Detailed view for PF3D7_0422000

Basic information

TDR Targets ID: 3990
Plasmodium falciparum, steroid dehydrogenase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.9434 | Length (AA): 321 | MW (Da): 37215 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00106   short chain dehydrogenase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  
GO:0006633   fatty acid biosynthetic process  

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
44 328 1yxm (A) 7 300 17.00 0 1 1.08 -1.14
57 246 1xkq (A) 8 197 24.00 0.0000000002 1 1.07 -1.96
57 319 1jtv (A) 4 266 20.00 0.000000017 1 1.19 -1.42
35 308 2rhc (B) 0 254 18.00 0 1 0.885383 -0.15
43 290 2ae2 (A) 4 249 20.00 0 1 1.07399 -0.71
48 239 2z1n (A) 7 192 22.00 0.067 0.95 0.887231 -0.55
49 102 1jay (A) 2 54 19.00 0.3 0.61 0.515624 -1.36
244 288 1tnr (A) 60 104 42.00 0.87 0.04 0.226287 2.87

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile gametocyte, sporozoite, Oocyst, Ring, Female Gametocyte. PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 48 hs, early ring, early trophozoite, late ring, late trophozoite, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 40 hs, early schizont, late schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, Male Gametocyte. Otto TD Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_126812)

Species Accession Gene Product
Arabidopsis thaliana AT1G67730   beta-ketoacyl reductase 1
Arabidopsis thaliana AT1G24470   beta-ketoacyl reductase 2
Brugia malayi Bm1_44630   oxidoreductase, short chain dehydrogenase/reductase family protein
Brugia malayi Bm1_45995   oxidoreductase, short chain dehydrogenase/reductase family protein
Candida albicans CaO19.3859   similar to human 17beta-hydroxysteroid dehydrogenase
Candida albicans CaO19.11340   similar to human 17beta-hydroxysteroid dehydrogenase
Caenorhabditis elegans CELE_F25G6.5   Protein STDH-4
Caenorhabditis elegans CELE_C56G2.6   Protein LET-767, isoform A
Cryptosporidium hominis Chro.30277   F12A21.14
Cryptosporidium parvum cgd3_2370   steroid dehydrogenase kik-i, putative
Dictyostelium discoideum DDB_G0275049   short-chain dehydrogenase/reductase family protein
Drosophila melanogaster Dmel_CG31810   CG31810 gene product from transcript CG31810-RA
Drosophila melanogaster Dmel_CG31809   CG31809 gene product from transcript CG31809-RB
Drosophila melanogaster Dmel_CG1444   CG1444 gene product from transcript CG1444-RA
Drosophila melanogaster Dmel_CG13284   CG13284 gene product from transcript CG13284-RE
Echinococcus granulosus EgrG_000624600   steroid dehydrogenase
Echinococcus granulosus EgrG_000953000   steroid dehydrogenase
Entamoeba histolytica EHI_163540   estradiol 17-beta-dehydrogenase, putative
Entamoeba histolytica EHI_165070   estradiol 17-beta-dehydrogenase, putative
Echinococcus multilocularis EmuJ_000284400   estradiol 17 beta dehydrogenase 12
Echinococcus multilocularis EmuJ_000953000   steroid dehydrogenase
Echinococcus multilocularis EmuJ_000284700   steroid dehydrogenase
Echinococcus multilocularis EmuJ_000624600   steroid dehydrogenase
Giardia lamblia GL50803_14626   Oxidoreductase, short chain dehydrogenase/reductase family
Homo sapiens ENSG00000149084   hydroxysteroid (17-beta) dehydrogenase 12
