Detailed view for TcCLB.507019.40

Basic information

TDR Targets ID: 53748
Trypanosoma cruzi, histidyl-tRNA synthetase
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.8028 | Length (AA): 478 | MW (Da): 53406 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03129   Anticodon binding domain
PF13393   Histidyl-tRNA synthetase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0004821   histidine-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0000166   nucleotide binding  
GO:0005524   ATP binding  
GO:0006427   histidyl-tRNA aminoacylation  
GO:0006418   tRNA aminoacylation for protein translation  
GO:0006412   translation  

Metabolic Pathways

Histidine metabolism (KEGG)
Aminoacyl-tRNA biosynthesis (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
48 477 1wu7 (A) 3 425 32.00 0 1 1.2 -0.34
49 464 1wu7 (A) 4 413 38.00 0 1 1.39 -0.74
382 477 1v95 (A) 7 108 22.00 0 1 0.51 -1.04
47 478 3lc0 (A) 47 478 99.99 0 1 2.03177 -0.74
48 456 3hri (F) 47 455 87.00 0 1 1.68565 0.14

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3HRK:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3LC0:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YP0:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YPF:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRC:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRE:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRF:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRG:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRI:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRJ:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRK:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRL:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRM:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRN:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRO:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRP:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRQ:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRR:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRS:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4YRT:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126806)

Species Accession Gene Product
Arabidopsis thaliana AT3G46100   Histidyl-tRNA synthetase 1
Arabidopsis thaliana AT3G02760   histidyl-tRNA synthetase
Babesia bovis BBOV_IV001190   histidyl-tRNA synthetase, putative
Babesia bovis BBOV_III005180   hypothetical protein
Brugia malayi Bm1_21685   histidyl-tRNA synthetase
Candida albicans CaO19.11533   histidyl-tRNA synthetase
Candida albicans CaO19.4051   histidyl-tRNA synthetase
Caenorhabditis elegans CELE_T11G6.1   Protein HARS-1, isoform B
Cryptosporidium hominis Chro.80461   histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA ligase) (HisRS)
Cryptosporidium parvum cgd8_4010   histidyl-tRNA synthetase
Chlamydia trachomatis CT_543   histidine--tRNA ligase
Dictyostelium discoideum DDB_G0269822   histidine-tRNA ligase
Dictyostelium discoideum DDB_G0274159   histidine-tRNA ligase
Drosophila melanogaster Dmel_CG6335   Histidyl-tRNA synthetase
Escherichia coli b2514   histidyl tRNA synthetase
Echinococcus granulosus EgrG_001008100   histidyl tRNA synthetase
Entamoeba histolytica EHI_180020   histidyl-tRNA synthetase, putative
Echinococcus multilocularis EmuJ_001008100   histidyl tRNA synthetase
Giardia lamblia GL50803_22808   Histidyl-tRNA synthetase
Homo sapiens ENSG00000112855   histidyl-tRNA synthetase 2, mitochondrial
Homo sapiens ENSG00000170445   histidyl-tRNA synthetase
Leishmania braziliensis LbrM.30.0710   histidyl-tRNA synthetase, putative
Leishmania donovani LdBPK_300650.1   histidyl-tRNA synthetase, putative
Leishmania infantum LinJ.30.0650   histidyl-tRNA synthetase, putative
Leishmania major LmjF.30.0630   histidyl-tRNA synthetase, putative
Leishmania mexicana LmxM.29.0630   histidyl-tRNA synthetase, putative
Loa Loa (eye worm) LOAG_05136   histidyl-tRNA synthetase
Mycobacterium leprae ML0494   Probable histidyl-tRNA synthase HisS (Histidine--tRNA ligase) (HisRS) (Histidine--Translase)
Mus musculus ENSMUSG00000019143   histidyl-tRNA synthetase 2, mitochondrial (putative)
Mus musculus ENSMUSG00000001380   histidyl-tRNA synthetase
Mycobacterium tuberculosis Rv2580c   Probable histidyl-tRNA synthetase HisS (histidine--tRNA ligase) (HISRS) (histidine--translase)
Mycobacterium ulcerans MUL_1732   histidyl-tRNA synthetase
Neospora caninum NCLIV_019550   histidyl-tRNA synthetase, putative
Oryza sativa 4330767   Os02g0754700
Oryza sativa 4337830   Os05g0150900
Plasmodium berghei PBANKA_0834800   histidine--tRNA ligase, putative
Plasmodium berghei PBANKA_1308900   histidine--tRNA ligase, putative
Plasmodium falciparum PF3D7_0934000   histidine--tRNA ligase, putative
Plasmodium falciparum PF3D7_1445100   histidine--tRNA ligase, putative
Plasmodium knowlesi PKNH_0732700   histidine--tRNA ligase, putative
Plasmodium knowlesi PKNH_1237200   histidine--tRNA ligase, putative
Plasmodium vivax PVX_087015   histidine--tRNA ligase, putative
Plasmodium vivax PVX_118235   histidine--tRNA ligase, putative
Plasmodium yoelii PY03706   histidyl-tRNA synthetase, putative
Plasmodium yoelii PY00739   CCAAT-box DNA binding protein subunit B
Saccharomyces cerevisiae YPR033C   histidine--tRNA ligase
Schistosoma japonicum Sjp_0201330   expressed protein
Schistosoma japonicum Sjp_0007710   ko:K01892 histidyl-tRNA synthetase [EC6.1.1.21S], putative
Schistosoma mansoni Smp_023360.1   histidyl-tRNA synthetase
Schistosoma mansoni Smp_023360.2   histidyl-tRNA synthetase
Schmidtea mediterranea mk4.000648.09   Probable histidineALQ-tRNA ligase, mitochondrial
Schmidtea mediterranea mk4.006253.02   Probable histidineALQ-tRNA ligase, mitochondrial
Schmidtea mediterranea mk4.033250.01   Probable histidineALQ-tRNA ligase, mitochondrial
Trypanosoma brucei gambiense Tbg972.6.1800   histidyl-tRNA synthetase, putative
Trypanosoma brucei Tb927.6.2060   histidyl-tRNA synthetase
Trypanosoma congolense TcIL3000_0_25100   histidyl-tRNA synthetase
Trypanosoma congolense TcIL3000_6_1720   histidyl-tRNA synthetase
Trypanosoma cruzi TcCLB.507019.40   histidyl-tRNA synthetase
Toxoplasma gondii TGME49_280600   histidyl-tRNA synthetase (HisRS), putative
Treponema pallidum TP0641   histidyl-tRNA synthetase
Theileria parva TP01_0145   histidyl-tRNA synthetase, putative
Theileria parva TP02_0435   histidyl-tRNA synthetase, putative
Trichomonas vaginalis TVAG_342610   interferon-induced, double-stranded RNA-activated protein kinase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0412   histidyl-tRNA synthetase

Essentiality

TcCLB.507019.40 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.2060 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.2060 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.2060 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.2060 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b2514 Escherichia coli essential goodall
CELE_T11G6.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_T11G6.1 Caenorhabditis elegans larval arrest wormbase
CELE_T11G6.1 Caenorhabditis elegans slow growth wormbase
CELE_T11G6.1 Caenorhabditis elegans sterile wormbase
YPR033C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1308900 Plasmodium berghei Essential plasmo
TGME49_280600 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens histidyl-tRNA synthetase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.507019.40 (Trypanosoma cruzi), histidyl-tRNA synthetase
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