Detailed view for PF3D7_1209500

Basic information

TDR Targets ID: 5143
Plasmodium falciparum, cGMP-specific phosphodiesterase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.627 | Length (AA): 954 | MW (Da): 115412 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 6

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00233   3'5'-cyclic nucleotide phosphodiesterase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004114   3',5'-cyclic-nucleotide phosphodiesterase activity  
GO:0008081   phosphoric diester hydrolase activity  
GO:0007165   signal transduction  

Metabolic Pathways

Purine metabolism (KEGG)

Structural information

Modbase 3D models:

There are 14 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
61 340 2f84 (A) 1 291 18.00 0.000014 0.02 0.4 -0.75
457 812 1taz (A) 159 497 33.00 0 1 0.62 -0.55
514 812 1zkl (A) 153 452 30.00 0 1 0.93 -2.18
391 555 3ci6 (A) 3 162 11.00 0.043 0.01 0.129456 0.53
506 926 4wzi (B) 174 655 22.00 0 1 0.6835 -0.22
569 924 1taz (A) 159 497 33.00 0 1 0.499366 0.19
610 924 1so2 (C) 660 1073 27.00 0 1 0.509089 -0.58
613 927 1zkl (A) 140 455 28.00 0 1 0.783389 -1.23
376 866 3ibj (A) 389 900 18.00 0 1 0.671948 0.63
444 834 4wzi (B) 167 627 27.00 0 1 0.606841 0.48
507 862 1taz (A) 159 497 33.00 0 1 0.526603 0.19
551 865 1zkl (A) 140 455 28.00 0 1 0.811939 -1.23
561 866 2r8q (A) 618 929 24.00 0 1 0.736549 -1.19
665 829 4qts (A) 38 221 28.00 0.12 0.14 0.123478 1.92

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 48 hs, Sporozoite. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 40 hs, late schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile gametocyte, early schizont, Male Gametocyte. PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, Oocyst, Female Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 16 hs, Ring. Otto TD Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_152655)

Species Accession Gene Product
Babesia bovis BBOV_IV010070   3'5'-cyclic nucleotide phosphodiesterase, putative
Plasmodium berghei PBANKA_0607900   3',5'-cyclic nucleotide phosphodiesterase, putative
Plasmodium falciparum PF3D7_1209500   cGMP-specific phosphodiesterase
Plasmodium knowlesi PKNH_1309400   3',5'-cyclic nucleotide phosphodiesterase, putative
Plasmodium vivax PVX_084505   3',5'-cyclic nucleotide phosphodiesterase, putative
Plasmodium yoelii PY01829   cGMP phosphodiesterase A4
Theileria parva TP01_0664   3',5' cyclic nucleotide phosphodiesterase, putative

Essentiality

No essentiality data collected for this gene and/or its orthologs.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) multi-cellular organism (CARO:0000012) bloodstream stage (PLO:0040) in vivo inhibition (TDR:00016) Plasmodium falciparum 38783  
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 16038615
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Plasmodium falciparum 38783  
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 16038615

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Phosphodiesterase, 3' 5'-Cyclic Nucleotide Crude Complex (3.1.4.17 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Plasmodium falciparum ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    PF3D7_1209500 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Plasmodium falciparum ( 1 )

Bibliographic References

3 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_1209500 (Plasmodium falciparum), cGMP-specific phosphodiesterase
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