Detailed view for LbrM.29.2120

Basic information

TDR Targets ID: 430361
Leishmania braziliensis, C-8 sterol isomerase-like protein
Source Database / ID: 

pI: 6.3308 | Length (AA): 223 | MW (Da): 24848 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04622   ERG2 and Sigma1 receptor like protein

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Steroid biosynthesis (KEGG)
Global map (KEGG)
Biosynthesis of antibiotics (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
8 218 5hk1 (A) 12 214 31.00 0 0.6 1.25289 0.08

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_131051)

Species Accession Gene Product
Brugia malayi Bm1_43605   ERG2 and Sigma1 receptor like protein
Candida albicans CaO19.6026   sterol C8-C7 isomerase
Candida albicans CaO19.13447   sterol C8-C7 isomerase
Caenorhabditis elegans CELE_W08F4.3   Protein W08F4.3
Dictyostelium discoideum DDB_G0270356   C-8 sterol isomerase
Homo sapiens ENSG00000147955   sigma non-opioid intracellular receptor 1
Leishmania braziliensis LbrM.29.2120   C-8 sterol isomerase-like protein
Leishmania donovani LdBPK_292250.1   C-8 sterol isomerase-like protein
Leishmania infantum LinJ.29.2250   C-8 sterol isomerase-like protein
Leishmania major LmjF.29.2140   C-8 sterol isomerase-like protein
Leishmania mexicana LmxM.08_29.2140   C-8 sterol isomerase-like protein
Loa Loa (eye worm) LOAG_09056   hypothetical protein
Loa Loa (eye worm) LOAG_11447   hypothetical protein
Mus musculus ENSMUSG00000036078   sigma non-opioid intracellular receptor 1
Saccharomyces cerevisiae YMR202W   C-8 sterol isomerase ERG2
Trypanosoma brucei gambiense Tbg972.3.5180   C-8 sterol isomerase, putative
Trypanosoma brucei Tb927.3.4650   C-8 sterol isomerase, putative
Trypanosoma congolense TcIL3000_0_39140   C-8 sterol isomerase, putative
Trypanosoma congolense TcIL3000_3_2850   C-8 sterol isomerase, putative
Trypanosoma cruzi TcCLB.510329.90   C-8 sterol isomerase, putative

Essentiality

LbrM.29.2120 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.4650 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.4650 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.4650 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.4650 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens sigma non-opioid intracellular receptor 1 Compounds References
Mus musculus sigma non-opioid intracellular receptor 1 Compounds References
Cavia porcellus Sigma-1 receptor Compounds References
Saccharomyces cerevisiae C-8 sterol isomerase ERG2 Compounds References
Rattus norvegicus Sigma opioid receptor Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LbrM.29.2120 (Leishmania braziliensis), C-8 sterol isomerase-like protein
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