Detailed view for PF3D7_0105300

Basic information

TDR Targets ID: 3174
Plasmodium falciparum, cyclase-associated protein
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 4.8666 | Length (AA): 162 | MW (Da): 18528 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08603   Adenylate cyclase associated (CAP) C terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0007015   actin filament organization  
GO:0005488   binding  
GO:0003779   actin binding  
GO:0007010   cytoskeleton organization and biogenesis  
GO:0000902   cell morphogenesis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 159 1k8f (A) 319 473 37.00 0 1 1.45 -1.14
5 157 2b0r (A) 43 198 37.00 0 1 1.49 -1.21
2 157 2b0r (A) 40 198 37.00 0 1 1.51556 -1.24
4 159 1k8f (A) 319 473 37.00 0 1 1.54656 -1.25

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 2B0R:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont, late schizont, Oocyst, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, gametocyte. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 8 hs, Ring, Sporozoite. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 16 hs, late trophozoite. Otto TD PlasmoDB
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_127770)

Species Accession Gene Product
Arabidopsis thaliana AT4G34490   cyclase associated protein 1
Babesia bovis BBOV_I003280   conserved hypothetical protein
Brugia malayi Bm1_01905   CAP protein
Candida albicans CaO19.8135   adenylate cyclase-associated protein involved in regulation of cyclic AMP levels and filamentous growth
Candida albicans CaO19.505   adenylate cyclase-associated protein involved in regulation of cyclic AMP levels and filamentous growth
Caenorhabditis elegans CELE_F41G4.2   Protein CAS-1, isoform B
Cryptosporidium hominis Chro.50350   LD24380p
Cryptosporidium parvum cgd5_440   protein with 2 CAP (CARP) domains, possible adenyl cyclase-associated protein
Dictyostelium discoideum DDB_G0288769   cyclase associated protein
Drosophila melanogaster Dmel_CG33979   capulet
Echinococcus granulosus EgrG_000897100   adenylyl cyclase associated protein 1
Entamoeba histolytica EHI_136150   adenylyl cyclase-associated protein, putative
Entamoeba histolytica EHI_081430   adenylylcyclase-associated protein, putative
Echinococcus multilocularis EmuJ_000897100   adenylyl cyclase associated protein 1
Homo sapiens ENSG00000112186   CAP, adenylate cyclase-associated protein, 2 (yeast)
Homo sapiens ENSG00000131236   CAP, adenylate cyclase-associated protein 1 (yeast)
Leishmania braziliensis LbrM.35.5860   G-actin binding protein, putative,CAP/Srv2p, putative
Leishmania donovani LdBPK_365830.1   adenylyl cyclase-associated protein, putative
Leishmania infantum LinJ.36.5830   G-actin binding protein, putative,CAP/Srv2p, putative
Leishmania major LmjF.36.5590   G-actin binding protein, putative,CAP/Srv2p, putative
Loa Loa (eye worm) LOAG_15179   hypothetical protein
Loa Loa (eye worm) LOAG_07646   hypothetical protein
Mus musculus ENSMUSG00000028656   CAP, adenylate cyclase-associated protein 1 (yeast)
Mus musculus ENSMUSG00000021373   CAP, adenylate cyclase-associated protein, 2 (yeast)
Neospora caninum NCLIV_054140   adenylyl cyclase associated protein, putative
Oryza sativa 4345716   Os08g0447900
Oryza sativa 4333945   Os03g0722600
Plasmodium berghei PBANKA_0208000   cyclase-associated protein
Plasmodium falciparum PF3D7_0105300   cyclase-associated protein
Plasmodium knowlesi PKNH_0208400   cyclase-associated protein, putative
Plasmodium vivax PVX_081498   hypothetical protein, conserved
Plasmodium vivax PVX_081502   hypothetical protein, conserved
Saccharomyces cerevisiae YNL138W   Srv2p
Schistosoma japonicum Sjp_0203560   Adenylyl cyclase-associated protein 1, putative
Schistosoma mansoni Smp_014010   adenylyl cyclase-associated protein
Schmidtea mediterranea mk4.007218.01   Adenylyl cyclase-associated protein
Schmidtea mediterranea mk4.015808.00   Adenylyl cyclase-associated protein
Trypanosoma brucei gambiense Tbg972.10.11320   G-actin binding protein, putative,CAP/Srv2p, putative
Trypanosoma brucei Tb927.10.9250   adenylyl cyclase-associated protein, putative
Trypanosoma congolense TcIL3000_10_8020   adenylyl cyclase-associated protein, putative
Trypanosoma cruzi TcCLB.509799.30   adenylyl cyclase-associated protein, putative
Trypanosoma cruzi TcCLB.504137.80   adenylyl cyclase-associated protein, putative
Toxoplasma gondii TGME49_310030   cyclase-associated protein, putative
Theileria parva TP03_0176   hypothetical protein, conserved
Trichomonas vaginalis TVAG_407250   adenylyl cyclase-associated protein, putative

Essentiality

PF3D7_0105300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.9250 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.9250 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.9250 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.9250 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
YNL138W Saccharomyces cerevisiae inviable yeastgenome
TGME49_310030 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0105300 (Plasmodium falciparum), cyclase-associated protein
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