Detailed view for Rv2471

Basic information

TDR Targets ID: 5644
Mycobacterium tuberculosis, Probable alpha-glucosidase AglA (maltase) (glucoinvertase) (glucosidosucrase) (maltase-glucoamylase) (lysosomal alpha-glucosidas
Source Database / ID:  Tuberculist 

pI: 4.7192 | Length (AA): 546 | MW (Da): 60444 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00128   Alpha amylase, catalytic domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0043169   cation binding  
GO:0003824   catalytic activity  
GO:0005975   carbohydrate metabolic process  

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 546 3m07 (A) 80 587 18.00 0 1 0.992 0.27
18 157 6bs6 (A) 45 181 47.00 0 1 0.80691 -0.21
31 545 3wy2 (A) 7 535 41.00 0 1 1.43372 -0.61
35 131 2ya0 (A) 133 262 33.00 0.26 1 0.331156 -0.74
35 134 3faw (A) 613 744 36.00 0.0082 1 0.37165 -0.59

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Dormant phase. hasan
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_126827)

Species Accession Gene Product
Brugia malayi Bm1_01300   Alpha amylase, catalytic domain containing protein
Brugia malayi Bm1_42675   Alpha amylase, catalytic domain containing protein
Candida albicans CaO19.11465   one of two alpha amylase genes similar to P.angusta Mal1p and to S. cerevisiae YGR287C
Candida albicans CaO19.3982   one of two alpha amylase genes similar to P.angusta Mal1p and to S. cerevisiae YGR287C
Candida albicans CaO19.7668   maltase gene whose transcription is induced by maltose and sucrose and repressed by glucose
Caenorhabditis elegans CELE_C38C6.2   Protein ATGP-2, isoform C
Caenorhabditis elegans CELE_F26D10.9   Protein ATGP-1, isoform B
Dictyostelium discoideum DDB_G0282715   hypothetical protein
Drosophila melanogaster Dmel_CG30359   Maltase A5
Drosophila melanogaster Dmel_CG8693   Maltase A4
Drosophila melanogaster Dmel_CG8696   Maltase A1
Drosophila melanogaster Dmel_CG14934   Maltase B1
Drosophila melanogaster Dmel_CG14935   Maltase B2
Drosophila melanogaster Dmel_CG8695   Maltase A3
Drosophila melanogaster Dmel_CG30360   Maltase A6
Drosophila melanogaster Dmel_CG8694   Maltase A2
Drosophila melanogaster Dmel_CG11669   Maltase A7
Drosophila melanogaster Dmel_CG8690   Maltase A8
Escherichia coli b4239   trehalose-6-P hydrolase
Echinococcus granulosus EgrG_000494800   alpha glucosidase
Entamoeba histolytica EHI_130690   oligo-1,6-glucosidase, putative
Echinococcus multilocularis EmuJ_000494800   alpha glucosidase
Homo sapiens 6519   solute carrier family 3 (amino acid transporter heavy chain), member 1
Loa Loa (eye worm) LOAG_01872   alpha amylase
Loa Loa (eye worm) LOAG_07109   alpha amylase
Mycobacterium leprae ML2045   Putative uncharacterized protein ML2045
Mus musculus ENSMUSG00000024131   solute carrier family 3, member 1
Mycobacterium tuberculosis Rv2471   Probable alpha-glucosidase AglA (maltase) (glucoinvertase) (glucosidosucrase) (maltase-glucoamylase) (lysosomal alpha-glucosidas
Mycobacterium tuberculosis Rv0126   Trehalose synthase TreS
Mycobacterium ulcerans MUL_4797   trehalose synthase TreS
Saccharomyces cerevisiae YGR292W   alpha-glucosidase MAL12
Saccharomyces cerevisiae YJL221C   oligo-1,6-glucosidase IMA4
Saccharomyces cerevisiae YIL172C   oligo-1,6-glucosidase IMA3
Saccharomyces cerevisiae YJL216C   oligo-1,6-glucosidase IMA5
Saccharomyces cerevisiae YOL157C   oligo-1,6-glucosidase IMA2
Saccharomyces cerevisiae YBR299W   alpha-glucosidase MAL32
Saccharomyces cerevisiae YGR287C   oligo-1,6-glucosidase IMA1
Schistosoma japonicum Sjp_0212450   ko:K01226 trehalose-6-phosphate hydrolase [EC3.2.1.93], putative
Schistosoma japonicum Sjp_0077040   ko:K01238 putative glycosyl hydrolase, family 13; putative alpha-glucosidase [EC:3.2.1.-], putative
Schistosoma japonicum Sjp_0004240   ko:K01182 oligo-1,6-glucosidase [EC3.2.1.10], putative
Schistosoma mansoni Smp_037540.2   alpha-amylase
Schistosoma mansoni Smp_052510   alpha-amylase
Schistosoma mansoni Smp_142810   alpha-amylase
Schistosoma mansoni Smp_156550   alpha-amylase
Schistosoma mansoni Smp_037540.1   alpha-amylase
Schmidtea mediterranea mk4.066224.00   4F2 cell-surface antigen heavy chain

Essentiality

Rv2471 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2514 this record Mycobacterium tuberculosis non-essential nmpdr
mtu127 Mycobacterium tuberculosis non-essential nmpdr
b4239 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Saccharomyces cerevisiae oligo-1,6-glucosidase IMA1 Compounds References
Saccharomyces cerevisiae alpha-glucosidase MAL12 Compounds References
Geobacillus stearothermophilus Alpha-glucosidase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0315 1 1
0.024 0.27 1
0.0144 0.4649 0.5
0.0072 1 0.5
0.0149 1 1
0.0312 1 1
0.0319 1 1
0.0224 0.5 0.5
0.008 0.4133 1
0.022 1 1
0.0077 0.5 0.5
0.0074 0.5963 0.5
0.0145 1 0.5
0.0148 0.6198 0.5958
0.0158 0.3656 0.5
0.0235 0.8065 1
0.0224 0.5 0.5
0.0344 0.8694 0.5
0.0146 0.4876 0.5
0.0325 0.5 0.5
0.0314 1 1
0.0261 1 1
0.0261 0.2587 1
0.0261 1 1
0.0085 0.5 0.5
0.0151 0.8892 0.5
0.0261 0.2587 1
0.0157 0.4186 1
0.0229 1 1
0.0344 1 1
0.0261 0.2503 1
0.0344 0.5 0.5
0.0073 1 1
0.0103 0.3114 1
0.0261 0.2587 1
0.0261 1 1
0.0262 0.2775 1
0.0126 0.3182 1
0.0074 0.5963 0.5
0.0087 1 1
0.00656587 0.5 0.5
0.0089 0.3085 1
0.0344 0.8694 0.5

Assayability

Assay information

  • Assay for Maltase (3.2.1.20 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

6 literature references were collected for this gene.

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Gene identifier Rv2471 (Mycobacterium tuberculosis), Probable alpha-glucosidase AglA (maltase) (glucoinvertase) (glucosidosucrase) (maltase-glucoamylase) (lysosomal alpha-glucosidas
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