Homo sapiens ENSG00000103160   hydroxysteroid dehydrogenase like 1
Leishmania braziliensis LbrM.20.0090   hypothetical protein, conserved
Leishmania donovani LdBPK_340010.1   short chain dehydrogenase, putative
Leishmania infantum LinJ.34.0010   hypothetical protein, conserved
Leishmania major LmjF.34.0010   hypothetical protein, conserved
Leishmania mexicana LmxM.33.0010   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_00557   hypothetical protein
Loa Loa (eye worm) LOAG_04043   hypothetical protein
Mycobacterium leprae ML0429c   Probable short-chain type dehydrogenase/reductase
Mus musculus ENSMUSG00000034189   hydroxysteroid dehydrogenase like 1
Mus musculus ENSMUSG00000027195   hydroxysteroid (17-beta) dehydrogenase 12
Mycobacterium tuberculosis Rv1544   Possible ketoacyl reductase
Mycobacterium ulcerans MUL_1543   ketoacyl reductase
Mycobacterium ulcerans MUL_4860   ketoacyl reductase
Mycobacterium ulcerans MUL_0124   ketoacyl reductase
Neospora caninum NCLIV_035250   GK18150, related
Oryza sativa 4329873   Os02g0597900
Oryza sativa 4336202   Os04g0483500
Plasmodium berghei PBANKA_0522400   steroid dehydrogenase, putative
Plasmodium falciparum PF3D7_0422000   steroid dehydrogenase, putative
Plasmodium knowlesi PKNH_0513800   steroid dehydrogenase, putative
Plasmodium vivax PVX_090140   steroid dehydrogenase, putative
Plasmodium yoelii PY05567   short chain dehydrogenase, putative
Saccharomyces cerevisiae YBR159W   ketoreductase
Schistosoma japonicum Sjp_0217990   Estradiol 17-beta-dehydrogenase 12, putative
Schistosoma japonicum Sjp_0123070   IPR002347,Glucose/ribitol dehydrogenase,domain-containing
Schistosoma japonicum Sjp_0303510   Estradiol 17-beta-dehydrogenase 12, putative
Schistosoma japonicum Sjp_0058300   ko:K00100 HSD17B12; hydroxysteroid (17-beta) dehydrogenase 12 [EC:1.1.1.-], putative
Schistosoma japonicum Sjp_0211990   ko:K00044 estradiol 17beta-dehydrogenase [EC1.1.1.62], putative
Schistosoma mansoni Smp_168550   steroid dehydrogenase
Schistosoma mansoni Smp_117920   steroid dehydrogenase
Schistosoma mansoni Smp_084570   steroid dehydrogenase
Schistosoma mansoni Smp_009430   steroid dehydrogenase
Schistosoma mansoni Smp_115700   steroid dehydrogenase
Schistosoma mansoni Smp_120510   steroid dehydrogenase
Schistosoma mansoni Smp_168560   steroid dehydrogenase
Schistosoma mansoni Smp_178940   steroid dehydrogenase
Schistosoma mansoni Smp_102190   steroid dehydrogenase
Schmidtea mediterranea mk4.000773.03   Estradiol 17-beta-dehydrogenase 12
Schmidtea mediterranea mk4.000172.18  
Schmidtea mediterranea mk4.002200.00  
Schmidtea mediterranea mk4.003395.00  
Schmidtea mediterranea mk4.003395.01   Testosterone 17-beta-dehydrogenase 3
Schmidtea mediterranea mk4.007959.01  
Trypanosoma brucei gambiense Tbg972.5.1680   T. brucei spp.-specific protein
Trypanosoma brucei Tb927.5.1210   short-chain dehydrogenase, putative
Trypanosoma congolense TcIL3000_5_1120   short-chain dehydrogenase, putative
Trypanosoma cruzi TcCLB.509767.170   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506219.40   hypothetical protein, conserved
Toxoplasma gondii TGME49_271888   3-ketoacyl-CoA reductase, putative

