Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Help me make sense of these data.

PDB Structures:

• 1NW4:

• Resolution	Method	# Atoms	# Residues	Dep. Date	Pub. Date	Mod. Date

• 1Q1G:

• Resolution	Method	# Atoms	# Residues	Dep. Date	Pub. Date	Mod. Date

• 1SQ6:

• Resolution	Method	# Atoms	# Residues	Dep. Date	Pub. Date	Mod. Date

• 2B94:

• Resolution	Method	# Atoms	# Residues	Dep. Date	Pub. Date	Mod. Date

• 2BSX:

• Resolution	Method	# Atoms	# Residues	Dep. Date	Pub. Date	Mod. Date

• 3EMV:

• Resolution	Method	# Atoms	# Residues	Dep. Date	Pub. Date	Mod. Date

• 3ENZ:

• Resolution	Method	# Atoms	# Residues	Dep. Date	Pub. Date	Mod. Date

• 3FOW:

• Resolution	Method	# Atoms	# Residues	Dep. Date	Pub. Date	Mod. Date

• 3PHC:

• Resolution	Method	# Atoms	# Residues	Dep. Date	Pub. Date	Mod. Date

• 6AQS:

• Resolution	Method	# Atoms	# Residues	Dep. Date	Pub. Date	Mod. Date

• 6AQU:

• Resolution	Method	# Atoms	# Residues	Dep. Date	Pub. Date	Mod. Date

Assay information

• Assay for Nucleoside Phosphorylase (2.4.2.1 ) Sigma-Aldrich
• Automatic link to known assays based on EC numbers.
• Inhibition of Plasmodium falciparum PNP ChEMBL
• Inhibition of Plasmodium falciparum PNP
• ChEMBL
• BindingDB_Patents: Inhibition Assay. The inhibitor dissociation constants reported in Table 1 below are for phosphorolysis of inosine by PNP and were based on reaction rates measurements with different inhibitor concentrations. Reactions were started by addition of 0.05 ug of human or Plasmodium falciparum purine nucleoside phosphaorylase (HsPNP and PfPNP, respectively; final concentration 1.4 nM) to 1 mM inosine in 50 mM KPO4, pH=7.5 buffer with xanthine oxidase added to final concentration 60 mU/mL at 25 C. In the coupled assay, hypoxanthine formed by phosphorolysis of inosine was oxidized to uric acid and followed spectrophotometrically at 293 nm (extinction coefficient for uric acid epsilon(293)=12.9 mM-1). The dissociation constant for slow-onset tight-binding inhibitors was determined from reaction rates after slow onset inhibition had occurred according to the equation u =(kcat x S)/(Km(1+l/Kd)+S), where u  is the steady state reaction rate after the slow-onset inhibition period has reached equilibrium.
• ChEMBL
• BindingDB_Patents: ADP-Glo Kinase Assay. Inhibitor Concentration at 50% enzyme inhibition (IC50) values were calculated by quantifying the end-point ADP production from each kinase reaction using the ADP-Glo Kinase Assay (Promega, Madison, Wis.) as described by the manufacturer. Reactions were performed in Tris buffer (50 mM pH 7.5, RT) containing 20 mM MgCl2 and 0.1% Bovine Serum Albumin. Each assay was performed in 60 uL of the solution in 10x75 mm borosilicate glass test tubes and allowed to continuously shake during the duration of the assay. The total ADP generated was quantified by transferring 25 uL (2x) of each assay to a 96-well luminescence assay plate, followed by the addition of 25 uL of ADP-Glo Reagent (45 min incubation) to remove any remaining ATP. For luminescence readings, 50 uL of Kinase Detection Reagent (45 min incubation) was added to convert the ADP generated from the kinase reaction to ATP, and luminescent intensity was measured using a luciferase/luciferin reaction.
• ChEMBL
• Inhibition of Plasmodium falciparum purine nucleoside phosphorylase assessed as slow onset inhibition constant by xanthine-oxidase coupled assay
• ChEMBL
• Inhibition of Plasmodium falciparum purine nucleoside phosphorylase by xanthine-oxidase coupled assay
• ChEMBL
• Inhibition of Plasmodium falciparum PNP expressed in Escherichia coli BL21(DE3) cells by spectrophotometry
• ChEMBL
• Inhibition of recombinant Plasmodium falciparum PNP using inosine as substrate by xanthine oxidase coupling enzyme assay
• ChEMBL
• Inhibition of recombinant Plasmodium falciparum PNP using inosine as substrate assessed as inhibition constant for slow onset inhibition of enzyme-inhibitor complex by xanthine oxidase coupling enzyme assay
• BRENDA Assay
• An enzyme with this EC number or name or sequence has been assayed in Plasmodium falciparum ( 2 )

Reagent availability

• Pfal008421AAA;

• Type	Source	Notes
  soluble recombinant protein	Structural Genomics for Pathogenic Protozoa (SGPP)	Pfal008421; Recombinant protein: full-length; Source: P falciparum; uridine phosphorylase, putative ;

• Reagent:

• Target	Type	Source	Notes
  PF3D7_0513300	purified protein	BRENDA	A protein with this EC number or name or sequence has been purified from Plasmodium lophurae ( 1 )