Detailed view for TcCLB.508111.30

Basic information

TDR Targets ID: 43836
Trypanosoma cruzi, glutamate dehydrogenase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.9187 | Length (AA): 416 | MW (Da): 45057 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00208   Glutamate/Leucine/Phenylalanine/Valine dehydrogenase
PF02812   Glu/Leu/Phe/Val dehydrogenase, dimerisation domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016639   oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor  
GO:0016491   oxidoreductase activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  
GO:0006520   amino acid metabolic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 416 1hwx (A) 10 449 25.00 0 1 1.08 -0.05
1 415 2bma (A) 29 447 55.00 0 1 1.61 -1.2
1 415 1bgv (A) 29 447 55.00 0 1 1.75 -1.16
1 416 4fcc (A) 32 447 71.00 0 1 1.9266 -1.26

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126857)

Species Accession Gene Product
Arabidopsis thaliana AT5G07440   glutamate dehydrogenase 2
Arabidopsis thaliana AT1G51720   amino acid dehydrogenase family protein
Arabidopsis thaliana AT5G18170   glutamate dehydrogenase 1
Arabidopsis thaliana AT3G03910   glutamate dehydrogenase 3
Babesia bovis BBOV_IV010390   glutamate dehydrogenase, putative
Brugia malayi Bm1_08210   glutamate dehydrogenase, mitochondrial precursor, putative
Brugia malayi Bm1_13615   glutamate dehydrogenase, mitochondrial precursor, putative
Candida albicans CaO19.4716   similar to S. cerevisiae NADP-specific glutamate dehydrogenase GDH1 (YOR375C)
Caenorhabditis elegans CELE_ZK829.4   Protein GDH-1
Dictyostelium discoideum DDB_G0287469   NAD-dependent glutamate dehydrogenase
Drosophila melanogaster Dmel_CG4434   CG4434 gene product from transcript CG4434-RA
Drosophila melanogaster Dmel_CG5320   Glutamate dehydrogenase
Escherichia coli b1761   glutamate dehydrogenase, NADP-specific
Echinococcus granulosus EgrG_000604400   Glutamate dehydrogenase mitochondrial
Echinococcus granulosus EgrG_000589100   glutamate dehydrogenase
Echinococcus multilocularis EmuJ_000589100   glutamate dehydrogenase
Echinococcus multilocularis EmuJ_000604400   Glutamate dehydrogenase, mitochondrial
Giardia lamblia GL50803_21942   NADP-specific glutamate dehydrogenase
Homo sapiens ENSG00000148672   glutamate dehydrogenase 1
Homo sapiens ENSG00000182890   glutamate dehydrogenase 2
Leishmania braziliensis LbrM.28.3120   glutamate dehydrogenase, putative
Leishmania donovani LdBPK_283140.1   glutamate dehydrogenase, putative
Leishmania infantum LinJ.28.3140   glutamate dehydrogenase, putative
Leishmania major LmjF.28.2910   glutamate dehydrogenase, putative
Leishmania mexicana LmxM.28.2910   glutamate dehydrogenase, putative
Mus musculus ENSMUSG00000021794   glutamate dehydrogenase 1
Neospora caninum NCLIV_000130   hypothetical protein
Oryza sativa 4324672   Os01g0558200
Oryza sativa 4330164   Os02g0650900
Oryza sativa 4336554   Os04g0543900
Oryza sativa 4334405   Os03g0794500
Onchocerca volvulus OVOC9031   Glutamate dehydrogenase, mitochondrial homolog
Onchocerca volvulus OVOC8679   Glutamate dehydrogenase, mitochondrial homolog
Plasmodium berghei PBANKA_1026200   NADP-specific glutamate dehydrogenase, putative
Plasmodium berghei PBANKA_1014000   NADP-specific glutamate dehydrogenase, putative
Plasmodium falciparum PF3D7_1430700   NADP-specific glutamate dehydrogenase
Plasmodium falciparum PF3D7_1416500   NADP-specific glutamate dehydrogenase
Plasmodium knowlesi PKNH_1341600   NADP-specific glutamate dehydrogenase, putative
Plasmodium knowlesi PKNH_1328000   NADP-specific glutamate dehydrogenase, putative
Plasmodium vivax PVX_085625   glutamate dehydrogenase (NADP+), putative
Plasmodium yoelii PY04261   glutamate dehydrogenase
Plasmodium yoelii PY01264   glutamate dehydrogenase
Saccharomyces cerevisiae YOR375C   glutamate dehydrogenase (NADP(+)) GDH1
Saccharomyces cerevisiae YAL062W   glutamate dehydrogenase (NADP(+)) GDH3
Schistosoma japonicum Sjp_0114080   ko:K00261 glutamate dehydrogenase (NAD(P)+) [EC1.4.1.3], putative
Schistosoma mansoni Smp_093820   glutamate dehydrogenase
Schmidtea mediterranea mk4.001394.08   Glutamate dehydrogenase
Schmidtea mediterranea mk4.001394.07   Glutamate dehydrogenase, mitochondrial
Trypanosoma cruzi TcCLB.508111.30   glutamate dehydrogenase, putative
Trypanosoma cruzi TcCLB.507875.20   glutamate dehydrogenase, putative
Toxoplasma gondii TGME49_293180   NADP-specific glutamate dehydrogenase
Trichomonas vaginalis TVAG_072100   glutamate dehydrogenase, putative
Trichomonas vaginalis TVAG_025910   glutamate dehydrogenase, putative
Trichomonas vaginalis TVAG_201620   glutamate dehydrogenase, putative
Trichomonas vaginalis TVAG_025980   glutamate dehydrogenase, putative
Trichomonas vaginalis TVAG_196700   glutamate dehydrogenase, putative

Essentiality

TcCLB.508111.30 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b1761 Escherichia coli non-essential goodall
CELE_ZK829.4 Caenorhabditis elegans slow growth wormbase
CELE_ZK829.4 Caenorhabditis elegans sterile wormbase
PBANKA_1014000 Plasmodium berghei Slow plasmo
TGME49_293180 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Bos taurus Glutamate dehydrogenase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma cruzi ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    TcCLB.508111.30 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma cruzi ( 1 )

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.508111.30 (Trypanosoma cruzi), glutamate dehydrogenase, putative
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