Detailed view for Rv1266c

Basic information

TDR Targets ID: 6800
Mycobacterium tuberculosis, Probable transmembrane serine/threonine-protein kinase H PknH (protein kinase H) (STPK H)
Source Database / ID:  Tuberculist 

pI: 6.7203 | Length (AA): 626 | MW (Da): 66754 | Paralog Number: 3

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 1

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF14032   PknH-like extracellular domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
14 277 2h34 (A) 14 276 70.00 0 1 1.17483 -0.49
16 371 4yzb (A) 28 381 18.00 0 1 0.71459 0.07
291 401 5i04 (A) 604 717 5.00 0 0 0.292216 -1.36
433 626 4esq (A) 433 626 98.00 0 1 1.553 -1.79

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 1MRU:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1O6Y:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2FUM:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2H34:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3F61:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3F69:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3ORI:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3ORK:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3ORL:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3ORM:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3ORO:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3ORP:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3ORT:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4ESQ:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4OW8:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4X3F:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 5U94:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 6B2P:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 6B2Q:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_142551)

Species Accession Gene Product
Giardia lamblia GL50803_9421   Kinase, NEK
Giardia lamblia GL50803_9665   Kinase, NEK
Mycobacterium tuberculosis Rv2088   Transmembrane serine/threonine-protein kinase J PknJ (protein kinase J) (STPK J)
Mycobacterium tuberculosis Rv1743   Probable transmembrane serine/threonine-protein kinase E PknE (protein kinase E) (STPK E)
Mycobacterium tuberculosis Rv1266c   Probable transmembrane serine/threonine-protein kinase H PknH (protein kinase H) (STPK H)
Mycobacterium tuberculosis Rv2914c   Probable transmembrane serine/threonine-protein kinase I PknI (protein kinase I) (STPK I) (phosphorylase B kinase kinase) (hydro
Mycobacterium ulcerans MUL_3180   Ser/Thr protein kinase
Mycobacterium ulcerans MUL_2042   Ser/Thr protein kinase

Essentiality

Rv1266c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1773 Mycobacterium tuberculosis non-essential nmpdr
mtu1287 this record Mycobacterium tuberculosis non-essential nmpdr
mtu2961 Mycobacterium tuberculosis non-essential nmpdr
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) normal (PATO:0000461) inferred from animal model system (ECO:0000179) Mycobacterium tuberculosis No drug identifiers listed for this gene.
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 16077122
growth (GO:0040007) normal (PATO:0000461) inferred from in vitro culture assay (ECO:0000182) Mycobacterium tuberculosis No drug identifiers listed for this gene.
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 16077122
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Mycobacterium tuberculosis 34576   55244   132331   161165   568050   574748   574749  
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 15043876

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Sus scrofa Casein kinase I isoform alpha 125 aa 29.9% 127 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0066 0.3101 0.5
0.0008 0.5 0.5
0.0011 1 0.5
0.0033 0.5 0.5
0.0063 1 1
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0092 1 0.5
0.0039 0.5 0.5
0.0063 0.7244 1
0.0007 0.5 0.5
0.0004 0.5 0.5
0.0003 0.5 0.5
0.0059 1 0.5
0.0036 0.5 0.5
0.0007 0.5 0.5
0.0039 0.9485 1
0.0062 0.6935 0.5
0.0032 0.5 0.5
0.0033 1 1
0.0032 0.5 0.5
0.0061 0.6883 0.6563
0.0042 0.5 0.5
0.0064 0.3377 0.5
0.0018 0.5 0.5
0.0081 1 0.5
0.0059 1 0.5
0.0022 0.5 0.5
0.0093 0.8828 0.5
0.0012 0.5 0.5
0.0027 1 0.5
0.0081 0.5 0.5
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0091 1 0.5
0.0012 0.5 0.5
0.0059 1 0.5
0.0088 0.4477 1
0.0069 0.3067 1
0.0098 0.3242 0.3676
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0039 0.5 0.5
0.0029 0.5 0.5
0.0037 1 0.5
0.0056 1 0.5
0.0026 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

81 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv1266c (Mycobacterium tuberculosis), Probable transmembrane serine/threonine-protein kinase H PknH (protein kinase H) (STPK H)
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