Detailed view for TcCLB.447705.9

Basic information

TDR Targets ID: 35701
Trypanosoma cruzi, EMG1/NEP1 methyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.1515 | Length (AA): 216 | MW (Da): 24762 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03587   EMG1/NEP1 methyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005634   nucleus  
GO:0008168   methyltransferase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
11 205 3oii (A) 28 237 32.00 0 1 1.25378 -0.37
16 215 5fai (A) 36 230 39.00 0 1 1.36293 -0.03

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127748)

Species Accession Gene Product
Arabidopsis thaliana AT3G57000   nucleolar essential protein-related protein
Babesia bovis BBOV_I002890   suppressor Mra1 family domain containing protein
Brugia malayi Bm1_42740   Probable ribosome biogenesis protein NEP1
Candida albicans CaO19.8282   conserved Nucleolar Essential Protein involved in rRNA processing
Candida albicans CaO19.665   conserved Nucleolar Essential Protein involved in rRNA processing
Caenorhabditis elegans CELE_Y39A1A.14   Protein Y39A1A.14
Cryptosporidium hominis Chro.40319   ribosome biogenesis protein nep1
Cryptosporidium parvum cgd4_2840   Mra1/NEP1 like protein, involved in pre-rRNA processing, adjacent genes putative paralogs
Dictyostelium discoideum DDB_G0272732   hypothetical protein
Drosophila melanogaster Dmel_CG3527   CG3527 gene product from transcript CG3527-RA
Echinococcus granulosus EgrG_000174900   ribosomal RNA small subunit methyltransferase
Entamoeba histolytica EHI_044610   ribosome biogenesis protein NEP1, putative
Echinococcus multilocularis EmuJ_000174900   ribosomal RNA small subunit methyltransferase
Giardia lamblia GL50803_5786   Ribosome biogenesis protein NEP1, putative
Homo sapiens 10436   EMG1 N1-specific pseudouridine methyltransferase
Leishmania braziliensis LbrM.16.1450   hypothetical protein, conserved
Leishmania donovani LdBPK_161470.1   EMG1/NEP1 methyltransferase, putative
Leishmania infantum LinJ.16.1470   hypothetical protein, conserved
Leishmania major LmjF.16.1390   hypothetical protein, conserved
Leishmania mexicana LmxM.16.1390   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_03665   C2f protein
Mus musculus ENSMUSG00000004268   EMG1 nucleolar protein homolog (S. cerevisiae)
Neospora caninum NCLIV_057180   Suppressor Mra1 superfamily, related
Oryza sativa 4329057   Os02g0290400
Plasmodium berghei PBANKA_0709300   ribosomal RNA small subunit methyltransferase NEP1, putative
Plasmodium falciparum PF3D7_0821500   ribosomal RNA small subunit methyltransferase NEP1, putative
Plasmodium knowlesi PKNH_1315500   ribosomal RNA small subunit methyltransferase NEP1, putative
Plasmodium vivax PVX_089290   ribosomal RNA small subunit methyltransferase NEP1, putative
Saccharomyces cerevisiae YLR186W   Emg1p
Schistosoma japonicum Sjp_0066750   Probable ribosome biogenesis protein NEP1, putative
Schistosoma mansoni Smp_002010   nep1
Schmidtea mediterranea mk4.004989.00   Putative nep1
Schmidtea mediterranea mk4.003823.06   Putative nep1
Trypanosoma brucei gambiense Tbg972.8.4840   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.5040   EMG1/NEP1 methyltransferase, putative
Trypanosoma congolense TcIL3000_8_4790   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.447705.9   EMG1/NEP1 methyltransferase, putative
Trypanosoma cruzi TcCLB.508961.20   EMG1/NEP1 methyltransferase, putative
Trypanosoma cruzi TcCLB.508839.40   EMG1/NEP1 methyltransferase, putative
Toxoplasma gondii TGME49_313980   ribosome biogenesis protein nep1, putative
Theileria parva TP01_0556   hypothetical protein
Trichomonas vaginalis TVAG_312150   conserved hypothetical protein

Essentiality

TcCLB.447705.9 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.5040 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.5040 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.5040 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.5040 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y39A1A.14 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y39A1A.14 Caenorhabditis elegans larval arrest wormbase
YLR186W Saccharomyces cerevisiae inviable yeastgenome
TGME49_313980 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TcCLB.447705.9 (Trypanosoma cruzi), EMG1/NEP1 methyltransferase, putative
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