Detailed view for TcCLB.508837.189

Basic information

TDR Targets ID: 53982
Trypanosoma cruzi, endoplasmic reticulum oxidoreductin, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.0609 | Length (AA): 443 | MW (Da): 50181 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04137   Endoplasmic Reticulum Oxidoreductin 1 (ERO1)

Gene Ontology

Mouse over links to read term descriptions.
GO:0016671   oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor  
GO:0003756   protein disulfide isomerase activity  
GO:0005789   endoplasmic reticulum membrane  
GO:0005783   endoplasmic reticulum  
GO:0050660   FAD binding  
GO:0009055   electron carrier activity  
GO:0005515   protein binding  
GO:0055114   oxidation reduction  
GO:0006467   protein thiol-disulfide exchange  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
63 437 1rp4 (A) 55 422 25.00 0 1 0.99 -0.81
36 436 3ahq (A) 35 465 32.00 0 1 1.04359 -0.2
60 431 3ahq (A) 46 463 37.00 0 1 1.03983 0
63 437 1rp4 (A) 55 422 27.00 0 1 1.0394 -0.36
63 435 3m31 (A) 55 420 28.00 0 1 1.12539 -0.35

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127747)

Species Accession Gene Product
Arabidopsis thaliana AT1G72280   endoplasmic oxidoreductin-1
Arabidopsis thaliana AT2G38960   endoplasmic oxidoreductin-2
Babesia bovis BBOV_IV002420   endoplasmic reticulum oxidoreductin, putative
Brugia malayi Bm1_47995   Endoplasmic Reticulum Oxidoreductin 1
Candida albicans CaO19.4871   protein disulfide bond formation in the ER
Candida albicans CaO19.12335   protein disulfide bond formation in the ER
Caenorhabditis elegans CELE_Y105E8B.8   Protein ERO-1, isoform B
Cryptosporidium hominis Chro.80371   hypothetical protein
Cryptosporidium parvum cgd8_3190   endoplasmic reticulum oxidoreductin
Dictyostelium discoideum DDB_G0288849   hypothetical protein
Drosophila melanogaster Dmel_CG1333   Endoplasmic reticulum oxidoreductin-1-like
Echinococcus granulosus EgrG_000245100   Endoplasmic reticulum oxidoreductin 1
Echinococcus multilocularis EmuJ_000245100   Endoplasmic reticulum oxidoreductin 1
Homo sapiens ENSG00000086619   ERO1-like beta (S. cerevisiae)
Homo sapiens 30001   ERO1-like (S. cerevisiae)
Leishmania braziliensis LbrM.16.1590   endoplasmic reticulum oxidoreductin, putative
Leishmania donovani LdBPK_161630.1   endoplasmic reticulum oxidoreductin, putative
Leishmania infantum LinJ.16.1630   endoplasmic reticulum oxidoreductin, putative
Leishmania major LmjF.16.1530   endoplasmic reticulum oxidoreductin, putative
Leishmania mexicana LmxM.16.1530   endoplasmic reticulum oxidoreductin, putative
Loa Loa (eye worm) LOAG_00621   hypothetical protein
Mus musculus ENSMUSG00000021831   ERO1-like (S. cerevisiae)
Mus musculus ENSMUSG00000057069   ERO1-like beta (S. cerevisiae)
Neospora caninum NCLIV_060300   cDNA, FLJ96396, highly similar to Homo sapiens ERO1-like beta (S. cerevisiae) (ERO1LB), mRNA, related
Oryza sativa 4334011   Os03g0733800
Plasmodium berghei PBANKA_0924300   endoplasmic reticulum oxidoreductin, putative
Plasmodium falciparum PF3D7_1124000   endoplasmic reticulum oxidoreductin, putative
Plasmodium knowlesi PKNH_0921800   endoplasmic reticulum oxidoreductin, putative
Plasmodium vivax PVX_091815   endoplasmic reticulum oxidoreductin, putative
Plasmodium yoelii PY07009   hypothetical protein
Saccharomyces cerevisiae YML130C   Ero1p
Schistosoma japonicum Sjp_0204470   ko:K00391 ERO1-like [EC:1.8.4.-], putative
Schistosoma mansoni Smp_124920   ero1-related
Schmidtea mediterranea mk4.001337.09   Endoplasmic reticulum oxidoreductin-1
Trypanosoma brucei gambiense Tbg972.8.4700   endoplasmic reticulum oxidoreductin, putative,pol-associated gene 1
Trypanosoma brucei Tb927.8.4890   pol-associated gene 1
Trypanosoma congolense TcIL3000_0_00060   endoplasmic reticulum oxidoreductin, putative
Trypanosoma cruzi TcCLB.508837.189   endoplasmic reticulum oxidoreductin, putative
Toxoplasma gondii TGME49_300380   endoplasmic reticulum oxidoreductin, putative
Theileria parva TP03_0411   hypothetical protein

Essentiality

TcCLB.508837.189 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.4890 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.4890 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.4890 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.4890 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y105E8B.8 Caenorhabditis elegans larval arrest wormbase
YML130C Saccharomyces cerevisiae inviable yeastgenome
TGME49_300380 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens ERO1-like (S. cerevisiae) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.508837.189 (Trypanosoma cruzi), endoplasmic reticulum oxidoreductin, putative
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