Detailed view for Rv1538c

Basic information

TDR Targets ID: 5663
Mycobacterium tuberculosis, Probable L-aparaginase AnsA
Source Database / ID:  Tuberculist 

pI: 4.8324 | Length (AA): 326 | MW (Da): 32947 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00710   Asparaginase, N-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0006520   amino acid metabolic process  

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 325 1zq1 (A) 85 419 25.00 0 1 1.2021 0.14
12 326 5mq5 (A) 1 324 29.00 0 1 1.28976 -0.59
14 321 5ot0 (A) 2 313 32.00 0 1 1.34449 -0.51
14 321 5k4g (A) 5 323 30.00 0 1 1.28249 -0.44
201 270 5vmt (A) 86 150 42.00 0.52 0.87 0.498424 0.72

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_127035)

Species Accession Gene Product
Brugia malayi Bm1_50405   L-asparaginase
Candida albicans CaO19.7593   likely asparaginase similar to S. cerevisiae ASP1 (YDR321W)
Caenorhabditis elegans CELE_C27A7.5   Protein C27A7.5, isoform D
Dictyostelium discoideum DDB_G0271924   L-asparaginase I
Drosophila melanogaster Dmel_CG8526   CG8526 gene product from transcript CG8526-RA
Drosophila melanogaster Dmel_CG6428   CG6428 gene product from transcript CG6428-RA
Escherichia coli b2957   periplasmic L-asparaginase 2
Escherichia coli b1767   cytoplasmic L-asparaginase 1
Echinococcus granulosus EgrG_000796600   Asparaginase glutaminase
Echinococcus granulosus EgrG_001165500   Ankyrin
Entamoeba histolytica EHI_178920   L-asparaginase, putative
Entamoeba histolytica EHI_050740   L-asparaginase, putative
Echinococcus multilocularis EmuJ_000796600   Asparaginase glutaminase
Echinococcus multilocularis EmuJ_001165500   Ankyrin
Giardia lamblia GL50803_14547   L-asparaginase
Homo sapiens ENSG00000166183   asparaginase
Leishmania braziliensis LbrM.35.4670   asparaginase-like protein
Leishmania braziliensis LbrM.15.0410   cytoplasmic l-asparaginase i-like protein
Leishmania donovani LdBPK_150440.1   cytoplasmic l-asparaginase i-like protein
Leishmania donovani LdBPK_364650.1   asparaginase-like protein
Leishmania infantum LinJ.36.4650   asparaginase-like protein
Leishmania infantum LinJ.15.0440   cytoplasmic l-asparaginase i-like protein
Leishmania major LmjF.36.4430   asparaginase-like protein
Leishmania major LmjF.15.0390   cytoplasmic l-asparaginase i-like protein
Leishmania mexicana LmxM.15.0390   cytoplasmic l-asparaginase i-like protein
Leishmania mexicana LmxM.36.4430   asparaginase-like protein
Loa Loa (eye worm) LOAG_00838   hypothetical protein
Mycobacterium leprae ML1198c   Probable L-aparaginase AnsA
Mus musculus ENSMUSG00000037686   asparaginase homolog (S. cerevisiae)
Mycobacterium tuberculosis Rv1538c   Probable L-aparaginase AnsA
Mycobacterium ulcerans MUL_1536   L-aparaginase AnsA
Neospora caninum NCLIV_041200   asparaginase, putative
Saccharomyces cerevisiae YLR155C   asparaginase ASP3-1
Saccharomyces cerevisiae YDR321W   asparaginase ASP1
Saccharomyces cerevisiae YLR160C   asparaginase ASP3-4
Saccharomyces cerevisiae YLR157C   asparaginase ASP3-2
Saccharomyces cerevisiae YLR158C   asparaginase ASP3-3
Schistosoma japonicum Sjp_0109050   IPR002110,Ankyrin,domain-containing
Schistosoma japonicum Sjp_0106020   ko:K01048 lysophospholipase [EC3.1.1.5], putative
Schistosoma japonicum Sjp_0003290   ko:K01048 lysophospholipase [EC3.1.1.5], putative
Schistosoma mansoni Smp_153810   l-asparaginase I
Schmidtea mediterranea mk4.027277.00   Putative l-asparaginase I
Schmidtea mediterranea mk4.034237.03  
Schmidtea mediterranea mk4.059437.00  
Schmidtea mediterranea mk4.002597.00   Putative l-asparaginase I
Trypanosoma cruzi TcCLB.504147.110   asparaginase, putative

Essentiality

Rv1538c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1566 this record Mycobacterium tuberculosis non-essential nmpdr
b1767 Escherichia coli non-essential goodall
b2957 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Asparaginase (3.5.1.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

33 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv1538c (Mycobacterium tuberculosis), Probable L-aparaginase AnsA
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