Detailed view for TcCLB.503745.30

Basic information

TDR Targets ID: 40973
Trypanosoma cruzi, ascorbate peroxidase
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.6053 | Length (AA): 328 | MW (Da): 36501 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00141   Peroxidase

Gene Ontology

Mouse over links to read term descriptions.
GO:0020037   heme binding  
GO:0004601   peroxidase activity  
GO:0055114   oxidation reduction  
GO:0006979   response to oxidative stress  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
40 327 2cca (A) 47 435 32.00 0 1 0.92 -0.55
40 325 2eut (A) 4 282 40.00 0 1 1.38 -1.06
11 321 5jhx (A) 439 783 21.00 0 1 1.07967 -0.02
59 325 5al9 (A) 34 300 67.00 0 1 1.75582 -1.85

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_128717)

Species Accession Gene Product
Arabidopsis thaliana AT4G35000   L-ascorbate peroxidase
Arabidopsis thaliana AT4G35970   L-ascorbate peroxidase
Arabidopsis thaliana AT3G09640   L-ascorbate peroxidase 2
Candida albicans CaO19.584   CCP1-like orf
Candida albicans CaO19.7868   Cytochrome-c peroxidase
Candida albicans CaO19.8216   CCP1-like orf
Candida albicans CaO19.238   Cytochrome-c peroxidase
Escherichia coli b3942   catalase-peroxidase HPI, heme b-containing
Leishmania braziliensis LbrM.20.0150   ascorbate-dependent peroxidase, putative
Leishmania donovani LdBPK_340070.1   ascorbate peroxidase, putative
Leishmania infantum LinJ.34.0070   ascorbate-dependent peroxidase, putative
Leishmania major LmjF.34.0070   ascorbate peroxidase
Leishmania mexicana LmxM.33.0070   ascorbate-dependent peroxidase, putative
Mycobacterium tuberculosis Rv1908c   Catalase-peroxidase-peroxynitritase T KatG
Mycobacterium ulcerans MUL_2190   catalase-peroxidase-peroxynitritase T KatG
Oryza sativa 9269342   Os09g0538600
Oryza sativa 4346247   Os08g0549100
Oryza sativa 4335202   Os04g0223300
Saccharomyces cerevisiae YKR066C   Ccp1p
Trypanosoma cruzi TcCLB.503745.30   ascorbate peroxidase
Trypanosoma cruzi TcCLB.506193.60   ascorbate peroxidase, putative

Essentiality

TcCLB.503745.30 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b3942 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for NADH Peroxidase pH 6 (1.11.1.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma cruzi ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    TcCLB.503745.30 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Trypanosoma cruzi ( 1 )

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.503745.30 (Trypanosoma cruzi), ascorbate peroxidase
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