Detailed view for PF3D7_0217100

Basic information

TDR Targets ID: 3433
Plasmodium falciparum, ATP synthase F1, alpha subunit
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.6346 | Length (AA): 551 | MW (Da): 61770 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00006   ATP synthase alpha/beta family, nucleotide-binding domain
PF00306   ATP synthase alpha/beta chain, C terminal domain
PF02874   ATP synthase alpha/beta family, beta-barrel domain

Gene Ontology

Mouse over links to read term descriptions.
GO:1902600   GO:proton transmembrane transport  

GO:0046933   hydrogen ion transporting ATP synthase activity, rotational mechanism  
GO:0045261   proton-transporting ATP synthase complex, catalytic core F(1)  
GO:0032559   adenyl ribonucleotide binding  
GO:0016469   proton-transporting two-sector ATPase complex  
GO:0005739   mitochondrion  
GO:0005524   ATP binding  
GO:0046034   ATP metabolic process  
GO:0015992   proton transport  
GO:0015986   ATP synthesis coupled proton transport  

Metabolic Pathways

Oxidative phosphorylation (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
54 547 1sky (B) 21 498 56.00 0 1 1.64 -1.31
57 548 2ck3 (A) 24 509 63.00 0 1 1.73 -1.79
36 547 5dn6 (A) 3 510 58.00 0 1 1.65772 -0.78
42 548 1mab (A) 10 509 62.00 0 1 1.63775 -0.22
50 548 2r9v (A) 17 502 57.00 0 1 1.63813 -1.02
60 547 1fx0 (A) 28 501 53.00 0 1 1.50526 -0.34

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 32 hs, gametocyte, sporozoite, early schizont, early trophozoite, late schizont, late trophozoite, Oocyst, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, merozoite, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, late ring, Ring. Otto TD PlasmoDB Zanghi G
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_127165)

Species Accession Gene Product
Arabidopsis thaliana ArthCp007   ATP synthase CF1 alpha chain
Arabidopsis thaliana AT2G07698   ATPase, F1 complex, alpha subunit protein
Arabidopsis thaliana ArthMp100   ATPase subunit 1
Babesia bovis BBOV_III002130   ATP synthase F1, alpha subunit family protein
Brugia malayi Bm1_47570   ATP synthase alpha chain, mitochondrial precursor, putative
Candida albicans CaO19.14144   C terminus of mitochondrial F1F0 H+ATPase
Candida albicans CaO19.6854   C terminus of mitochondrial F1F0 H+ATPase
Caenorhabditis elegans CELE_H28O16.1   Protein H28O16.1, isoform B
Cryptosporidium hominis Chro.60082   ATP synthase alpha chain
Cryptosporidium parvum cgd6_610   ATP synthase alpha chain
Dictyostelium discoideum DidioMp11   ATPase subunit 1
Drosophila melanogaster Dmel_CG3612   bellwether
Escherichia coli b3734   F1 sector of membrane-bound ATP synthase, alpha subunit
Echinococcus granulosus EgrG_000720500   ATP synthase subunit alpha mitochondrial
Echinococcus multilocularis EmuJ_000720500   ATP synthase subunit alpha, mitochondrial
Homo sapiens ENSG00000152234   ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Leishmania braziliensis LbrM.05.0520   ATPase alpha subunit
Leishmania donovani LdBPK_050510.1   ATP synthase F1, alpha subunit, putative
Leishmania infantum LinJ.05.0500   ATPase alpha subunit
Leishmania infantum LinJ.05.0510   ATPase alpha subunit
Leishmania major LmjF.05.0500   ATPase alpha subunit
Leishmania major LmjF.05.0510   ATPase alpha subunit
Leishmania mexicana LmxM.05.0510   ATPase alpha subunit, putative
Leishmania mexicana LmxM.05.0500   ATPase alpha subunit, putative
Leishmania mexicana LmxM.05.0510b  
Mycobacterium leprae ML1143   PROBABLE ATP SYNTHASE ALPHA CHAIN ATPA
Mus musculus ENSMUSG00000025428   ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1
Mycobacterium tuberculosis Rv1308   Probable ATP synthase alpha chain AtpA
Mycobacterium ulcerans MUL_3956   F0F1 ATP synthase subunit alpha
Neospora caninum NCLIV_020840   hypothetical protein
Oryza sativa 3131390   ATP synthase CF1 alpha chain
Oryza sativa 6450183   ATP synthase F0 subunit 1
Onchocerca volvulus OVOC1523   ATP synthase subunit alpha, mitochondrial homolog
Plasmodium berghei PBANKA_0313800   ATP synthase F1, alpha subunit, putative
Plasmodium falciparum PF3D7_0217100   ATP synthase F1, alpha subunit
Plasmodium knowlesi PKNH_0403800   ATP synthase F1, alpha subunit, putative
Plasmodium vivax PVX_002685   ATP synthase alpha chain, putative
Plasmodium yoelii PY05971   ATP synthase F1, alpha subunit
Saccharomyces cerevisiae YBL099W   F1F0 ATP synthase subunit alpha
Schistosoma japonicum Sjp_0089940   ko:K02132 F-type H+-transporting ATPase alpha chain, putative
Schistosoma mansoni Smp_002880.2   ATP synthase alpha subunit mitochondrial
Schmidtea mediterranea mk4.000367.04  
Schmidtea mediterranea mk4.010027.01   ATP synthase subunit alpha, mitochondrial
Schmidtea mediterranea mk4.000367.03   ATP synthase subunit alpha, mitochondrial
Trypanosoma brucei gambiense Tbg972.7.8720   ATP synthase alpha chain, mitochondrial precursor, putative
Trypanosoma brucei Tb927.7.7430   ATP synthase F1, alpha subunit
Trypanosoma brucei Tb927.7.7420   ATP synthase F1, alpha subunit
Trypanosoma congolense TcIL3000_7_6050   ATP synthase F1, alpha subunit, putative
Theileria parva TP03_0569   ATP synthase F1, alpha chain, putative
Wolbachia endosymbiont of Brugia malayi Wbm0314   ATP synthase F0F1 subunit alpha

Essentiality

PF3D7_0217100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1330 Mycobacterium tuberculosis essential nmpdr
Tb927.7.7420 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.7420 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.7420 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.7420 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3734 Escherichia coli non-essential goodall
CELE_H28O16.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_H28O16.1 Caenorhabditis elegans larval arrest wormbase
CELE_H28O16.1 Caenorhabditis elegans slow growth wormbase
CELE_H28O16.1 Caenorhabditis elegans sterile wormbase
CELE_H28O16.1 Caenorhabditis elegans terminal arrest variable wormbase
PBANKA_0313800 Plasmodium berghei Slow plasmo
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mycobacterium tuberculosis Probable ATP synthase alpha chain AtpA Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0044 0.6052 0.6505
0.0085 0.3625 0.2671
0.0332 0.6996 0.6996
0.0087 0.2689 0.2689
0.008 0.3271 0.2654
0.0085 0.3625 0.2671
0.0087 0.2699 0.2699
0.0079 0.3283 0.2672
0.0088 0.2905 0.2113
0.0336 0.764 0.7611
0.0073 0.3229 0.2593
0.0213 0.7157 0.6996
0.0079 0.3283 0.2672
0.0077 0.2654 0.2654
0.0056 0.4835 0.4241
0.0092 0.3006 0.3006

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

11 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_0217100 (Plasmodium falciparum), ATP synthase F1, alpha subunit
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