Essentiality

PF3D7_0422000 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1572 Mycobacterium tuberculosis non-essential nmpdr
Tb927.5.1210 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.1210 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.1210 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.5.1210 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C56G2.6 Caenorhabditis elegans adult lethal wormbase
CELE_C56G2.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_C56G2.6 Caenorhabditis elegans larval arrest wormbase
CELE_C56G2.6 Caenorhabditis elegans slow growth wormbase
CELE_C56G2.6 Caenorhabditis elegans sterile wormbase
PBANKA_0522400 Plasmodium berghei Essential plasmo
TGME49_271888 Toxoplasma gondii Probably essential sidik
TGME49_271888 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Macaca mulatta Hydroxysteroid 11-beta dehydrogenase 1 140 aa 27.8% 151 aa Compounds References
Rattus norvegicus Testosterone 17-beta-dehydrogenase 3 306 aa 29.0% 293 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0021 0.3321 0.5
0.0076 1 0.5
0.0026 0.5 0.5
0.004 0.5 0.5
0.0087 0.5 0.5
0.0064 0.3508 0.5
0.0009 0.5 0.5
0.0009 0.5 0.5
0.0009 0.5 0.5
0.0408 0.5144 1
0.0408 0.5144 1
0.0073 0.4546 1
0.0171 0.4063 0.5
0.0045 1 1
0.0039 1 0.5
0.0009 0.5 0.5
0.0032 1 1
0.0058 1 1
0.0004 0.5 0.5
0.0068 1 1
0.0106 1 1
0.0004 0.5 0.5
0.0027 0.5 0.5
0.0004 0.5 0.5
0.0408 0.5144 1
0.007 1 0.5
0.0053 1 1
0.0093 0.5 0.5
0.0114 1 0.5
0.008 0.5 0.5
0.007 1 0.5
0.0196 0.8679 1
0.0113 1 1
0.0088 1 0.5
0.0005 0.5 0.5
0.0106 0.5 0.5
0.0009 1 0.5
0.0106 1 0.5
0.0123 0.5 0.5
0.0196 0.8679 1
0.0004 0.5 0.5
0.0004 0.5 0.5
0.0106 0.2872 1
0.0004 0.5 0.5
0.0019 0.4322 1
0.0068 1 1
0.0071 0.3141 0.5
0.0157 0.5 0.5
0.0067 0.3468 0.5
0.0066 0.3586 0.5
0.011 0.3568 1
0.0005 0.5 0.5
0.0068 0.2585 0.5
0.0053 1 1
0.0084 0.4283 0.2703
0.0013 0.5 0.5
0.0089 0.5 0.5
0.0085 1 1
0.007 0.2916 1
0.0058 0.3931 1
0.016 0.577 1
0.0222 0.5 0.5
0.0073 0.3811 1
0.0009 0.5 0.5
0.0012 0.5 0.5
0.0056 1 0.5
0.0056 0.875 0.5
0.0082 1 0.5
0.0057 0.284 0.6352
0.0089 0.5 0.5
0.0131 0.5 0.5
0.0402 1 1
0.0016 0.5 0.5
0.0013 1 0.5
0.0196 0.8679 1
0.0119 0.2746 1
0.0009 0.7374 0.5
0.0046 0.3082 0
0.0021 0.3321 0.5
0.0171 0.4063 0.5
0.0004 0.5 0.5
0.0073 0.3811 1
0.0009 0.5 0.5
0.016 0.577 1
0.0162 1 0.5
0.0108 0.5 0.5
0.0009 0.5 0.5
0.0009 0.5 0.5
0.0114 1 0.5
0.0035 1 0.5
0.0097 1 1
0.0021 0.5 0.5
0.0073 0.2873 0.7466
0.0027 0.5 0.5
0.0132 0.3658 0.5
0.0073 0.3141 1
0.0088 0.6544 0.5
0.0102 0.7451 0.5
0.007 0.2916 1
0.0097 0.4304 0.5
0.007 0.2916 1
0.007 1 0.5
0.0165 1 1
0.0218 0.5 0.5
0.0012 0.5 0.5
0.0118 1 0.5

Assayability

Assay information

  • Assay for Hydroxysteroid Dehyrogenase (1.1.1.- ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Plasmodium falciparum ( 4 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    PF3D7_0422000 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Plasmodium falciparum ( 4 )

Bibliographic References

5 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_0422000 (Plasmodium falciparum), steroid dehydrogenase, putative
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