Detailed view for LbrM.21.1240

Basic information

TDR Targets ID: 429409
Leishmania braziliensis, epsilon tubulin, putative
Source Database / ID: 

pI: 6.5857 | Length (AA): 470 | MW (Da): 51881 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00091   Tubulin/FtsZ family, GTPase domain
PF03953   Tubulin C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005874   microtubule  
GO:0005525   GTP binding  
GO:0003924   GTPase activity  
GO:0007017   microtubule-based process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 449 1z5v (A) 2 436 29.00 0 1 1.22679 -0.3
2 462 3cb2 (A) 2 446 27.00 0 1 1.23585 -0.33
3 459 2btq (A) 4 439 27.00 0 1 1.27094 -0.75
3 457 4i4t (D) 2 441 30.00 0 1 1.33109 -0.71
3 448 1ffx (B) 2 432 34.00 0 1 1.16114 0.6

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_130726)

Species Accession Gene Product
Echinococcus granulosus EgrG_000603100   tubulin epsilon chain
Echinococcus multilocularis EmuJ_000603100   tubulin epsilon chain
Giardia lamblia GL50803_6336   Epsilon tubulin
Homo sapiens ENSG00000074935   tubulin, epsilon 1
Leishmania braziliensis LbrM.21.1240   epsilon tubulin, putative
Leishmania donovani LdBPK_211250.1   epsilon tubulin, putative
Leishmania infantum LinJ.21.1250   epsilon tubulin, putative
Leishmania major LmjF.21.1010   epsilon tubulin, putative
Leishmania mexicana LmxM.21.1010   epsilon tubulin, putative
Mus musculus ENSMUSG00000019845   epsilon-tubulin 1
Neospora caninum NCLIV_007070   tubulin epsilon chain, putative
Plasmodium berghei PBANKA_1300500   tubulin epsilon chain, putative
Plasmodium falciparum PF3D7_1475700   tubulin epsilon chain, putative
Plasmodium knowlesi PKNH_1246000   tubulin epsilon chain, putative
Plasmodium vivax PVX_118660   tubulin epsilon chain, putative
Plasmodium yoelii PY05777   tubulin beta chain
Schistosoma japonicum Sjp_0009290   ko:K10391 tubulin epsilon, putative
Schistosoma mansoni Smp_028360   tubulin subunit epsilon
Schmidtea mediterranea mk4.013167.01   Tubulin epsilon chain
Schmidtea mediterranea mk4.013167.02   Tubulin epsilon chain, putative
Schmidtea mediterranea mk4.016956.01   Tubulin epsilon chain, putative
Schmidtea mediterranea mk4.023212.00   Tubulin epsilon chain, putative
Schmidtea mediterranea mk4.016956.00  
Trypanosoma brucei gambiense Tbg972.10.1240   epsilon tubulin
Trypanosoma brucei Tb927.10.1140   epsilon tubulin
Trypanosoma brucei Tb11.0530   epsilon tubulin
Trypanosoma congolense TcIL3000_10_940   epsilon tubulin
Trypanosoma cruzi TcCLB.509967.160   epsilon tubulin, putative
Trypanosoma cruzi TcCLB.509695.120   epsilon tubulin, putative
Toxoplasma gondii TGME49_275970   tubulin, putative
Toxoplasma gondii TGME49_275870   tubulin/FtsZ family, GTPase domain-containing protein
Trichomonas vaginalis TVAG_065740   tubulin gamma chain, putative

Essentiality

LbrM.21.1240 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.1140 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.1140 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.1140 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.1140 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1300500 Plasmodium berghei Dispensable plasmo
TGME49_275870 Toxoplasma gondii Probably non-essential sidik
TGME49_275970 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Leishmania major epsilon tubulin, putative Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Trypanosoma brucei alpha tubulin 451 aa 29.4% 472 aa Compounds References
Leishmania major alpha tubulin 451 aa 29.0% 472 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Leishmania major alpha tubulin 451 aa 29.0% 472 aa Compounds References
Leishmania major alpha tubulin 451 aa 29.0% 472 aa Compounds References
Bos taurus Tubulin 85 aa 30.3% 109 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Leishmania major alpha tubulin 451 aa 29.0% 472 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Leishmania major alpha tubulin 451 aa 29.0% 472 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Leishmania major alpha tubulin 451 aa 29.0% 472 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Trypanosoma brucei beta tubulin 442 aa 29.2% 465 aa Compounds References
Trypanosoma brucei alpha tubulin 451 aa 29.4% 472 aa Compounds References
Sus scrofa Tubulin 445 aa 29.9% 465 aa Compounds References
Leishmania major alpha tubulin 451 aa 29.0% 472 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Leishmania major alpha tubulin 451 aa 29.0% 472 aa Compounds References
Sus scrofa Tubulin alpha chain 451 aa 29.4% 469 aa Compounds References
Leishmania major alpha tubulin 451 aa 29.0% 472 aa Compounds References
Trypanosoma brucei tubulin gamma chain 447 aa 28.8% 486 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Plasmodium falciparum alpha tubulin 1 453 aa 29.7% 472 aa Compounds References
Leishmania major alpha tubulin 451 aa 29.0% 472 aa Compounds References
Leishmania major alpha tubulin 451 aa 29.0% 472 aa Compounds References
Trypanosoma brucei alpha tubulin 451 aa 29.4% 472 aa Compounds References
Rattus norvegicus Tubulin alpha-1 chain 451 aa 29.4% 469 aa Compounds References
Trypanosoma brucei alpha tubulin 451 aa 29.4% 472 aa Compounds References
Ovis aries Tubulin alpha 4a 448 aa 29.6% 469 aa Compounds References
Leishmania major alpha tubulin 451 aa 29.0% 472 aa Compounds References
Plasmodium falciparum tubulin beta chain 445 aa 30.6% 467 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Bos taurus Tubulin beta chain 445 aa 30.1% 465 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Leishmania major beta tubulin 443 aa 30.4% 464 aa Compounds References
Bos taurus Tubulin 445 aa 30.1% 465 aa Compounds References
Leishmania major gamma-tubulin 447 aa 28.7% 485 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LbrM.21.1240 (Leishmania braziliensis), epsilon tubulin, putative
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