Detailed view for TcIL3000_10_14020

Basic information

TDR Targets ID: 560384
Trypanosoma congolense, cytoskeleton-associated protein CAP5.5, putative
Source Database / ID: 

pI: 4.1141 | Length (AA): 905 | MW (Da): 98589 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00648   Calpain family cysteine protease
PF09149   Domain of unknown function (DUF1935)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0004198   calcium-dependent cysteine-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 13 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
10 75 5ctd (B) 3 68 24.00 0 0.46 0.380728 -0.64
12 49 5cva (A) 4 40 46.00 0.27 0.12 0.572689 -0.87
15 48 1nay (A) 107 141 59.00 0.003 0.12 0.409269 -0.78
80 203 2fe0 (A) 5 131 31.00 0 1 0.530117 -0.11
88 197 1r75 (A) 13 131 30.00 0 0.99 0.471347 -0.5
213 541 1zcm (A) 33 353 22.00 0 1 0.674636 -0.39
223 698 1qxp (B) 33 552 22.00 0 1 0.551767 0.82
223 533 1qxp (A) 33 335 31.00 0 1 0.557346 0.58
223 549 3bow (A) 33 351 24.00 0 1 0.605126 -0.1
732 859 5cwo (A) 23 150 17.00 0 0.06 0.287236 -0.12
747 854 5cwp (A) 5 112 34.00 0.025 0.65 0.379037 0.27
755 802 4x8w (H) 1 307 63.00 0.00036 0.24 0.384739 3.14
757 852 5cwi (A) 130 233 35.00 0.29 0.91 0.394777 0.64

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_139833)

Species Accession Gene Product
Leishmania braziliensis LbrM.31.0600   cytoskeleton-associated protein CAP5.5, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania donovani LdBPK_310450.1   cytoskeleton-associated protein CAP5.5, putative
Leishmania infantum LinJ.31.0450   cytoskeleton-associated protein CAP5.5, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.31.0440   cytoskeleton-associated protein CAP5.5, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania mexicana LmxM.30.0440b  
Leishmania mexicana LmxM.30.0440  
Trypanosoma brucei gambiense Tbg972.4.4060   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei gambiense Tbg972.8.8670   calpain, putative,cysteine peptidase, putative
Trypanosoma brucei Tb927.4.3950   cytoskeleton-associated protein CAP5.5, putative
Trypanosoma brucei Tb927.8.8330   cysteine peptidase, putative
Trypanosoma congolense TcIL3000_10_14020   cytoskeleton-associated protein CAP5.5, putative
Trypanosoma cruzi TcCLB.511333.4   cytoskeleton-associated protein CAP5.5, putative
Trypanosoma cruzi TcCLB.509237.130   cytoskeleton-associated protein CAP5.5, putative
Trypanosoma cruzi TcCLB.508555.60   cytoskeleton-associated protein CAP5.5, putative

Essentiality

TcIL3000_10_14020 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.3950 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.3950 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.3950 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.3950 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.8330 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.8330 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.8330 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.8330 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Leishmania major cytoskeleton-associated protein CAP5.5, putative,cysteine peptidase, Clan CA, family C2, putative Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcIL3000_10_14020 (Trypanosoma congolense), cytoskeleton-associated protein CAP5.5, putative
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