Detailed view for TcCLB.503489.10

Basic information

TDR Targets ID: 41366
Trypanosoma cruzi, metallo-peptidase, Clan MC, Family M14, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.8638 | Length (AA): 722 | MW (Da): 79647 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00246   Zinc carboxypeptidase

Gene Ontology

Mouse over links to read term descriptions.
GO:0008270   zinc ion binding  
GO:0004181   metallocarboxypeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
47 398 4b6z (A) 20 320 22.00 0 1 0.518235 0.67
68 387 4b6z (A) 41 307 31.00 0 0.98 0.476913 0.95
137 411 3l2n (A) 91 331 25.00 0 1 0.483086 0.27
158 354 1m4l (A) 7 204 14.00 0 0.69 0.0780532 -0.17
243 385 3l2n (A) 161 298 33.00 0 0.63 0.476761 0.55
648 709 5hzp (C) 58 119 13.00 0 0 0.403073 -1.98
663 702 1qbk (B) 321 368 45.00 0.66 0.22 0.473102 -0.25

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127925)

Species Accession Gene Product
Brugia malayi Bm1_11405   Zinc carboxypeptidase family protein
Caenorhabditis elegans CELE_F56H1.5   Protein CCPP-1
Drosophila melanogaster Dmel_CG44533   Drosophila Nna1 ortholog
Echinococcus granulosus EgrG_000352300   cytosolic carboxypeptidase 1
Echinococcus granulosus EgrG_000261800   cytosolic carboxypeptidase 2
Echinococcus multilocularis EmuJ_000352300  
Homo sapiens ENSG00000166748   ATP/GTP binding protein-like 1
Homo sapiens ENSG00000165923   ATP/GTP binding protein-like 2
Homo sapiens ENSG00000146856   ATP/GTP binding protein-like 3
Homo sapiens ENSG00000135049   ATP/GTP binding protein 1
Leishmania braziliensis LbrM.33.0210   zinc carboxypeptidase, putative,metallo-peptidase, Clan MC, Family M14
Leishmania donovani LdBPK_330210.1   metallo-peptidase, Clan MC, Family M14, putative
Leishmania infantum LinJ.33.0210   zinc carboxypeptidase, putative,metallo-peptidase, Clan MC, Family M14
Leishmania major LmjF.33.0200   zinc carboxypeptidase, putative,metallo-peptidase, Clan MC, Family M14
Leishmania mexicana LmxM.32.0200   zinc carboxypeptidase, putative,metallo-peptidase, Clan MC, Family M14
Loa Loa (eye worm) LOAG_08804   hypothetical protein
Mus musculus ENSMUSG00000038836   ATP/GTP binding protein-like 3
Mus musculus 244071   ATP/GTP binding protein-like 1
Mus musculus ENSMUSG00000021557   ATP/GTP binding protein 1
Mus musculus ENSMUSG00000040812   ATP/GTP binding protein-like 2
Neospora caninum NCLIV_039720   zinc carboxypeptidase, putative
Onchocerca volvulus OVOC11748   Cytosolic carboxypeptidase 1 homolog
Plasmodium berghei PBANKA_0209700   zinc-carboxypeptidase, putative
Plasmodium falciparum PF3D7_0103400   zinc-carboxypeptidase, putative
Plasmodium knowlesi PKNH_0210100   zinc-carboxypeptidase, putative
Plasmodium vivax PVX_081585   hypothetical protein, conserved
Plasmodium yoelii PY03811   Homo sapiens KIAA1035 protein-related
Schistosoma japonicum Sjp_0004350   ko:K01290 carboxypeptidase A [EC3.4.17.1], putative
Schistosoma japonicum Sjp_0113610   Cytosolic carboxypeptidase 1, putative
Schistosoma mansoni Smp_167640   peptidase
Trypanosoma brucei gambiense Tbg972.10.13020   zinc carboxypeptidase, putative,metallo- peptidase, Clan MC, Family M14
Trypanosoma brucei Tb927.10.10750   metallo-peptidase, Clan MC, Family M14, putative
Trypanosoma congolense TcIL3000_10_9050   metallo-peptidase, Clan MC, Family M14, putative
Trypanosoma cruzi TcCLB.503583.10   metallo-peptidase, Clan MC, Family M14, putative
Trypanosoma cruzi TcCLB.503489.10   metallo-peptidase, Clan MC, Family M14, putative
Toxoplasma gondii TGME49_265780   flagellar/basal body protein
Trichomonas vaginalis TVAG_027310   Clan MC, family M14, Zinc carboxypeptidase-like metallopeptidase
Trichomonas vaginalis TVAG_483870   Clan MC, family M14, Zinc carboxypeptidase-like metallopeptidase
Trichomonas vaginalis TVAG_021370   Clan MC, family M14, Zinc carboxypeptidase-like metallopeptidase
Trichomonas vaginalis TVAG_247150   Clan MC, family M14, Zinc carboxypeptidase-like metallopeptidase
Trichomonas vaginalis TVAG_453960   Clan MC, family M14, Zinc carboxypeptidase-like metallopeptidase
Trichomonas vaginalis TVAG_045700   Clan MC, family M14, Zinc carboxypeptidase-like metallopeptidase

Essentiality

TcCLB.503489.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.10750 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.10750 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.10750 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.10750 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0209700 Plasmodium berghei Dispensable plasmo
TGME49_265780 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens ATP/GTP binding protein-like 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.503489.10 (Trypanosoma cruzi), metallo-peptidase, Clan MC, Family M14, putative
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