Toxoplasma gondii Probably essential sidik
TGME49_314270 Toxoplasma gondii Essentiality uncertain sidik
TGME49_207630 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.439125.10 (Trypanosoma cruzi), Peptide chain release factor 1, mitochondrial, putative
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