Detailed view for TcCLB.506563.200

Basic information

TDR Targets ID: 47480
Trypanosoma cruzi, calpain cysteine peptidase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.8364 | Length (AA): 729 | MW (Da): 82630 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00648   Calpain family cysteine protease
PF09149   Domain of unknown function (DUF1935)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0004198   calcium-dependent cysteine-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
54 162 1r75 (A) 13 130 36.00 0 0.97 0.57 -1.12
161 723 1qxp (A) 8 625 21.00 0 1 0.73 0.36
177 514 1zcm (A) 33 353 27.00 0 1 0.72 -0.89
47 164 2fe0 (A) 6 126 53.00 0 1 0.776966 -0.52
55 148 1r75 (A) 14 116 41.00 0.000000000064 0.98 0.644744 -0.75
164 728 1df0 (A) 10 591 22.00 0 1 0.680134 0.89
177 514 1zcm (A) 33 353 28.00 0 1 0.790749 -0.63
190 526 1qxp (B) 33 357 33.00 0 1 0.703077 0.32

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_156130)

Species Accession Gene Product
Trypanosoma brucei gambiense Tbg972.1.1250   calpain, putative,cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei Tb927.1.2120   Calpain-like protein CALP1.3
Trypanosoma congolense TcIL3000_1_1200   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma cruzi TcCLB.506563.200   calpain cysteine peptidase, putative
Trypanosoma cruzi TcCLB.508999.220   cysteine peptidase, Clan CA, family C2, putative

