Detailed view for Rv0208c

Basic information

TDR Targets ID: 7227
Mycobacterium tuberculosis, Hypothetical methlytransferase (methylase)

Source Database / ID: Tuberculist / Rv0208c 

pI: 9.8494 | Length (AA): 263 | MW (Da): 28547 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Pfam domains

PF02390   Putative methyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0008176   tRNA (guanine-N7-)-methyltransferase activity  
GO:0006400   tRNA modification  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
14 226 3c3y (A) 13 227 22.00 0 1 0.962286 0.2
44 252 3dxy (A) 36 237 37.00 0 1 1.30328 -0.78

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Shows this level of upregulation in
This gene ranks in the upper 60-80% group of genes. Dormant phase.
Upregulation Percent Ranking Shows this level of upregulation in
This gene ranks in the lower 20-40% group of genes. Dormant phase.
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_127179)

Species Accession Gene Product
Arabidopsis thaliana AT5G17660   tRNA (guanine-N-7) methyltransferase
Arabidopsis thaliana AT5G24840   tRNA (guanine-N(7)-)-methyltransferase
Brugia malayi Bm1_01445   tRNA (guanine-N(7)-)-methyltransferase
Candida albicans CaO19.3798   potential methyltransferase similar to S. cerevisiae TRM8 (YDL201W) tRNA methyltransferase
Candida albicans CaO19.11279   potential methyltransferase similar to S. cerevisiae TRM8 (YDL201W) tRNA methyltransferase
Caenorhabditis elegans CELE_W02B12.10   Protein W02B12.10
Cryptosporidium hominis Chro.70029   methyltransferase
Cryptosporidium parvum cgd7_160   putative methyltransferase-related
Dictyostelium discoideum DDB_G0276491   tRNA -methyltransferase
Drosophila melanogaster Dmel_CG4045   CG4045 gene product from transcript CG4045-RA
Escherichia coli b2960   tRNA m(7)G46 methyltransferase, SAM-dependent
Echinococcus granulosus EgrG_000057400   tRNA guanine N7 methyltransferase
Entamoeba histolytica EHI_178940   methyltransferase, putative
Echinococcus multilocularis EmuJ_000057400   tRNA (guanine N(7)) methyltransferase
Giardia lamblia GL50803_10335   tRNA methyltransferase subunit, putative
Homo sapiens ENSG00000037897   methyltransferase like 1
Leishmania braziliensis LbrM.34.5180   tRNA (guanine-N(7)-)-methyltransferase, putative,methyltransferase, putative
Leishmania donovani LdBPK_355200.1   tRNA (guanine-N(7)-)-methyltransferase, putative
Leishmania infantum LinJ.35.5200   tRNA (guanine-N(7)-)-methyltransferase, putative,methyltransferase, putative
Leishmania major LmjF.35.5230   tRNA (guanine-N(7)-)-methyltransferase, putative,methyltransferase, putative
Leishmania mexicana LmxM.34.5230   tRNA (guanine-N(7)-)-methyltransferase, putative,methyltransferase, putative
Loa Loa (eye worm) LOAG_00685   tRNA guanine-N7 methyltransferase
Mycobacterium leprae ML2622c   Probable tRNA (guanine-N(7)-)-methyltransferase (tRNA(m7G46)-methyltransferase )
Mus musculus ENSMUSG00000006732   methyltransferase like 1
Mycobacterium tuberculosis Rv0208c this record   Hypothetical methlytransferase (methylase)
Mycobacterium ulcerans MUL_1098   tRNA (guanine-N(7)-)-methyltransferase
Neospora caninum NCLIV_000920   tRNA (guanine-N(7)-)-methyltransferase, putative
Oryza sativa 4326418   Os01g0535900
Oryza sativa 4340601   Os06g0236900
Plasmodium berghei PBANKA_0921100   tRNA (guanine-N(7)-)-methyltransferase, putative
Plasmodium falciparum PF3D7_1127300   tRNA (guanine-N(7)-)-methyltransferase, putative
Plasmodium knowlesi PKNH_0925300   tRNA (guanine-N(7)-)-methyltransferase, putative
Plasmodium vivax PVX_091980   tRNA (guanine-N(7)-)-methyltransferase, putative
Plasmodium yoelii PY05818   Putative Methyltransferase-related
Saccharomyces cerevisiae YDL201W   Trm8p
Schistosoma japonicum Sjp_0303790   ko:K03439 tRNA (guanine-N7-)-methyltransferase [EC2.1.1.33], putative
Schistosoma mansoni Smp_130610   tRNA (guanine-N(7)-)-methyltransferase
Schmidtea mediterranea mk4.002174.10   tRNA
Trypanosoma brucei gambiense Tbg972.9.5070   tRNA (guanine-N(7)-)-methyltransferase, putative,methyltransferase, putative
Trypanosoma brucei Tb927.9.9120   tRNA (guanine-N(7)-)-methyltransferase, putative
Trypanosoma congolense TcIL3000_9_3200   tRNA (guanine-N(7)-)-methyltransferase, putative
Trypanosoma cruzi TcCLB.510429.20   tRNA (guanine-N(7)-)-methyltransferase, putative
Trypanosoma cruzi TcCLB.506883.80   tRNA (guanine-N(7)-)-methyltransferase, putative
Treponema pallidum TP0464   hypothetical protein
Theileria parva TP01_0377   methyltransferase, putative
Trichomonas vaginalis TVAG_069970   3-deoxy-D-manno-octulosonic-acid transferase/tRNA (guanine-N(7)-)-methyltransferase, putative
Trichomonas vaginalis TVAG_166600   3-deoxy-D-manno-octulosonic-acid transferase/tRNA (guanine-N(7)-)-methyltransferase, putative

Essentiality

Rv0208c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu210 this record Mycobacterium tuberculosis essential nmpdr
Tb09.211.0790 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.0790 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.0790 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.0790 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2960 Escherichia coli non-essential goodall
CELE_W02B12.10 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_0921100 Plasmodium berghei Dispensable plasmo
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
 
In any case, if you have information about papers containing relevant validation data for this target, please log in using your TDR Targets username and password and send them to us using the corresponding form in this page (only visible to registered users) or contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Sus scrofa Catechol O-methyltransferase 186 aa 21.4% 154 aa Compounds References

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

63 literature references were collected for this gene.

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Gene identifier Rv0208c (Mycobacterium tuberculosis), Hypothetical methlytransferase (methylase)
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