Essentiality

TcCLB.506563.200 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.2120 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.1.2120 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.2120 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.2120 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 414 aa 34.1% 416 aa Compounds References
Leishmania major cytoskeleton-associated protein CAP5.5, putative,cysteine peptidase, Clan CA, family C2, putative 723 aa 35.3% 638 aa Compounds References
Leishmania major small myristoylated protein-1 131 aa 56.9% 109 aa Compounds References
Leishmania major small myristoylated protein-3, putative 115 aa 42.0% 112 aa Compounds References
Leishmania major small myristoylated protein-2 136 aa 44.8% 116 aa Compounds References
Leishmania major calpain family cysteine protease-like protein 705 aa 33.0% 667 aa Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 687 aa 39.9% 674 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0907978 0.536828 0.532274
0.0505 0.5763 0.5763
0.0492 0.5754 0.5754
0.05 0.5214 0.5819
0.0442 0.4771 0.5584
0.0543 0.5813 0.5796
0.0532 0.5737 0.5737
0.1124 0.5744 0.5737
0.0519 0.5783 0.5779
0.0511 0.3542 0.3617
0.0507 0.5783 0.5779
0.0317 0.3568 0.5728
0.0589 0.2514 0.2941
0.0495 0.3173 0.5288
0.0489 0.3134 0.5278
0.0581 0.5764 0.5763
0.0474 0.3334 0.5773
0.05 0.5754 0.5754
0.0641 0.482 0.5812
0.085 0.4182 0.5766
0.0488 0.5763 0.5763
0.0512 0.4013 0.575
0.0446 0.3582 0.3909
0.0502 0.5847 0.5847
0.0589 0.2514 0.2941
0.0487 0.4509 0.5595
0.0512 0.4013 0.575
0.0478 0.5793 0.5791
0.0383 0.2948 0.5309
0.0505 0.2553 0.254
0.1058 0.4249 0.583
0.0505 0.5763 0.5763
0.0483 0.3544 0.3955
0.085 0.4255 0.584
0.06 0.5899 0.5897
0.0467 0.2989 0.3734
0.0293 0.281 0.281
0.0512 0.2527 0.2521
0.1008 0.5744 0.5737
0.0446 0.4317 0.4723
0.0496 0.5818 0.5808
0.0533 0.5754 0.5754
0.0479 0.4725 0.5713
0.05 0.5818 0.5808
0.06 0.5767 0.5767
0.0514 0.5754 0.5754
0.0483 0.3502 0.5549
0.0481 0.5813 0.5808
0.0293 0.281 0.281
0.1116 0.4234 0.5819
0.0467 0.2542 0.2533
0.0523 0.5763 0.5763
0.0262 0.5595 0.5595
0.0551 0.5676 0.5791
0.0449 0.4945 0.5579
0.0516 0.5575 0.5701
0.05 0.3507 0.5838
0.0533 0.5756 0.5791
0.1116 0.5744 0.5737
0.0577 0.5821 0.5819
0.0551 0.5582 0.5791
0.0503 0.4335 0.5871
0.0461 0.4502 0.4978
0.0505 0.2799 0.5654
0.0545 0.2732 0.2714
0.0533 0.5754 0.5754
0.0492 0.5767 0.5763
0.0527 0.5794 0.5779
0.0558 0.2925 0.5856
0.0517 0.5707 0.5719
0.0564 0.5763 0.5763
0.0474 0.5249 0.5745
0.0425 0.4583 0.4582
0.0511 0.5794 0.5779
0.0493 0.5814 0.5813
0.0373 0.5673 0.5595
0.0561 0.3468 0.5779
0.06 0.5899 0.5897
0.0446 0.3582 0.3909
0.0575 0.5763 0.5763
0.0559 0.3227 0.3246
0.0565 0.5684 0.5674
0.0571 0.5793 0.5791
0.0533 0.2641 0.2628
0.0543 0.5773 0.5773
0.0529 0.5673 0.5659
0.0476 0.5283 0.5283
0.0566 0.5793 0.5791
0.0446 0.3582 0.3909
0.05 0.2767 0.5679
0.0442 0.4771 0.5584
0.0907978 0.536828 0.532274
0.0498 0.5754 0.5754
0.0457 0.4243 0.5697
0.049 0.3467 0.4466
0.0522 0.3983 0.5796
0.045 0.4319 0.5662
0.0293 0.281 0.281
0.057 0.4308 0.5779
0.0479 0.5763 0.5763
0.0449 0.4945 0.5579
0.0577 0.3348 0.5694
0.0293 0.281 0.281
0.0488 0.5763 0.5763
0.0521 0.5255 0.5754
0.0908 0.4281 0.5865
0.0528 0.572 0.5719
0.0532 0.3551 0.5779
0.0446 0.3582 0.3909
0.0487 0.5763 0.5763
0.0293 0.281 0.281
0.0437 0.2694 0.4189
0.0435 0.4348 0.4329
0.0589 0.259 0.2579
0.1116 0.5744 0.5737
0.0481 0.2716 0.5595
0.1008 0.5744 0.5737
0.1174 0.5754 0.5744
0.0381 0.2828 0.6297
0.0533 0.5818 0.5809
0.1183 0.5744 0.5737
0.0527 0.3148 0.5779
0.0435 0.4348 0.4329
0.1174 0.5744 0.5737
0.0457 0.4243 0.5697
0.05 0.3507 0.5838
0.0553197 0.518866 0.518866
0.0496 0.5796 0.5796
0.0503 0.3253 0.5711
0.0496 0.2617 0.2609
0.0525 0.572 0.5719
0.0523 0.5767 0.5728
0.0475 0.5664 0.5595
0.0587 0.5721 0.5719
0.1124 0.5744 0.5737
0.0908 0.4281 0.5865
0.05 0.5214 0.5819
0.0435 0.4479 0.5615
0.0533 0.5763 0.5763
0.0527 0.3148 0.5779
0.106603 0.536752 0.532274
0.0561 0.5973 0.5973
0.0849 0.5744 0.5737
0.0561 0.4352 0.5796
0.0446 0.4928 0.5584
0.05 0.5214 0.5819
0.0389 0.5297 0.5283
0.05 0.3507 0.5838
0.0492 0.317 0.5282
0.0587 0.566 0.5619
0.0446 0.4331 0.5515
0.0544 0.5461 0.5921
0.0576 0.5793 0.5791
0.0552 0.573 0.5814
0.0569 0.5754 0.5754
0.0464 0.428 0.5689
0.0493 0.3983 0.5622
0.0503 0.4335 0.5871
0.0577 0.5821 0.5819
0.0531153 0.378113 0.531612
0.0566 0.5792 0.5791
0.0582 0.5793 0.5791
0.1066 0.5744 0.5737
0.0293 0.281 0.281
0.0401 0.2745 0.5754
0.0518 0.5105 0.5722
0.1116 0.4234 0.5819
0.0582 0.5793 0.5791
0.059 0.3537 0.5711
0.0471 0.5763 0.5763
0.0434 0.3527 0.5592
0.0512 0.4013 0.575
0.0575 0.5754 0.5754
0.1057 0.5744 0.5737
0.0478 0.5793 0.5791
0.0474 0.5249 0.5745
0.0381 0.5847 0.5844
0.0317 0.3666 0.5657
0.051 0.5445 0.5717
0.0587 0.2642 0.5693
0.0523 0.5763 0.5763
0.0488 0.5763 0.5763
0.1116 0.4234 0.5819
0.1057 0.5744 0.5737
0.053 0.5691 0.5725
0.0489 0.5733 0.5653
0.0457 0.4243 0.5697
0.0545 0.3871 0.5017
0.0476 0.572 0.572
0.0581 0.3518 0.5737
0.0446 0.3582 0.3909
0.0527 0.32 0.5836
0.0434 0.4906 0.5588
0.1116 0.4234 0.5819
0.0542 0.2811 0.5779
0.0489 0.5779 0.5779
0.0446 0.4331 0.5515
0.1174 0.5737 0.5737
0.0478 0.5813 0.5808
0.0446 0.4342 0.5704
0.1174 0.5744 0.5737
0.0539 0.2704 0.4972
0.0472 0.5763 0.5763
0.0544 0.5818 0.5809
0.0511 0.5074 0.5808
0.0475 0.5766 0.5763
0.1174 0.5754 0.5744
0.0377 0.5756 0.5756
0.0481 0.5813 0.5808
0.0476 0.5283 0.5283
0.1058 0.4249 0.583
0.0498 0.3312 0.5719
0.0505 0.5754 0.5754
0.0476 0.5283 0.5283
0.0488 0.5818 0.5808
0.1187 0.5754 0.5744
0.1066 0.5744 0.5737
0.0559 0.3227 0.3246
0.0587 0.2642 0.5693
0.0553 0.5763 0.5763
0.0582 0.5872 0.5842
0.0575 0.5754 0.5754
0.0509 0.5883 0.588
0.0503 0.2633 0.2626
0.05 0.3507 0.5838
0.0475 0.5763 0.5763
0.0577 0.5821 0.5819
0.0488 0.5763 0.5763
0.1066 0.5744 0.5737
0.0519 0.5796 0.5796
0.0519 0.2864 0.3105
0.0293 0.281 0.281
0.0474 0.3045 0.5595
0.1183 0.5744 0.5737
0.0545 0.3871 0.5017
0.0561 0.5973 0.5973
0.0439 0.5186 0.5545
0.0543 0.5754 0.5754
0.0542 0.2811 0.5779
0.0479 0.4725 0.5713
0.0474 0.5249 0.5745
0.0503 0.3879 0.5779
0.1116 0.4249 0.583
0.0262 0.566 0.5614
0.085 0.4255 0.584
0.0571 0.5818 0.5809
0.0472 0.581 0.5791
0.057 0.2619 0.471
0.0542 0.2811 0.5779
0.057 0.2619 0.471
0.05 0.5783 0.5779
0.0447 0.3333 0.558
0.0435 0.4479 0.5615
0.0474 0.3334 0.5773
0.0509 0.5883 0.588
0.1116 0.4249 0.583
0.0571 0.5793 0.5791
0.0293 0.281 0.281
0.1124 0.5744 0.5737
0.0559 0.5795 0.5779
0.0589 0.2514 0.2941
0.0565 0.5684 0.5674
0.0561 0.5973 0.5973
0.0383 0.2948 0.5309
0.0483 0.5092 0.5793
0.0949 0.4249 0.583
0.0481 0.2716 0.5595
0.0512 0.5818 0.5808
0.0551 0.5676 0.5791
0.0266 0.5737 0.5737
0.1058 0.4249 0.583
0.0421 0.3368 0.5504
0.0503 0.3253 0.5711
0.1116 0.5744 0.5737
0.0476 0.5283 0.5283
0.0487 0.5679 0.5654
0.0498 0.5754 0.5754
0.0521 0.5255 0.5754
0.0377 0.5756 0.5756
0.04 0.4376 0.4351
0.0377 0.5744 0.5737
0.0561 0.4352 0.5796
0.0497 0.5208 0.5719
0.0501 0.5069 0.5712
0.0475 0.5766 0.5763
0.0474 0.3045 0.5595
0.0497 0.5208 0.5719
0.0512 0.4013 0.575
0.0504 0.5808 0.5808
0.06 0.5899 0.5897
0.0575 0.5754 0.5754
0.0471 0.5372 0.5819
0.0561 0.5922 0.5922
0.1183 0.5744 0.5737
0.1116 0.5744 0.5737
0.0587 0.579 0.5754
0.0589 0.2514 0.2941
0.0507 0.5813 0.5808
0.0524 0.5165 0.5723

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.506563.200 (Trypanosoma cruzi), calpain cysteine peptidase, putative
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